低温处理虾夷扇贝氧化与品质特性研究
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- 英文论文题名：Study on oxidation and quality characteristics of patinopecten yessoensis at low-temperature heating
- 论文作者：傅卉 ; 常思佳 ; 包汭琪 ; 程洋 ; 董秀芳 ; 启航
- 英文论文作者：Fu Hui ; Chang Sijia ; Bao Ruiqi ; Cheng Yang ; Dong Xiufang ; Qi Hang ; School of Food Science and Technology ; Dalian Polytechnic University ; National Engineering Research Center of Seafood
- 年：2016
- 作者机构：大连工业大学食品学院;国家海洋食品工程技术研究中心;
- 论文关键词：虾夷扇贝 ; 闭壳肌 ; 低温热处理 ; 氧化 ; 蛋白质
- 英文论文关键词：patinopecten yessoensis ; adductor muscle ; low temperature heat treatment ; oxidation ; protein
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS254.4
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：116k
- 原文格式：O
- 会议级别：全国
摘要
以新鲜的虾夷扇贝闭壳肌为原料,55℃加热不同时间(1/6,1/2,1,2,4,6,12,18,21,24,27,30,32h)研究虾夷扇贝闭壳肌抗氧化酶活力、钙镁-ATP酶活力、组织蛋白酶L活力、Caspase-3活力、巯基、丙二醛和羰基含量的变化,采用SDS-PAGE与质构分析技术研究蛋白质与质构的变化。结果表明:随着热处理时间的延长,超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶的比酶活、钙镬-ATP酶活性及巯基的相对含量分别减弱约44.62%、56.34%、68.17%、56.72%、70.39%;组织蛋白酶L、Caspase-3酶活力、丙二醛和羰基的相对含量增加约22.72%、11.48%、46.27%和12.13%。SDS-PAGE与质构评定结果表明随着加热时间的延长,虾夷扇贝闭壳肌的高分子量蛋白发生明显降解,质构特性发生明显变化。综上结果表明虾夷扇贝闭壳肌在低温处理过程中发生氧化应激反应,蛋白质在一定程度上发生变化,质构特性发生明显变化。本研究对于保持虾夷扇贝加工产品的品质,提高产品价值具有一定的现实意义。
Adductor muscle of fresh patinopecten yessoensis as raw materials,were based on heating different time at 55 ℃(1/6,1/2,1,2,4,6,12,18,21,24,27,30,32h),to study the activity of antioxidant enzyme,Ca~(2+) Mg~(2+)- ATP enzyme,cathepsin L,caspase-3,the content of thiol,MDA and carbonyl,the change of protein and texture using SDS-PAGE and texture analysis technique.The results showed that with the extension of heating time,the activity of superoxide dismutase,catalase and glutathione peroxidase,Ca~(2+)Mg~(2+)- ATPase and the content of thiol were decreased by about 44.62,56.34%,68.17%,56.72%,70.39%,respectively;The activity of cathepsin L and caspase-3 enzyme,the concent of malondialdehyde and carbonyl were increased about 22.72%,11.48%,46.27%,12.13%,respectively.The results of SDS-PAGE and texture evaluation showed that with the extension of heating time,high molecular weight protein in adductor muscle of patinopecten yessoensis occured obvious degradation,textural properties changed obviously.In summary,the results showed that adductor muscle of patinopecten yessoensis in treatment of low temperature occurred the reaction of oxidation stress;Protein changed in a certain extent;Textural properties changed obviously.In this study,the quality of patinopecten yessoensis processing products for maintaining and the value of the product for improving had practical significance.
Adductor muscle of fresh patinopecten yessoensis as raw materials,were based on heating different time at 55 ℃(1/6,1/2,1,2,4,6,12,18,21,24,27,30,32h),to study the activity of antioxidant enzyme,Ca~(2+) Mg~(2+)- ATP enzyme,cathepsin L,caspase-3,the content of thiol,MDA and carbonyl,the change of protein and texture using SDS-PAGE and texture analysis technique.The results showed that with the extension of heating time,the activity of superoxide dismutase,catalase and glutathione peroxidase,Ca~(2+)Mg~(2+)- ATPase and the content of thiol were decreased by about 44.62,56.34%,68.17%,56.72%,70.39%,respectively;The activity of cathepsin L and caspase-3 enzyme,the concent of malondialdehyde and carbonyl were increased about 22.72%,11.48%,46.27%,12.13%,respectively.The results of SDS-PAGE and texture evaluation showed that with the extension of heating time,high molecular weight protein in adductor muscle of patinopecten yessoensis occured obvious degradation,textural properties changed obviously.In summary,the results showed that adductor muscle of patinopecten yessoensis in treatment of low temperature occurred the reaction of oxidation stress;Protein changed in a certain extent;Textural properties changed obviously.In this study,the quality of patinopecten yessoensis processing products for maintaining and the value of the product for improving had practical significance.
引文