Functional characterization of a Glycine soja Ca~(2+)ATPase in saltalkaline stress responses
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- 英文论文题名：Functional characterization of a Glycine soja Ca~(2+)ATPase in saltalkaline stress responses
- 论文作者：Mingzhe Sun ; Bowei Jia ; Na Cui ; Yidong Wen ; Huizi Duanmu ; Yanming Zhu ; Xiaoli Sun
- 英文论文作者：Mingzhe Sun ; Bowei Jia ; Na Cui ; Yidong Wen ; Huizi Duanmu ; Yanming Zhu ; Xiaoli Sun ; Crop Stress Molecular Biology Laboratory ; Heilongjiang Bayi Agricultural University
- 年：2016
- 作者机构：Crop Stress Molecular Biology Laboratory,Heilongjiang Bayi Agricultural University;
- 英文论文关键词：Carbonate alkaline stress ; salt stress ; Ca2+ATPase ; Glycine soja ; alfalfa
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S565.1;Q945.78
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
It is widely accepted that Ca~(2+)ATPase family proteins play important roles in plant environmental stress responses. However,up to now,most researches are limited in the reference plants Arabidopsis and rice. The function of Ca~(2+)ATPases from non-reference plants was rarely reported,especially its regulatory role in carbonate alkaline stress responses. Hence,in this study,we identified the P-type II Ca~(2+)ATPase family genes in soybean genome,determined their chromosomal location and gene architecture,and analyzed their amino acid sequence and evolutionary relationship. Based on above results,we pointed out the existence of gene duplication for soybean Ca~(2+)ATPases. Then,we investigated the expression profiles of the ACA subfamily genes in wild soybean(Glycine soja) under carbonate alkaline stress,and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses,as evidenced by lower levels of membrane permeability and MDA content,and higher levels of SOD activity,proline concentration and chlorophyll content under stress conditions. Taken together,for the first time,we reported a Ptype II Ca~(2+)ATPase from wild soybean,GsACA1,which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.
It is widely accepted that Ca~(2+)ATPase family proteins play important roles in plant environmental stress responses. However,up to now,most researches are limited in the reference plants Arabidopsis and rice. The function of Ca~(2+)ATPases from non-reference plants was rarely reported,especially its regulatory role in carbonate alkaline stress responses. Hence,in this study,we identified the P-type II Ca~(2+)ATPase family genes in soybean genome,determined their chromosomal location and gene architecture,and analyzed their amino acid sequence and evolutionary relationship. Based on above results,we pointed out the existence of gene duplication for soybean Ca~(2+)ATPases. Then,we investigated the expression profiles of the ACA subfamily genes in wild soybean(Glycine soja) under carbonate alkaline stress,and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses,as evidenced by lower levels of membrane permeability and MDA content,and higher levels of SOD activity,proline concentration and chlorophyll content under stress conditions. Taken together,for the first time,we reported a Ptype II Ca~(2+)ATPase from wild soybean,GsACA1,which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.
It is widely accepted that Ca~(2+)ATPase family proteins play important roles in plant environmental stress responses. However,up to now,most researches are limited in the reference plants Arabidopsis and rice. The function of Ca~(2+)ATPases from non-reference plants was rarely reported,especially its regulatory role in carbonate alkaline stress responses. Hence,in this study,we identified the P-type II Ca~(2+)ATPase family genes in soybean genome,determined their chromosomal location and gene architecture,and analyzed their amino acid sequence and evolutionary relationship. Based on above results,we pointed out the existence of gene duplication for soybean Ca~(2+)ATPases. Then,we investigated the expression profiles of the ACA subfamily genes in wild soybean(Glycine soja) under carbonate alkaline stress,and functionally characterized one representative gene GsACA1 by using transgenic alfalfa. Our results suggested that GsACA1 overexpression in alfalfa obviously increased plant tolerance to both carbonate alkaline and neutral salt stresses,as evidenced by lower levels of membrane permeability and MDA content,and higher levels of SOD activity,proline concentration and chlorophyll content under stress conditions. Taken together,for the first time,we reported a Ptype II Ca~(2+)ATPase from wild soybean,GsACA1,which could positively regulate plant tolerance to both carbonate alkaline and neutral salt stresses.
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