碳纳米纤维/碳纤维固相微萃取联用LC-MS/MS检测植物激素
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- 英文论文题名：CNFs/CFs solid phase microextraction combined LC-MS/MS for detection phytohormone
- 论文作者：邹依霖 ; 孟龙月 ; 王李原 ; 李东浩
- 英文论文作者：Yilin Zou ; Longyue Meng ; Liyuan Wang ; Donghao Li ; Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ; Ministry of Education (Yanbian University) ; Department of Chemistry ; Faculty of Science ; Yanbian University
- 年：2016
- 作者机构：延边大学长白山生物资源与功能分子教育部重点实验室;延边大学理学院化学系;
- 论文关键词：植物激素 ; 固相微萃取 ; 碳纳米纤维 ; LC-MS/MS
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：Q946.885;O658.2
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：258k
- 原文格式：D
- 会议级别：全国
摘要
植物激素调控植物生长周期的每个生理过程,植物激素检测已成为近十几年来研究热点,尤其是活体原位分析领域。固相微萃取技术集采样萃取分离浓缩为一体,为实时检测植物激素,提供新思路。但该技术应用于植物样品分析中,有萃取纤维头易受污染,涂层容易脱落,价格也比较昂贵,富集率低下等缺点。本研究采用化学气相沉积法制备碳纳米纤维/碳纤维固相微萃取材料,建立一种浸提微富集实时检测植物激素的色谱分析方法。通过将碳纳米纤维修饰在碳纤维上,形成空间立体三维结构,有效的增加活性位点,进而提高富集率,解决浓度低难以实现实时检测的难题。该方法能够快速准确分析植物激素(JA,IAA,ABA),整个过程4 min即可完成,实现了植物激素的快速分析。3种目标物的回收率为72%~87%,RSD为3.30%~7.52%(n=6)。该方法对探究各类激素间的相互关系、认识激素调控植物适应环境的机制以及遗传育种等方面具有重要的指导意义。
Phytohormones play a vital role in plant growth and metabolic processes.It is usually rather difficult to determine phytohormones in plant tissues owing to their presence in trace amounts and to the coexistence of a wide variety of interferens.An effective new technique was developed to help carbon nanofibers(CNFs) grow on carbon fibers.The new solid phase microextraction(SPME) device manufactured with CF-CNFs has been tested with three different phytohormones(IAA,JA,ABA) in aqueous solutions,showing a great extraction efficiency with short adsorption time(3 min).The recoveries of 3 targets are 72%~87%,RSD are 3.30%~7.52%(n = 6).This new sorbent material can be then considered a suitable candidate for on-site,in-site and in-vivo extraction of analytes from biological samples.
Phytohormones play a vital role in plant growth and metabolic processes.It is usually rather difficult to determine phytohormones in plant tissues owing to their presence in trace amounts and to the coexistence of a wide variety of interferens.An effective new technique was developed to help carbon nanofibers(CNFs) grow on carbon fibers.The new solid phase microextraction(SPME) device manufactured with CF-CNFs has been tested with three different phytohormones(IAA,JA,ABA) in aqueous solutions,showing a great extraction efficiency with short adsorption time(3 min).The recoveries of 3 targets are 72%~87%,RSD are 3.30%~7.52%(n = 6).This new sorbent material can be then considered a suitable candidate for on-site,in-site and in-vivo extraction of analytes from biological samples.
引文
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