The Nramp6 Metal Transporter from Sedum alfredii Contributes to Cadimium Toxicity
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- 英文论文题名：The Nramp6 Metal Transporter from Sedum alfredii Contributes to Cadimium Toxicity
- 论文作者：Shuangshuang Chen ; Xiaojiao Han ; Jie Fang ; Mingying Liu ; Jian Sang ; Jing Jiang ; Renying Zhuo
- 英文论文作者：Shuangshuang Chen ; Xiaojiao Han ; Jie Fang ; Mingying Liu ; Jian Sang ; Jing Jiang ; Renying Zhuo ; State Key Laboratory of Forest Genetics and Breeding ; Key Lab of Tree Genomics ; The Research Institute of Subtropical of Forestry ; Chinese Academy of Forestry ; Chemical Biology Center ; Lishui Institute of Agricultural Sciences
- 年：2016
- 作者机构：State Key Laboratory of Forest Genetics and Breeding;Key Lab of Tree Genomics,The Research Institute of Subtropical of Forestry,Chinese Academy of Forestry;Chemical Biology Center,Lishui Institute of Agricultural Sciences;
- 英文论文关键词：Nramp ; Sedum alfredii ; Cadmium stress ; overexpression
- 会议召开时间：2016-09-01
- 会议录名称：全国农业生物化学与分子生物学第十五届学术研讨会会议文集
- 英文会议录名称：The Special Edition of the 15th National Symposium of Agricultural Biochemistry and Molecular Biology
- 语种：英文
- 分类号：X171.5;Q943.2
- 学会代码：IGSS
- 会议名称：全国农业生物化学与分子生物学第十五届学术研讨会
- 会议地点：中国甘肃兰州
- 主办单位：中国生物化学与分子生物学会农业分会
- 学会名称：中国生物化学与分子生物学会
- 页数：18
- 文件大小：6510k
- 原文格式：O
- 会议级别：全国
摘要
Plant natural resistance-associated macrophage protein(Nramp) families play an important role in tolerance of heavy metal stress.In this study,we cloned and identified the SaNramp6 gene from the heavy metal-hyperaccumulating plant Sedum alfredii and analyzed its function in transgenic Arabidopsis thaliana.The 1638-bp cDNA for SaNramp6 encodes 545 amino acids.SaNramp6 was strongly expressed in roots and leaves,and its transcription of root increased in response to Cd stress,with expression reaching a maximum in roots 1 week after treatment.SaNramp6 fused to green fluorescent protein was localized to the plasma membrane in both Nicotiana benthamiana lower leaf epidermal cells and onion(Allium cepa) epidermal cells.Transgenic A.thaliana expressing SaNramp6 exhibited higher Cd toxicity as compared with the control,with higher cadmium concentration,MDA content,electrolyte leakage and antioxidant enzyme activity,as well as lower chlorophyll content under Cd stress.DAB and NBT staining also indicated that the transgenic plants accumulate more reactive oxygen species compared to the control when exposed to CdCl_2.Overall,these results suggest that overexpression of SaNramp6 plays a significant role in improving the cadmium accumulation,and the gene may be useful for the biotechnological development of transgenic plants to phytoremediate soil contaminated with heavy metals.
Plant natural resistance-associated macrophage protein(Nramp) families play an important role in tolerance of heavy metal stress.In this study,we cloned and identified the SaNramp6 gene from the heavy metal-hyperaccumulating plant Sedum alfredii and analyzed its function in transgenic Arabidopsis thaliana.The 1638-bp cDNA for SaNramp6 encodes 545 amino acids.SaNramp6 was strongly expressed in roots and leaves,and its transcription of root increased in response to Cd stress,with expression reaching a maximum in roots 1 week after treatment.SaNramp6 fused to green fluorescent protein was localized to the plasma membrane in both Nicotiana benthamiana lower leaf epidermal cells and onion(Allium cepa) epidermal cells.Transgenic A.thaliana expressing SaNramp6 exhibited higher Cd toxicity as compared with the control,with higher cadmium concentration,MDA content,electrolyte leakage and antioxidant enzyme activity,as well as lower chlorophyll content under Cd stress.DAB and NBT staining also indicated that the transgenic plants accumulate more reactive oxygen species compared to the control when exposed to CdCl_2.Overall,these results suggest that overexpression of SaNramp6 plays a significant role in improving the cadmium accumulation,and the gene may be useful for the biotechnological development of transgenic plants to phytoremediate soil contaminated with heavy metals.
Plant natural resistance-associated macrophage protein(Nramp) families play an important role in tolerance of heavy metal stress.In this study,we cloned and identified the SaNramp6 gene from the heavy metal-hyperaccumulating plant Sedum alfredii and analyzed its function in transgenic Arabidopsis thaliana.The 1638-bp cDNA for SaNramp6 encodes 545 amino acids.SaNramp6 was strongly expressed in roots and leaves,and its transcription of root increased in response to Cd stress,with expression reaching a maximum in roots 1 week after treatment.SaNramp6 fused to green fluorescent protein was localized to the plasma membrane in both Nicotiana benthamiana lower leaf epidermal cells and onion(Allium cepa) epidermal cells.Transgenic A.thaliana expressing SaNramp6 exhibited higher Cd toxicity as compared with the control,with higher cadmium concentration,MDA content,electrolyte leakage and antioxidant enzyme activity,as well as lower chlorophyll content under Cd stress.DAB and NBT staining also indicated that the transgenic plants accumulate more reactive oxygen species compared to the control when exposed to CdCl_2.Overall,these results suggest that overexpression of SaNramp6 plays a significant role in improving the cadmium accumulation,and the gene may be useful for the biotechnological development of transgenic plants to phytoremediate soil contaminated with heavy metals.
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