母猪乳腺发育及乳脂合成与分泌的分子路径研究进展
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摘要
母猪的泌乳力和乳汁质量很大程度上决定了所哺育仔猪的生长速度、成活率,间接影响仔猪断奶后的生长及后期生长。母猪泌乳力的大小和乳汁质量与其乳腺组织的生长发育密切相关。在母猪的繁殖周期中,随着从妊娠到泌乳的进行,乳腺腺泡组织逐渐替代脂肪组织成为泌乳组织,其组织重量与体积、DNA含量、蛋白含量等也发生了显著变化。同时乳腺中与乳成分合成的底物转运及其他生物合成功能基因发生上调,与乳腺上皮细胞增殖相关的功能基因则下调。乳脂是母猪乳汁的重要组成成分,常乳中的乳脂含量显著高于初乳,这与乳脂合成与分泌相关功能基因在母猪泌乳期乳腺组织中高表达有关。本文围绕母猪乳腺组织在妊娠-泌乳周期生长发育过程中组织和成分变化、相关功能基因变化规律及乳脂合成与分泌相关基因表达特点进行了综述。
引文
[1]陈宝良.母猪乳腺葡萄糖转运以及乳糖合成机制的研究.硕士学位论文.广州:华南农业大学,2012.
[2]候丹熹.母猪乳腺凋亡基因表达差异研究及L-肉碱对乳腺上皮细胞增殖、凋亡的影响.硕士学位论文.广州:华南农业大学,2013.
[3]舒丹平.母猪泌乳功能基因的筛选及乳腺氨基酸转运的机理研究.博士学位论文.广州:华南农业大学,2012.
[4]苏志熙,董新姣,张兵,等.泌乳前后家猪乳腺基因表达谱的构建以及不同猪种和不同发育时期差异表达基因的确定.中国科学C辑:生命科学,2005,35:462-471.
[5]Barber M C,Vallance A J,Kennedy H T,et al..Induction of transcripts derived from promoter III of the acetylCoA carboxylase-alpha gene in mammary gland is associated with recruitment of SREBP-1 to a region of the proximal promoter defined by a DNase I hypersensitive site.Biochem J,2003,375:489-501.
[6]Bionaz M,Loor J J.ACSL1,AGPAT6,FABP3,LPIN1,and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation.J Nutr,2008,138:1019-1024.
[7]Bionaz M,Loor J J.Gene networks driving bovine milk fat synthesis during the lactation cycle.BMC Genomics,2008,9:366.
[8]Boyd D R,Kensinger R S.Metabolic precursors for milk synthesis.In.Verstegen M WA,Moughan P J,Scharma J W(Eds),The lactating sow.Wageningen Press,Wageningen,1998,71-95.
[9]Capuco A V,Wood D L,Baldwin R,et al..Mammary cell number,proliferation,and apoptosis during a bovine lactation:relation to milk production and effect of bST.J Dairy Sci,2001,84:2177-2187.
[10]Csapo J,Martin T G,Csapo-Kiss Z S,et al..Protein,fats,vitamin and mineral concentrations in porcine colostrum and milk from parturition to 60 days.Int Dairy J,1996,6:881-902.
[11]Green M H,Dohner E L,Green J B.Influence of dietary fat and cholesterol on milk lipids and on cholesterol metabolism in the rat.J Nutr,1981,111,276-286.
[12]de Jong H,Neal A C,Coleman R A,et al..Ontogeny of mRNA expression and activity of long-chain acyl-CoA synthetase(ACSL)isoforms in Mus musculus heart.Biochim Biophys Acta,2007,1771:75-82.
[13]Hacker R R,Hill D L.Nucleic acid content of mammary glands of virgin and pregnant gilts.J Dairy Sci,1972,55:1295-1299.
[14]Hurley W L.Mammary gland growth in the lactating sow.Livest Produc Sci,2001,70:149-157.
[15]Ji F,Hurley W L,Kim S W.Characterization of mammary gland development in pregnant gilts.J Anim Sci,2006,84:579-587.
[16]Kadegowda A K,Bionaz M,Piperova L S,et al..Peroxisome proliferator-activated receptor-gamma activation and long-chain fatty acids alter lipogenic gene networks in bovine mammary epithelial cells to various extents.J Dairy Sci,2009,92:4276-4289.
[17]Kensinger R S,Collier R J,Bazer F W,et al..Nucleic acid,metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.J Anim Sci,1982,54:1297-1308.
[18]Kensinger R S,Collier R J,Bazer F W,et al..Nucleic acid,metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.J Anim Sci,1982,54:1297-1308.
[19]Kim S W,Hurley W L,Han I K,et al..Changes in tissue composition associated with mammary gland growth during lactation.J Anim Sci,1999,77:2510-2516.
[20]Klobasa F,Werhahn E,Butler J E.Composition of sow milk during lactation.J Anim Sci,1987,64:1458-1466.
[21]Knight C H,Docherty A H,Peaker M.Milk yield in rats in relation to activity and size of the mammary secretory cell population.J Dairy Res,1984,51:29-36.
[22]Laws J,Amusquivar E,Laws A,et al,Dodds P F,Clarke L.Supplementation of sow diets with oil during gestation:Sow body condition,milk yield and milk composition.Livest Sci,2009,123:88-96.
[23]Lv Y,Guan W,Qiao H,et al..Veterinary medicine and omics(veterinomics):metabolic transition of milk triacylglycerol synthesis in sows from late pregnancy to lactation.OMICS,2015,19:602-616.
[24]Ma L,Corl B A.Transcriptional regulation of lipid synthesis in bovine mammary epithelial cells by sterol regulatory element binding protein-1.J Dairy Sci,2012,95:3743-3755.
[25]Manjarin R,Steibel J P,Zamora V,et al,Ernst C W,Weber P S,Taylor N P,Trottier N L.Transcript abundance of amino acid transporters,β-casein,andα-crlactalbumin in mammary tissue of perparturient,lactating,and postweaned sows.J Dairy Sci,2011,94:3467-3476.
[26]McFadden J W,Corl B A.Activation of liver X receptor(LXR)enhances de novo fatty acid synthesis in bovine mammary epithelial cells.J Dairy Sci,2010,93:4651-4658.
[27]Mohammad M A,Haymond M W.Regulation of lipid synthesis genes and milk fat production in human mammary epithelial cells during secretory activation.Am J Physiol Endocrinol Metab,2013,305:E700-716.
[28]Motyl T,Gajkowska B,Wojewodzka U,et al..Expression of apoptosis-related proteins in involuting mammary gland of sow.Comp Biochem Physiol B Biochem Mol Biol,2001,128:635-646.
[29]Neville M C,McFadden T B,Forsyth I.Hormonal regulation of mammary differentiation and milk secretion.J Mammary Gland Biol Neoplasia,2002,7:49-66.
[30]Neville M C,Morton J,Umemura S.Lactogenesis.The Transition from Pregnancy to Lactation.Pediatr Clin North Am.2001,48:35-52.
[31]Oppi-Williams C,Suagee J K,Corl B A.Regulation of lipid synthesis by liver X receptorαand sterol regulatory element-binding protein 1 in mammary epithelial cells.J Dairy Sci,2013,96:112-121.
[32]Pluske J R,Dong G Z.Factors influencing the utilization of colostrum and milk.In:Verstegen M W A,Moughan P J,Scharma J W(Eds),The lactating sow.Wageningen Press,Wageningen,1998,45-70.
[33]Richert B T,Goodband R D,Tokach M D,et al..In vitro oxidation of branched chain amino acids by porcine mammary tissue.Nutr Res.1998,18:833-840.
[34]Ross A C,Davila M E,Cleary M P.Fatty acids and retinyl esters of rat milk:effects of diet and duration of lactation.J Nutr,1985,115:1488-1497.
[35]Scow R O,Chernick S S,Fleck T R.Lipoprotein lipase and uptake of triacylglycerol,cholesterol and phosphatidylcholine from chylomicrons by mammary and adipose tissue of lactating rats in vivo.Biochim Biophys Acta,1977,487:297-306.
[36]Shu D P,Chen B L,Hong J,et al..Global transcriptional profiling in porcine mammary glands from late pregnancy to peak lactation.OMICS,2012,16:123-137.
[37]S(?)rensen M T,Sejrsen K,Purup S.Mammary gland development in gilts.Livest Produc Sci,2002,75:143-148.
[38]Stefanon B,Colitti M,Gabai G,et al..Mammary apoptosis and lactation persistency in dairy animals.J Dairy Res,2002,69:37-52.
[39]Theil P K,Labouriau R,Sejrsen K,et al..Expression of genes involved in regulation of cell turnover during milk stasis and lactation rescue in sow mammary glands.J Anim Sci,2005,83:2349-2356.
[40]Theil P K,Sejrsen K,Hurley W L,et al..Role of suckling in regulating cell turnover and onset and maintenance of lactation in individual mammary glands of sows.J Anim Sci,2006,84:1691-1698.
[41]Trottier N L,Shipley C F,Easter R A.Plasma amino acid uptake by the mammary gland of the lactating sow.J Anim Sci,1997,75:1266-1278.
[42]Winn R J,Baker M D,Merle C A,et al..Individual and combined effects of relaxin,estrogen,and progesterone in ovariectomized gilts.II.Effects on mammary development.Endocrinology,1994,135:1250-1255.
[2]候丹熹.母猪乳腺凋亡基因表达差异研究及L-肉碱对乳腺上皮细胞增殖、凋亡的影响.硕士学位论文.广州:华南农业大学,2013.
[3]舒丹平.母猪泌乳功能基因的筛选及乳腺氨基酸转运的机理研究.博士学位论文.广州:华南农业大学,2012.
[4]苏志熙,董新姣,张兵,等.泌乳前后家猪乳腺基因表达谱的构建以及不同猪种和不同发育时期差异表达基因的确定.中国科学C辑:生命科学,2005,35:462-471.
[5]Barber M C,Vallance A J,Kennedy H T,et al..Induction of transcripts derived from promoter III of the acetylCoA carboxylase-alpha gene in mammary gland is associated with recruitment of SREBP-1 to a region of the proximal promoter defined by a DNase I hypersensitive site.Biochem J,2003,375:489-501.
[6]Bionaz M,Loor J J.ACSL1,AGPAT6,FABP3,LPIN1,and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation.J Nutr,2008,138:1019-1024.
[7]Bionaz M,Loor J J.Gene networks driving bovine milk fat synthesis during the lactation cycle.BMC Genomics,2008,9:366.
[8]Boyd D R,Kensinger R S.Metabolic precursors for milk synthesis.In.Verstegen M WA,Moughan P J,Scharma J W(Eds),The lactating sow.Wageningen Press,Wageningen,1998,71-95.
[9]Capuco A V,Wood D L,Baldwin R,et al..Mammary cell number,proliferation,and apoptosis during a bovine lactation:relation to milk production and effect of bST.J Dairy Sci,2001,84:2177-2187.
[10]Csapo J,Martin T G,Csapo-Kiss Z S,et al..Protein,fats,vitamin and mineral concentrations in porcine colostrum and milk from parturition to 60 days.Int Dairy J,1996,6:881-902.
[11]Green M H,Dohner E L,Green J B.Influence of dietary fat and cholesterol on milk lipids and on cholesterol metabolism in the rat.J Nutr,1981,111,276-286.
[12]de Jong H,Neal A C,Coleman R A,et al..Ontogeny of mRNA expression and activity of long-chain acyl-CoA synthetase(ACSL)isoforms in Mus musculus heart.Biochim Biophys Acta,2007,1771:75-82.
[13]Hacker R R,Hill D L.Nucleic acid content of mammary glands of virgin and pregnant gilts.J Dairy Sci,1972,55:1295-1299.
[14]Hurley W L.Mammary gland growth in the lactating sow.Livest Produc Sci,2001,70:149-157.
[15]Ji F,Hurley W L,Kim S W.Characterization of mammary gland development in pregnant gilts.J Anim Sci,2006,84:579-587.
[16]Kadegowda A K,Bionaz M,Piperova L S,et al..Peroxisome proliferator-activated receptor-gamma activation and long-chain fatty acids alter lipogenic gene networks in bovine mammary epithelial cells to various extents.J Dairy Sci,2009,92:4276-4289.
[17]Kensinger R S,Collier R J,Bazer F W,et al..Nucleic acid,metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.J Anim Sci,1982,54:1297-1308.
[18]Kensinger R S,Collier R J,Bazer F W,et al..Nucleic acid,metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis.J Anim Sci,1982,54:1297-1308.
[19]Kim S W,Hurley W L,Han I K,et al..Changes in tissue composition associated with mammary gland growth during lactation.J Anim Sci,1999,77:2510-2516.
[20]Klobasa F,Werhahn E,Butler J E.Composition of sow milk during lactation.J Anim Sci,1987,64:1458-1466.
[21]Knight C H,Docherty A H,Peaker M.Milk yield in rats in relation to activity and size of the mammary secretory cell population.J Dairy Res,1984,51:29-36.
[22]Laws J,Amusquivar E,Laws A,et al,Dodds P F,Clarke L.Supplementation of sow diets with oil during gestation:Sow body condition,milk yield and milk composition.Livest Sci,2009,123:88-96.
[23]Lv Y,Guan W,Qiao H,et al..Veterinary medicine and omics(veterinomics):metabolic transition of milk triacylglycerol synthesis in sows from late pregnancy to lactation.OMICS,2015,19:602-616.
[24]Ma L,Corl B A.Transcriptional regulation of lipid synthesis in bovine mammary epithelial cells by sterol regulatory element binding protein-1.J Dairy Sci,2012,95:3743-3755.
[25]Manjarin R,Steibel J P,Zamora V,et al,Ernst C W,Weber P S,Taylor N P,Trottier N L.Transcript abundance of amino acid transporters,β-casein,andα-crlactalbumin in mammary tissue of perparturient,lactating,and postweaned sows.J Dairy Sci,2011,94:3467-3476.
[26]McFadden J W,Corl B A.Activation of liver X receptor(LXR)enhances de novo fatty acid synthesis in bovine mammary epithelial cells.J Dairy Sci,2010,93:4651-4658.
[27]Mohammad M A,Haymond M W.Regulation of lipid synthesis genes and milk fat production in human mammary epithelial cells during secretory activation.Am J Physiol Endocrinol Metab,2013,305:E700-716.
[28]Motyl T,Gajkowska B,Wojewodzka U,et al..Expression of apoptosis-related proteins in involuting mammary gland of sow.Comp Biochem Physiol B Biochem Mol Biol,2001,128:635-646.
[29]Neville M C,McFadden T B,Forsyth I.Hormonal regulation of mammary differentiation and milk secretion.J Mammary Gland Biol Neoplasia,2002,7:49-66.
[30]Neville M C,Morton J,Umemura S.Lactogenesis.The Transition from Pregnancy to Lactation.Pediatr Clin North Am.2001,48:35-52.
[31]Oppi-Williams C,Suagee J K,Corl B A.Regulation of lipid synthesis by liver X receptorαand sterol regulatory element-binding protein 1 in mammary epithelial cells.J Dairy Sci,2013,96:112-121.
[32]Pluske J R,Dong G Z.Factors influencing the utilization of colostrum and milk.In:Verstegen M W A,Moughan P J,Scharma J W(Eds),The lactating sow.Wageningen Press,Wageningen,1998,45-70.
[33]Richert B T,Goodband R D,Tokach M D,et al..In vitro oxidation of branched chain amino acids by porcine mammary tissue.Nutr Res.1998,18:833-840.
[34]Ross A C,Davila M E,Cleary M P.Fatty acids and retinyl esters of rat milk:effects of diet and duration of lactation.J Nutr,1985,115:1488-1497.
[35]Scow R O,Chernick S S,Fleck T R.Lipoprotein lipase and uptake of triacylglycerol,cholesterol and phosphatidylcholine from chylomicrons by mammary and adipose tissue of lactating rats in vivo.Biochim Biophys Acta,1977,487:297-306.
[36]Shu D P,Chen B L,Hong J,et al..Global transcriptional profiling in porcine mammary glands from late pregnancy to peak lactation.OMICS,2012,16:123-137.
[37]S(?)rensen M T,Sejrsen K,Purup S.Mammary gland development in gilts.Livest Produc Sci,2002,75:143-148.
[38]Stefanon B,Colitti M,Gabai G,et al..Mammary apoptosis and lactation persistency in dairy animals.J Dairy Res,2002,69:37-52.
[39]Theil P K,Labouriau R,Sejrsen K,et al..Expression of genes involved in regulation of cell turnover during milk stasis and lactation rescue in sow mammary glands.J Anim Sci,2005,83:2349-2356.
[40]Theil P K,Sejrsen K,Hurley W L,et al..Role of suckling in regulating cell turnover and onset and maintenance of lactation in individual mammary glands of sows.J Anim Sci,2006,84:1691-1698.
[41]Trottier N L,Shipley C F,Easter R A.Plasma amino acid uptake by the mammary gland of the lactating sow.J Anim Sci,1997,75:1266-1278.
[42]Winn R J,Baker M D,Merle C A,et al..Individual and combined effects of relaxin,estrogen,and progesterone in ovariectomized gilts.II.Effects on mammary development.Endocrinology,1994,135:1250-1255.