Factor H specifically capture novel Factor H-binding proteins of Streptococcus suis, and contribute to the adherence and invasion of epithelial cells and promote mouse infection
详细信息 查看官网全文
- 英文论文题名：Factor H specifically capture novel Factor H-binding proteins of Streptococcus suis, and contribute to the adherence and invasion of epithelial cells and promote mouse infection
- 论文作者：Li Quan ; Zhang Wei
- 英文论文作者：Li Quan ; Zhang Wei ; Key Lab of Animal Bacteriology ; OIE Reference Lab for Swine Streptococcosis ; College of Veterinary Medicine ; Ministry of Agriculture ; Nanjing Agricultural University
- 年：2016
- 作者机构：Key Lab of Animal Bacteriology,OIE Reference Lab for Swine Streptococcosis,College of Veterinary Medicine,Ministry of Agriculture,Nanjing Agricultural University;
- 英文论文关键词：Streptococcus suis serotype 2 ; factor H-binding proteins ; adherence ; invasion ; interaction ; virulenc
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：S852.61
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1334k
- 原文格式：D
- 会议级别：全国
摘要
Background: Factor H(FH), a regulatory protein of the complement system, can bind specifically to factor H-binding proteins(FHBPs) of Streptococcus suis serotype 2(SS2), which contribute to evasion of host innate immune defenses. In the present study, we aimed to identify novel FHBPs and characterize the biological functions of FH in SS2 pathogenesis.Method: Here, a method that combined proteomics and Far-western blotting was developed to identify the surface FHBPs of SS2. we sought to characterize whether SS2 interact specifically with mouse FH and evaluated whether FH could influence the adhesion and invasion of SS2 to HEp-2 cells. To evaluate the roles of FH in vivo, we infected a specific pathogen free mouse model to evaluate the pathogenesis of SS2 in the presence of human FH.Results: With this method, fourteen potential novel FHBPs were identified among SS2 surface proteins. We selected eight newly identied proteins and further confirmed their binding activity to FH. The binding of SS2 to immobilized FH decreased dramatically after pre-incubation with anti-FHBPs polyclonal antibodies. We showed for the first time that SS2 also interact specifically with mouse FH. Furthermore, we found that FH play an important role in adherence and invasion of SS2 to HEp-2 cells. Additionally, using a mouse model of intraperitoneal challenge, we confirmed that SS2 preincubated with FH enhanced bacteremia and brain invasion, compared with SS2 not pretreated with FH.Conclusion: Taken together, this study provides a useful method to characterize the host-bacteria interactions. These results firstly indicated that binding of FH to the cell surface improved the adherence and invasion of SS2 to HEp-2 cells, promoted SS2 to resist killing and enhanced virulence.
Background: Factor H(FH), a regulatory protein of the complement system, can bind specifically to factor H-binding proteins(FHBPs) of Streptococcus suis serotype 2(SS2), which contribute to evasion of host innate immune defenses. In the present study, we aimed to identify novel FHBPs and characterize the biological functions of FH in SS2 pathogenesis.Method: Here, a method that combined proteomics and Far-western blotting was developed to identify the surface FHBPs of SS2. we sought to characterize whether SS2 interact specifically with mouse FH and evaluated whether FH could influence the adhesion and invasion of SS2 to HEp-2 cells. To evaluate the roles of FH in vivo, we infected a specific pathogen free mouse model to evaluate the pathogenesis of SS2 in the presence of human FH.Results: With this method, fourteen potential novel FHBPs were identified among SS2 surface proteins. We selected eight newly identied proteins and further confirmed their binding activity to FH. The binding of SS2 to immobilized FH decreased dramatically after pre-incubation with anti-FHBPs polyclonal antibodies. We showed for the first time that SS2 also interact specifically with mouse FH. Furthermore, we found that FH play an important role in adherence and invasion of SS2 to HEp-2 cells. Additionally, using a mouse model of intraperitoneal challenge, we confirmed that SS2 preincubated with FH enhanced bacteremia and brain invasion, compared with SS2 not pretreated with FH.Conclusion: Taken together, this study provides a useful method to characterize the host-bacteria interactions. These results firstly indicated that binding of FH to the cell surface improved the adherence and invasion of SS2 to HEp-2 cells, promoted SS2 to resist killing and enhanced virulence.
Background: Factor H(FH), a regulatory protein of the complement system, can bind specifically to factor H-binding proteins(FHBPs) of Streptococcus suis serotype 2(SS2), which contribute to evasion of host innate immune defenses. In the present study, we aimed to identify novel FHBPs and characterize the biological functions of FH in SS2 pathogenesis.Method: Here, a method that combined proteomics and Far-western blotting was developed to identify the surface FHBPs of SS2. we sought to characterize whether SS2 interact specifically with mouse FH and evaluated whether FH could influence the adhesion and invasion of SS2 to HEp-2 cells. To evaluate the roles of FH in vivo, we infected a specific pathogen free mouse model to evaluate the pathogenesis of SS2 in the presence of human FH.Results: With this method, fourteen potential novel FHBPs were identified among SS2 surface proteins. We selected eight newly identied proteins and further confirmed their binding activity to FH. The binding of SS2 to immobilized FH decreased dramatically after pre-incubation with anti-FHBPs polyclonal antibodies. We showed for the first time that SS2 also interact specifically with mouse FH. Furthermore, we found that FH play an important role in adherence and invasion of SS2 to HEp-2 cells. Additionally, using a mouse model of intraperitoneal challenge, we confirmed that SS2 preincubated with FH enhanced bacteremia and brain invasion, compared with SS2 not pretreated with FH.Conclusion: Taken together, this study provides a useful method to characterize the host-bacteria interactions. These results firstly indicated that binding of FH to the cell surface improved the adherence and invasion of SS2 to HEp-2 cells, promoted SS2 to resist killing and enhanced virulence.
引文