Newcastle disease virus NP and P proteins induce autophagy via the endoplasmic reticulum stress-related unfolded protein response
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- 英文论文题名：Newcastle disease virus NP and P proteins induce autophagy via the endoplasmic reticulum stress-related unfolded protein response
- 论文作者：Jing-Hua Cheng ; Ying-Jie Sun ; Fan-Qing Zhang ; Xiao-Rong Zhang ; Xv-Sheng Qiu ; Li-Ping Yu ; Yantao Wu ; Chan Ding
- 英文论文作者：Jing-Hua Cheng ; Ying-Jie Sun ; Fan-Qing Zhang ; Xiao-Rong Zhang ; Xv-Sheng Qiu ; Li-Ping Yu ; Yantao Wu ; Chan DingShanghai Veterinary Research Institute ; CAAS ; Yangzhou University
- 年：2016
- 作者机构：Yangzhou University;Shanghai Veterinary Research Institute,CAAS;
- 英文论文关键词：Autophagy ; endoplasmic reticulum(ER) stress ; Newcastle disease virus ; unfolded protein response(UPR)
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：英文
- 分类号：S852.65
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：2
- 文件大小：450k
- 原文格式：D
- 会议级别：全国
摘要
Newcastle disease virus(NDV) can replicate and trigger autophagy in human tumor cells.Our previous study confirmed the critical role of autophagy in NDV infection.Here we studied the role of NDV structural proteins in the induction of autophagy through endoplasmic reticulum(ER) stress-related unfolded protein response(UPR) pathways.Ectopic expression of the NDV nucleocapsid protein(NP) or phosphoprotein(P) was sufficient to induce autophagy.NP or P expression also altered ER homeostasis.The PERK and ATF6 pathways,but not the XBP1 pathway,all of which are components of the UPR,were activated in both NDV-infected and NP or P-transfected cells.Knockdown of PERK or ATF6 inhibited NDV-induced autophagy and reduced the extent of NDV replication.Collectively,these data suggest not only roles for the NDV NP and P proteins in autophagy,but also offer new insights into the mechanisms of NDV-induced autophagy through activation of the ER stress-related UPR pathway.
Newcastle disease virus(NDV) can replicate and trigger autophagy in human tumor cells.Our previous study confirmed the critical role of autophagy in NDV infection.Here we studied the role of NDV structural proteins in the induction of autophagy through endoplasmic reticulum(ER) stress-related unfolded protein response(UPR) pathways.Ectopic expression of the NDV nucleocapsid protein(NP) or phosphoprotein(P) was sufficient to induce autophagy.NP or P expression also altered ER homeostasis.The PERK and ATF6 pathways,but not the XBP1 pathway,all of which are components of the UPR,were activated in both NDV-infected and NP or P-transfected cells.Knockdown of PERK or ATF6 inhibited NDV-induced autophagy and reduced the extent of NDV replication.Collectively,these data suggest not only roles for the NDV NP and P proteins in autophagy,but also offer new insights into the mechanisms of NDV-induced autophagy through activation of the ER stress-related UPR pathway.
Newcastle disease virus(NDV) can replicate and trigger autophagy in human tumor cells.Our previous study confirmed the critical role of autophagy in NDV infection.Here we studied the role of NDV structural proteins in the induction of autophagy through endoplasmic reticulum(ER) stress-related unfolded protein response(UPR) pathways.Ectopic expression of the NDV nucleocapsid protein(NP) or phosphoprotein(P) was sufficient to induce autophagy.NP or P expression also altered ER homeostasis.The PERK and ATF6 pathways,but not the XBP1 pathway,all of which are components of the UPR,were activated in both NDV-infected and NP or P-transfected cells.Knockdown of PERK or ATF6 inhibited NDV-induced autophagy and reduced the extent of NDV replication.Collectively,these data suggest not only roles for the NDV NP and P proteins in autophagy,but also offer new insights into the mechanisms of NDV-induced autophagy through activation of the ER stress-related UPR pathway.
引文