Involvement of autophagy in regulating chloroplast development in Arabidopsis
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- 英文论文题名：Involvement of autophagy in regulating chloroplast development in Arabidopsis
- 论文作者：Qingjun Xie
- 英文论文作者：Qingjun Xie ; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources ; Guangdong Provincial Key Lab of Plant Molecular Breeding ; South China Agricultural University
- 年：2016
- 作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangdong Provincial Key Lab of Plant Molecular Breeding,South China Agricultural University;
- 英文论文关键词：autophagy ; ATG8 ; chloroplast development ; ATI1 ; RCB
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q945
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Autophagy plays multiple roles in the plastid degradation during senescence and/or stresses,particularly in the chloroplast. However,relatively little is known about the role of autophagy in the developmental process of chloroplasts. Here,we characterized the relationship between autophagy and a chloroplast protein(CP1),which was identified to interact with Atg8 from a yeast two hybrid screen. Our results further revealed that CP1 also interacts with Atg8 in planta,and Atg5 and Atg7 are required for the autophagy-mediated degradation of CP1. Nevertheless,our results also indicated that the interaction between Atg8 and CP1 was controlled by a specific Atg8-interacting motif(AIM). So far,there are two mechanisms,ATI1-PS and Rubisco-containing body(RCB),controlling the plastid proteins trafficking from plastids to the vacuole in the autophagy-dependent manner. In this study,we found that autophagy-mediated transport of CP1 from plastid to the vacuole is independent on both ATI1-PS and RCB pathways,suggesting a novel unknown mechanism underlying this process. In addition,two other proteins CP2 and CP3 that has been found to regulate chloroplast development were also evident to be turned over by autophagy. Therefore,we propose that autophagy may be involved in the chloroplast development through the regulation of the homeostasis of a subset of chloroplast proteins in plant.
Autophagy plays multiple roles in the plastid degradation during senescence and/or stresses,particularly in the chloroplast. However,relatively little is known about the role of autophagy in the developmental process of chloroplasts. Here,we characterized the relationship between autophagy and a chloroplast protein(CP1),which was identified to interact with Atg8 from a yeast two hybrid screen. Our results further revealed that CP1 also interacts with Atg8 in planta,and Atg5 and Atg7 are required for the autophagy-mediated degradation of CP1. Nevertheless,our results also indicated that the interaction between Atg8 and CP1 was controlled by a specific Atg8-interacting motif(AIM). So far,there are two mechanisms,ATI1-PS and Rubisco-containing body(RCB),controlling the plastid proteins trafficking from plastids to the vacuole in the autophagy-dependent manner. In this study,we found that autophagy-mediated transport of CP1 from plastid to the vacuole is independent on both ATI1-PS and RCB pathways,suggesting a novel unknown mechanism underlying this process. In addition,two other proteins CP2 and CP3 that has been found to regulate chloroplast development were also evident to be turned over by autophagy. Therefore,we propose that autophagy may be involved in the chloroplast development through the regulation of the homeostasis of a subset of chloroplast proteins in plant.
Autophagy plays multiple roles in the plastid degradation during senescence and/or stresses,particularly in the chloroplast. However,relatively little is known about the role of autophagy in the developmental process of chloroplasts. Here,we characterized the relationship between autophagy and a chloroplast protein(CP1),which was identified to interact with Atg8 from a yeast two hybrid screen. Our results further revealed that CP1 also interacts with Atg8 in planta,and Atg5 and Atg7 are required for the autophagy-mediated degradation of CP1. Nevertheless,our results also indicated that the interaction between Atg8 and CP1 was controlled by a specific Atg8-interacting motif(AIM). So far,there are two mechanisms,ATI1-PS and Rubisco-containing body(RCB),controlling the plastid proteins trafficking from plastids to the vacuole in the autophagy-dependent manner. In this study,we found that autophagy-mediated transport of CP1 from plastid to the vacuole is independent on both ATI1-PS and RCB pathways,suggesting a novel unknown mechanism underlying this process. In addition,two other proteins CP2 and CP3 that has been found to regulate chloroplast development were also evident to be turned over by autophagy. Therefore,we propose that autophagy may be involved in the chloroplast development through the regulation of the homeostasis of a subset of chloroplast proteins in plant.
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