Wild-type Rabies Virus Induces Autophagy in both SK and NA cells
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- 英文论文题名：Wild-type Rabies Virus Induces Autophagy in both SK and NA cells
- 论文作者：Jiaojiao Peng ; Shenghe Zhu ; Lili Hu ; Pingping Ye ; Yifei Wang ; Qin Tian ; Mingzhu Mei ; Xiaofeng Guo
- 英文论文作者：Jiaojiao Peng ; Shenghe Zhu ; Lili Hu ; Pingping Ye ; Yifei Wang ; Qin Tian ; Mingzhu Mei ; Xiaofeng Guo ; College of Veterinary Medicine ; South China Agricultural University
- 年：2016
- 作者机构：College of Veterinary Medicine,South China Agricultural University;
- 英文论文关键词：autophagy ; rabies virus ; matrix protein ; autophagosome ; autophagic flux ; apoptosis
- 会议召开时间：2016-09-18
- 会议录名称：第六届中国兽药大会论文集
- 英文会议录名称：Proceedings of 2016 the 6th China Congress of Veterinary Medicine
- 语种：英文
- 分类号：S852.65
- 学会代码：ZGXJ
- 会议名称：第六届中国兽药大会
- 会议地点：中国四川成都
- 主办单位：中国兽医药品监察所、中国兽药协会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：58k
- 原文格式：O
- 会议级别：全国
摘要
Different Rabies virus(RABV) strains have their own biological characteristics,but little is known about their respective impact on autophagy.In this study,attenuated RABV HEPFlury and wild-type RABV GD-SH-01 strains have been selected based on their very different biological characteristics.We found that GD-SH-01 infection significantly increases the number of autophagy-like vesicles,the accumulation of EGFP-LC3 fluorescence puncta and the conversion of LC3-I to LC3-II,while HEP-Flury is not able to induce this phenomenon.Next,we confirmed that GD-SH-01 infection triggered a complete autophagic response in human neuroblastoma cell line(SK),but inhibited autophagosome fusion with lysosomes in mouse neuroblastoma cell line(NA).Moreover,GD-SH-01 induces apoptosis and mitochondrial dysfunction while triggering autophagy,and apoptosis can be decreased by enhancing autophagy.And AMPK.signaling pathway has been activated by GD-SH-01 in SK.In addition,five recombinant RABV were rescued based on the back-bone of HEP-Flury virus,whose genes were replaced respectively by the gene encoding single viral structure protein of the nucleoprotein(N),the phosphoprotein(P),the matrix protein(M),the glycoprotein(G),and the RNA-dependent RNA polymerase(L).We confirmed that rHEP-shM-infected cells triggered obviously LC3-Ⅰ/LC3-Ⅱ conversion,and minor autophagy showed in rHEP-shN,rHEP-shP,and rHEP-shG infected cells.We also verified that autophagy can not be induced by single structure protein of GD-SH-01.Therefore,our data provide strong evidence that autophagy is induced by GD-SH-01 and can decrease apoptosis in vitro.Furthermore,M of GD-SH-01 is not the only but a main protein for promoting RABV inducing autophagy.
Different Rabies virus(RABV) strains have their own biological characteristics,but little is known about their respective impact on autophagy.In this study,attenuated RABV HEPFlury and wild-type RABV GD-SH-01 strains have been selected based on their very different biological characteristics.We found that GD-SH-01 infection significantly increases the number of autophagy-like vesicles,the accumulation of EGFP-LC3 fluorescence puncta and the conversion of LC3-I to LC3-II,while HEP-Flury is not able to induce this phenomenon.Next,we confirmed that GD-SH-01 infection triggered a complete autophagic response in human neuroblastoma cell line(SK),but inhibited autophagosome fusion with lysosomes in mouse neuroblastoma cell line(NA).Moreover,GD-SH-01 induces apoptosis and mitochondrial dysfunction while triggering autophagy,and apoptosis can be decreased by enhancing autophagy.And AMPK.signaling pathway has been activated by GD-SH-01 in SK.In addition,five recombinant RABV were rescued based on the back-bone of HEP-Flury virus,whose genes were replaced respectively by the gene encoding single viral structure protein of the nucleoprotein(N),the phosphoprotein(P),the matrix protein(M),the glycoprotein(G),and the RNA-dependent RNA polymerase(L).We confirmed that rHEP-shM-infected cells triggered obviously LC3-Ⅰ/LC3-Ⅱ conversion,and minor autophagy showed in rHEP-shN,rHEP-shP,and rHEP-shG infected cells.We also verified that autophagy can not be induced by single structure protein of GD-SH-01.Therefore,our data provide strong evidence that autophagy is induced by GD-SH-01 and can decrease apoptosis in vitro.Furthermore,M of GD-SH-01 is not the only but a main protein for promoting RABV inducing autophagy.
Different Rabies virus(RABV) strains have their own biological characteristics,but little is known about their respective impact on autophagy.In this study,attenuated RABV HEPFlury and wild-type RABV GD-SH-01 strains have been selected based on their very different biological characteristics.We found that GD-SH-01 infection significantly increases the number of autophagy-like vesicles,the accumulation of EGFP-LC3 fluorescence puncta and the conversion of LC3-I to LC3-II,while HEP-Flury is not able to induce this phenomenon.Next,we confirmed that GD-SH-01 infection triggered a complete autophagic response in human neuroblastoma cell line(SK),but inhibited autophagosome fusion with lysosomes in mouse neuroblastoma cell line(NA).Moreover,GD-SH-01 induces apoptosis and mitochondrial dysfunction while triggering autophagy,and apoptosis can be decreased by enhancing autophagy.And AMPK.signaling pathway has been activated by GD-SH-01 in SK.In addition,five recombinant RABV were rescued based on the back-bone of HEP-Flury virus,whose genes were replaced respectively by the gene encoding single viral structure protein of the nucleoprotein(N),the phosphoprotein(P),the matrix protein(M),the glycoprotein(G),and the RNA-dependent RNA polymerase(L).We confirmed that rHEP-shM-infected cells triggered obviously LC3-Ⅰ/LC3-Ⅱ conversion,and minor autophagy showed in rHEP-shN,rHEP-shP,and rHEP-shG infected cells.We also verified that autophagy can not be induced by single structure protein of GD-SH-01.Therefore,our data provide strong evidence that autophagy is induced by GD-SH-01 and can decrease apoptosis in vitro.Furthermore,M of GD-SH-01 is not the only but a main protein for promoting RABV inducing autophagy.
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