木犀草素在大鼠体内的代谢研究
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- 英文论文题名：Metabolism of Luteolin in Rats
- 论文作者：欧阳辉 ; 何明珍 ; 李军茂 ; 冯育林 ; 杨世林
- 英文论文作者：OUYANG Hui ; HE Ming-zhen ; LI Jun-mao ; FENG Yu-lin ; YANG Shi-lin ; JiangxiUniversity of Traditional Chinese Medicine ; State key laboratory of Innovative Drug and Energy-saving Pharmaceutical Equipments ; Nanchang Institute For Food and Drug Control
- 年：2016
- 作者机构：江西中医药大学;创新药物与高效节能降耗制药设备国家重点实验室;Nanchang Institute For Food and Drug Control;
- 论文关键词：木犀草素 ; 木犀草苷 ; 代谢产物 ; 黄酮类化合物
- 英文论文关键词：Luteolin ; Metabolites ; Flavonoids
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R285.5
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：9
- 文件大小：450k
- 原文格式：O
- 会议级别：全国
摘要
目的:研究木犀草素在大鼠体内的代谢转化。方法:应用UHPLC-Q-TOF-MS方法研究灌胃木犀草素后大鼠血浆、尿和粪中的代谢产物。结果:在灌胃木犀草素的大鼠血浆、尿、粪中共检测到25种代谢产物。结论:木犀草素在大鼠体内的主要代谢途径为氧化、甲基化、葡糖醛酸化和硫酸化。
Objective To investigate the metabolite profile of luteolin and luteoloside in rats.Methods Ultra high performance liquid chromatography combined with Triple TOF mass spectrometry(UHPLC-TOF/MS/MS) was used for the separation and analysis of luteolin and their metabolites.Results A total of 25 luteolin metabolites were detected in the plasma,urine and feces.Conclution The metabolic pathways of luteolin in rats included oxidation,demethylation,glucuronidation and sulfation.
Objective To investigate the metabolite profile of luteolin and luteoloside in rats.Methods Ultra high performance liquid chromatography combined with Triple TOF mass spectrometry(UHPLC-TOF/MS/MS) was used for the separation and analysis of luteolin and their metabolites.Results A total of 25 luteolin metabolites were detected in the plasma,urine and feces.Conclution The metabolic pathways of luteolin in rats included oxidation,demethylation,glucuronidation and sulfation.
引文
[1]Lin Y,Shi R,Wang X,et al.Luteolin,a flavonoid with potentials for cancer prevention and therapy[J].Curr Cancer Drug Targets,2008,8(7):634-646
[2]Xie F,Lang Q,Zhou M,et al.The dietary flavonoid luteolin inhibits Aurora B kinase activity and blocks proliferation of cancer cells[J].Eur J Pharm Sci,2012,46(5):388-396
[3]Choi A Y,Choi J H,Yoon H,et al.Luteolin induces apoptosis through endoplasmic reticulum stress and mitochondrial dysfunction in Neuro-2a mouse neuroblastoma cells[J].European journal of pharmacology,2011,668(1):115-126.
[4]Lee HZ,Yang WH,Bao BY,et al.Proteomic analysis reveals ATP-dependent steps and chaperones involvement in luteolin-induced lung cancer CH27 cell apoptosis[J].Eur J Pharmacol,2010,642(1-3):19-27
[5]Tang X,Wang H,Fan L,et al.Luteolin inhibits Nrf2 leading to negative regulation of the Nrf2/ARE pathway and sensitization of human lung carcinoma A549 cells to therapeutic drugs.Free Radic Biol Med,2011,50(11).1599-1609.
[6]Chen CY,Peng WH,Tsai KD,et al.Luteolin suppresses inflammation-associated gene expression by blocking NF-κB and AP-1 activation pathway in mouse alveolarmacrophages[J].Life Sci,2007,81(23-24):1602-1614.
[7]Lee JK,Kim SY,Kim YS,et al.Suppression of the TRIFdependent signaling pathway of Toll-like receptors by luteolin[J].Biochem Pharmacol,2009,77(8):1391-1400.
[8]Funakoshi-Tago M,Nakamura K,Tago K,et al.Anti-inflammatory activity of structurally related flavonoids,Apigenin,Luteolin and Fisetin[J].International immunopharmacology,2011,11(9):1150-1159.
[9]Li ZY,Zhou YM,Zhang N,et al.Evaluation of the anti-inflammatory activity of luteolin in experimental animalmodels[J].Planta Med,2007,73(3):221-226.
[10]W(o|¨)lfie U,Esser PR,Simon-Haarhaus B,et al.UVB-induced DNA damage,generation of reactive oxygen species,and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo.Free Radic BiolMed,2011,50(9):1081-1093
[11]Rooban BN,Sasikala V,Gayathri Devi V,et al.Prevention of selenite induced oxidative stress and cataractogenesis by luteolin isolated from Vitex negundo[J].Chem Biol Interact,2012,196(1-2):30-38
[12]Ashokkumar P,Sudhandiran G.Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis[J].Biomedicine&Pharmacotherapy,2008,62(9):590-597.
[13]Wang Q A.Natural food antistaling agent and its application[J].Jiangsu Food and Fermentation(江苏食品与发酵),2000,3:36-38.
[14]Chen H Q,Jin Z Y,Wang X J,et al.Luteolin protects dopaminergic neurons from inflammation-induced injury through inhibition of microglial activation[J].Neuroscience letters,2008,448(2):175-179.
[15]Kimata M,Inagaki N,Nagai H.Effects of luteolin and other flavonoids on IgE-mediated allergic reactions[J].Planta medica,2000,66(01):25-29.
[16]Jin J.Blood coagulant effect of the main chemical constituent of purslane speedwell(Veronica peregrina)[J].Chinese traditional and herbal drugs(中草药),1995,27(7):416-417.
[17]Li Y.UGT Metabolism of luteolin,orientin and isoorientin and BCRP regulation on the UGT metabolism[D].guangzhou:Southern Medical University(南方医科大学),2013.
[18]GIUSEPPE GALATI,MAJID Y.MORIDANI,et al.Peroxidative metabolism of apigenin and naringenin versus luteolin and quercetin:glutathione oxidation and conjugation[J].Original Contribution,2001,30(4):370-382.
[19]Liu C S,Song Y S,Zhang K J,et al.Gas chromatographic/mass spectrometric profiling of luteolin and its metabolites in rat urine and bile[J].Journal of pharmaceutical and biomedical analysis,1995,13(11):1409-1414.
[20]Tang L,Feng Q,Zhao J,et al.Involvement of UDP-glucuronosyltranferases and sulfotransferases in the liver and intestinal first-pass metabolism of seven flavones in C57 mice and humans in vitro[J].Food and chemical toxicology,2012,50(5):1460-1467.
[2]Xie F,Lang Q,Zhou M,et al.The dietary flavonoid luteolin inhibits Aurora B kinase activity and blocks proliferation of cancer cells[J].Eur J Pharm Sci,2012,46(5):388-396
[3]Choi A Y,Choi J H,Yoon H,et al.Luteolin induces apoptosis through endoplasmic reticulum stress and mitochondrial dysfunction in Neuro-2a mouse neuroblastoma cells[J].European journal of pharmacology,2011,668(1):115-126.
[4]Lee HZ,Yang WH,Bao BY,et al.Proteomic analysis reveals ATP-dependent steps and chaperones involvement in luteolin-induced lung cancer CH27 cell apoptosis[J].Eur J Pharmacol,2010,642(1-3):19-27
[5]Tang X,Wang H,Fan L,et al.Luteolin inhibits Nrf2 leading to negative regulation of the Nrf2/ARE pathway and sensitization of human lung carcinoma A549 cells to therapeutic drugs.Free Radic Biol Med,2011,50(11).1599-1609.
[6]Chen CY,Peng WH,Tsai KD,et al.Luteolin suppresses inflammation-associated gene expression by blocking NF-κB and AP-1 activation pathway in mouse alveolarmacrophages[J].Life Sci,2007,81(23-24):1602-1614.
[7]Lee JK,Kim SY,Kim YS,et al.Suppression of the TRIFdependent signaling pathway of Toll-like receptors by luteolin[J].Biochem Pharmacol,2009,77(8):1391-1400.
[8]Funakoshi-Tago M,Nakamura K,Tago K,et al.Anti-inflammatory activity of structurally related flavonoids,Apigenin,Luteolin and Fisetin[J].International immunopharmacology,2011,11(9):1150-1159.
[9]Li ZY,Zhou YM,Zhang N,et al.Evaluation of the anti-inflammatory activity of luteolin in experimental animalmodels[J].Planta Med,2007,73(3):221-226.
[10]W(o|¨)lfie U,Esser PR,Simon-Haarhaus B,et al.UVB-induced DNA damage,generation of reactive oxygen species,and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo.Free Radic BiolMed,2011,50(9):1081-1093
[11]Rooban BN,Sasikala V,Gayathri Devi V,et al.Prevention of selenite induced oxidative stress and cataractogenesis by luteolin isolated from Vitex negundo[J].Chem Biol Interact,2012,196(1-2):30-38
[12]Ashokkumar P,Sudhandiran G.Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis[J].Biomedicine&Pharmacotherapy,2008,62(9):590-597.
[13]Wang Q A.Natural food antistaling agent and its application[J].Jiangsu Food and Fermentation(江苏食品与发酵),2000,3:36-38.
[14]Chen H Q,Jin Z Y,Wang X J,et al.Luteolin protects dopaminergic neurons from inflammation-induced injury through inhibition of microglial activation[J].Neuroscience letters,2008,448(2):175-179.
[15]Kimata M,Inagaki N,Nagai H.Effects of luteolin and other flavonoids on IgE-mediated allergic reactions[J].Planta medica,2000,66(01):25-29.
[16]Jin J.Blood coagulant effect of the main chemical constituent of purslane speedwell(Veronica peregrina)[J].Chinese traditional and herbal drugs(中草药),1995,27(7):416-417.
[17]Li Y.UGT Metabolism of luteolin,orientin and isoorientin and BCRP regulation on the UGT metabolism[D].guangzhou:Southern Medical University(南方医科大学),2013.
[18]GIUSEPPE GALATI,MAJID Y.MORIDANI,et al.Peroxidative metabolism of apigenin and naringenin versus luteolin and quercetin:glutathione oxidation and conjugation[J].Original Contribution,2001,30(4):370-382.
[19]Liu C S,Song Y S,Zhang K J,et al.Gas chromatographic/mass spectrometric profiling of luteolin and its metabolites in rat urine and bile[J].Journal of pharmaceutical and biomedical analysis,1995,13(11):1409-1414.
[20]Tang L,Feng Q,Zhao J,et al.Involvement of UDP-glucuronosyltranferases and sulfotransferases in the liver and intestinal first-pass metabolism of seven flavones in C57 mice and humans in vitro[J].Food and chemical toxicology,2012,50(5):1460-1467.