人参提取物对于α-MSH作用的小鼠黑色素瘤B16细胞黑色素生成作用机制研究
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- 英文论文题名：Ginseng Extract Inhibit α-Melanocyte-Stimulating Hormone-Induced Melanogenesis B16 Mouse Melanoma Cells
- 论文作者：姜锐 ; 刘建增 ; 徐晓浩 ; 王珂 ; 宋佳 ; 赵大庆 ; 孙立伟
- 英文论文作者：Rui Jiang ; Jian-Zeng Liu ; Xiao-Hao Xu ; Ke Wang ; Jia Song ; Da-Qing Zhao ; Li-Wei Sun ; Jilin Technology Innovation Center for Chinese Medicine Biotechnology ; College of Biology and Chemistry ; Beihua University ; Changchun University of Chinese Medicine
- 年：2016
- 作者机构：吉林省中药生物技术科技创新中心北华大学化学与生物学院;长春中医药大学研发中心;
- 论文关键词：人参 ; 黑色素生成 ; 氧化应激
- 英文论文关键词：Ginseng ; melanogenesis ; oxidative stress
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：TQ658
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：7
- 文件大小：842k
- 原文格式：O
- 会议级别：全国
摘要
目的:探讨人参提取物抑制细胞内黑色素生成作用机制。方法:采用碱裂解、酶促反应、免疫印迹、流式细胞术等方法检测人参提取物对于小鼠黑色素瘤B16细胞内黑色素生成以及氧化应激的影响。结果:人参提取物能够降低α-MSH刺激的B16细胞内黑色素含量,抑制酪氨酸酶活性及其蛋白表达;降低活性氧含量,提高SOD酶活性和GSH含量。上述作用与ROS抑制剂NAC相类似。结论:人参提取物可能是通过抑制氧化应激而抑制黑色素合成发挥美白作用。
Objective:The purpose of this study was to investigate the molecular mechanisms of ginseng extract on melanogenesis in B16 mouse melanoma cells.Methods:The effects on melanogenesis and oxidative stress of ginseng extract in a-melanocyte-stimulating hormone(α-MSH)-treated B16 cells were determined by alkaline lysis method,enzymatic reaction,western-blotting,and flow cytometry assay.Results:Ginseng extract significantly decreased melanin content,suppressed cellular tyrosinase activity,and reduced expression of tyrosinase protein to inhibit B16 cells melanogenesis induced by(α-MSH).They also depleted cellular reactive oxygen species(ROS) generation,increased superoxide dismutase(SOD) activity and glutathione(GSH) level effectively.The ROS scavenger N-acetyl-L-cysteine(NAC) had a similar inhibitory effect on melanogenesis.Conclusions:These results suggest that ginseng extract may inhibit melanogenesis by suppressing oxidative stress.
Objective:The purpose of this study was to investigate the molecular mechanisms of ginseng extract on melanogenesis in B16 mouse melanoma cells.Methods:The effects on melanogenesis and oxidative stress of ginseng extract in a-melanocyte-stimulating hormone(α-MSH)-treated B16 cells were determined by alkaline lysis method,enzymatic reaction,western-blotting,and flow cytometry assay.Results:Ginseng extract significantly decreased melanin content,suppressed cellular tyrosinase activity,and reduced expression of tyrosinase protein to inhibit B16 cells melanogenesis induced by(α-MSH).They also depleted cellular reactive oxygen species(ROS) generation,increased superoxide dismutase(SOD) activity and glutathione(GSH) level effectively.The ROS scavenger N-acetyl-L-cysteine(NAC) had a similar inhibitory effect on melanogenesis.Conclusions:These results suggest that ginseng extract may inhibit melanogenesis by suppressing oxidative stress.
引文
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[2]Jeong-Yeon Lim,Kyoko Ishiguro and Isao Kubo.Tyrosinase Inhibitory p-Coumaric Acid from Ginseng Leaves[J].Phytother.Res,1999,13,371-375.
[3]Lee,D Y;Cha,B J;Lee,Y S,etc.The potential of minor ginsenosides isolated from the leaves of Panax ginseng as inhibitors of melanogenesis.International journal of molecular sciences,2015,16(1),1677-1690.
[4]Christina Wasmeier,Alistair N.Hume,Giulia Bolasco,Miguel C.Seabra.Melanosomes at a glance.J Cell Sci,2008,121(24),3995-3999.
[5]Lin,J Y;Fisher,D E.Melanocyte biology and skin pigmentation[J].Nature,2007,445(7130),843-850.
[6]Meyskens,F L,Jr.;Farmer,P;Fruehauf,J P,Redox regulation in human melanocytes and melanoma.Pigment cell research/sponsored by the European Society for Pigment Cell Research and the International Pigment[J].Cell Society,2001,14(3),148-154.
[7]Yamamura,T;Onishi,J;Nishiyama,T,Antimelanogenic activity of hydrocoumarins in cultured normal human melanocytes by stimulating intracellular glutathione synthesis[J].Archives of dermatological research 2002,294,(8),349-354.