硅胶基质高效液相色谱填料研究进展
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- 英文论文题名：Silica Based Stationary Phases for High Performance Liquid Chromatography
- 论文作者：赵贝贝 ; 张艳 ; 唐涛 ; 王风云 ; 张维冰 ; 李彤
- 英文论文作者：Zhao Beibei ; Zhang Yan ; Tang Tao ; Wang Fengyun ; Zhang Weibing ; Li Tong ; Chemical Engineering Institute ; Nanjing University of Science and Technology ; Dalian Elite Analytical Instruments Co. ; Ltd. ; Shanghai Key Laboratory of Functional Materials Chemistry ; East China University of Science and Technology
- 年：2015
- 作者机构：南京理工大学工业化学研究所;大连依利特分析仪器有限公司;华东理工大学上海市功能性材料化学重点实验室;
- 论文关键词：高效液相色谱 ; 硅胶 ; 填料
- 英文论文关键词：high performance liquid chromatography(HPLC) ; silica ; packing material
- 会议召开时间：2015-09-22
- 会议录名称：2015年全国无机硅化物行业年会暨新常态行业发展研讨会论文集
- 语种：中文
- 分类号：O652.63
- 学会代码：WJYG
- 会议名称：2015年全国无机硅化物行业年会暨新常态行业发展研讨会
- 会议地点：中国湖北宜昌
- 主办单位：中国无机盐工业协会无机硅化物分会
- 学会名称：中国无机盐工业协会无机硅化物分会
- 页数：8
- 文件大小：1790k
- 原文格式：O
- 会议级别：全国
摘要
高效液相色谱(HPLC)不仅是一种有效的分析分离手段,也是一种重要的高效制备分离技术。色谱柱是HPLC系统的核心,不同性能的填料是HPLC广泛应用的基础。硅胶是开发最早、研究最为深入、应用最为广泛的HPLC固定相基质,其制备方法主要有喷雾干燥法、溶胶-凝胶法、聚合诱导胶体凝聚法及模板法等。近年来,亚2μm小粒径硅胶、核-壳型硅胶、双孔径硅胶、介孔性硅胶、有机杂化硅胶等新型硅胶应用于HPLC并取得了色谱分离技术的飞速发展,例如基于亚2μm填料的超高压液相色谱技术、基于核-壳型填料的快速分离技术、基于杂化硅胶填料的高温液相色谱技术等。硅胶经表面化学键合、聚合物包覆等有机改性可制得先进的大分子限进填料、温敏性填料、手性填料等,大大扩展了HPLC的应用范围。本文对液相色谱用硅胶的制备方法、改性与修饰方法以及硅胶基质固定相的评价方法加以系统综述,概述了新型硅胶在HPLC中的应用进展,并对硅胶基质填料的发展方向与应用前景进行了展望。
High performance liquid chromatography is not only a useful analytical technique,but also aneffective preparation method.The availability of a variety of stationary phases for column has been a key factor inthe development of HPLC as a major scientific tool.With the most desirable compromise of properties that provide for effective and reproducible separations,silica has been the most widely used HPLC packing material.The silica microspheres are synthesized by various methods,including spray drying,sol- gel,polymerization induced colloidaggregation and templating methods.In recent years,atypical types of silica are prepared and applied in HPLC,such as sub-2μm silica particles,superficially porous silica particles,bimodal silica particles,mesoporous silica particles,organic/silica hybrid particles,etc.As a result,unique separation properties that enlarge the capabilities of HPLC methods have been achieved,such as ultrahigh- pressure liquid chromatography based on sub-2μm silica particles,fast liquid chromatography based on superficially porous silica particles,high temperature liquid chromatography based on organic / silicahybrid particles.Moreover,novel stationary phase can be obtained by chemical bonding or polymer modification of silica surface,such as chiral stationary phase,temperature- responsivestationary phase and restricted access materials.In this paper,the preparation methods and modification modes of silica particles are introduced,as well as the characterization methods of HPLC stationary phase.The applicationsof silica packing material in HPLC and its developing trends are also outlined.
High performance liquid chromatography is not only a useful analytical technique,but also aneffective preparation method.The availability of a variety of stationary phases for column has been a key factor inthe development of HPLC as a major scientific tool.With the most desirable compromise of properties that provide for effective and reproducible separations,silica has been the most widely used HPLC packing material.The silica microspheres are synthesized by various methods,including spray drying,sol- gel,polymerization induced colloidaggregation and templating methods.In recent years,atypical types of silica are prepared and applied in HPLC,such as sub-2μm silica particles,superficially porous silica particles,bimodal silica particles,mesoporous silica particles,organic/silica hybrid particles,etc.As a result,unique separation properties that enlarge the capabilities of HPLC methods have been achieved,such as ultrahigh- pressure liquid chromatography based on sub-2μm silica particles,fast liquid chromatography based on superficially porous silica particles,high temperature liquid chromatography based on organic / silicahybrid particles.Moreover,novel stationary phase can be obtained by chemical bonding or polymer modification of silica surface,such as chiral stationary phase,temperature- responsivestationary phase and restricted access materials.In this paper,the preparation methods and modification modes of silica particles are introduced,as well as the characterization methods of HPLC stationary phase.The applicationsof silica packing material in HPLC and its developing trends are also outlined.
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