青海麝香对人胃癌细胞株裸鼠移植瘤Bcl-2、Bax表达的影响
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- 论文作者：王宏斌 ; 曹昌霞
- 年：2016
- 作者机构：青海大学研究生院;青海大学附属医院;
- 论文关键词：麝香 ; BGC-823 ; 细胞凋亡 ; Bcl-2 ; Bax
- 英文论文关键词：musk ; BGC-823 ; inhibition rate ; Bcl-2 ; Bax
- 会议召开时间：2016-11-25
- 会议录名称：湖南中医药大学学报2016/专集：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会论文集
- 语种：中文
- 分类号：R285.5
- 学会代码：HNZY
- 会议名称：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会
- 会议地点：中国广东珠海
- 主办单位：国际数字医学会、Digital Chinese Medicine
- 学会名称：湖南中医药大学期刊杂志社
- 页数：2
- 文件大小：2009k
- 原文格式：D
- 会议级别：全国
摘要
目的探讨青海麝香对人胃癌裸鼠移植瘤Bcl-2及Bax表达的影响及相关机制。方法通过皮下注射细胞悬液建立人胃癌移植瘤模型,将35只裸鼠随机分为模型对照组、氟尿嘧啶组、麝香高剂量组、麝香中剂量组、麝香低剂量组,按照给药方案用药。每用药5d测量一次裸鼠瘤体大小并计算每组平均瘤体积。用药21d后处死动物,剥取肿瘤,计算瘤体质量,免疫组化法检测瘤体组织Bcl-2、Bax的表达。结果与模型对照组相比,给药各组对人胃癌移植瘤生长均出现不同程度的抑制,(P<0.05)。与模型对照组相比,麝香高、中、低剂量组及氟尿嘧啶组的移植瘤质量明显低于模型组,(P<0.05)。麝香高剂量组、麝香中剂量组Bcl-2平均灰度值与模型对照组比较,均显著偏低,(P<0.05);各用药组平均面密度较模型对照组比较,无明显差异,(P>0.05)。麝香高剂量组、麝香中剂量组平均灰度值与模型对照组比较,均显著升高,(P<0.05);麝香各用药组平均面密度较模型对照组均显著增加,(P<0.05)。结论麝香其具有抑制部分肿瘤生长的作用,抑瘤作用机制可能是通过下调肿瘤组织Bcl-2表达活性、上调肿瘤组织Bax表达活性相关。
Objective To investigate the effect of musk onexpressions of human gastric cancer xenografts in nude mice and its mechanism Bcl-2 and Bax and the relevant mechanism. Methods Establish human gastric carcinoma xenograft model by injecting cell suspension in the subcutaneous. The 35 mice were randomly divided into Model group, 5-fluorouracil treatmentgroup, musk high dose group, middle dose group of musk and musk low-dose group.At the same time, they are given the drug based on the dosing regimen medication. When each medication 5dis used, a tumor size measure will be operated on nude mice and calculate the mean tumor volume in each group. Animals were sacrificed after treatment 21 d, stripping tumor, and caculating thetumor mass. Bcl-2 and Bax of tumor tissue expressions will be detected by immunohistochemistry. Results Compared with the model group, the administration of each group has different degreesof inhibition on the growth of human gastric cancer tumor, P <0.05. Compared with the model group, tumor mass is significantly lower in musk high, middle and low-dose group and 5-fluorouracilgroup, P <0.05.The Bcl-2 average gray value in both Musk high dose group and middle dose group musk were more significantly reduced than model group, P <0.05. The average surface density oneach treatment group, has little difference, compared with model control group, P> 0.05. In Musk high dose group and middle dose group musk, average gray value were significantly increased thanmodel group, P <0.05.Among each musk treatment group, the average surface density were significantly increased than the model group P <0.05. Conclusion Muskitself inhibit the growth ofsome tumor. Inhibitory mechanism may be related to tumor tissue by reducing 2 Bcl-expressing tumor activity,and increasing Bax expression activity.
Objective To investigate the effect of musk onexpressions of human gastric cancer xenografts in nude mice and its mechanism Bcl-2 and Bax and the relevant mechanism. Methods Establish human gastric carcinoma xenograft model by injecting cell suspension in the subcutaneous. The 35 mice were randomly divided into Model group, 5-fluorouracil treatmentgroup, musk high dose group, middle dose group of musk and musk low-dose group.At the same time, they are given the drug based on the dosing regimen medication. When each medication 5dis used, a tumor size measure will be operated on nude mice and calculate the mean tumor volume in each group. Animals were sacrificed after treatment 21 d, stripping tumor, and caculating thetumor mass. Bcl-2 and Bax of tumor tissue expressions will be detected by immunohistochemistry. Results Compared with the model group, the administration of each group has different degreesof inhibition on the growth of human gastric cancer tumor, P <0.05. Compared with the model group, tumor mass is significantly lower in musk high, middle and low-dose group and 5-fluorouracilgroup, P <0.05.The Bcl-2 average gray value in both Musk high dose group and middle dose group musk were more significantly reduced than model group, P <0.05. The average surface density oneach treatment group, has little difference, compared with model control group, P> 0.05. In Musk high dose group and middle dose group musk, average gray value were significantly increased thanmodel group, P <0.05.Among each musk treatment group, the average surface density were significantly increased than the model group P <0.05. Conclusion Muskitself inhibit the growth ofsome tumor. Inhibitory mechanism may be related to tumor tissue by reducing 2 Bcl-expressing tumor activity,and increasing Bax expression activity.
引文
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[4]何志军,陈先祥,等.移植瘤体积不同计测方法的比较[J].中国比较医学杂志,2009,19(9):47-50.
[5]李有富,李魏林.杨梅素对肝癌细胞Bel-7402裸鼠移植瘤的抑制作用[J].中国医药导报,2015,12(31):44-47.
[6]Dorrie J,Sapala K,Zunino SJ.Carnosol-induced apoptosis and Downer gulation of bcl-2 in blineage leukemia cells[J].Cancer Lett,2001,170(1):33-39.
[7]蒋本春,孙威.胃癌中HIF-1A、bcl-2和Bax的表达及临床意义[J].现代肿瘤医学,2010,18(10):1987-1991.
[8]刘瑾.白花蛇舌草和半枝莲配伍微粉对移植性小鼠肝癌肿瘤组织Bcl-2,Bax表达的影响[J].2011,17(21):227-230.
[9]孟照华,曾家修.皮下埋藏麝香对BALB/c纯系小鼠恶性肿瘤生长影响的实验研究[J].中国肿瘤临床,1998,25(11)834-836.