Association between H3K4me3/BDNF and the cognitive function of workers occupationally exposed to Aluminum
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- 英文论文题名：Association between H3K4me3/BDNF and the cognitive function of workers occupationally exposed to Aluminum
- 论文作者：Qiu Hengying ; Ren Pei ; Li Rui ; Zhang Qinli ; Lu Xiaoting ; Niu Qiao
- 英文论文作者：Qiu Hengying ; Ren Pei ; Li Rui ; Zhang Qinli ; Lu Xiaoting ; Niu Qiao ; Department of Occupational and Environmental Health ; Shanxi Medical University
- 年：2016
- 作者机构：Department of Occupational and Environmental Health,Shanxi Medical University;
- 英文论文关键词：Aluminum ; cognitive function ; H3K4me3 ; BDNF
- 会议召开时间：2016-12-03
- 会议录名称：2016中国毒理学会神经毒理专业委员会学术年会论文集
- 语种：英文
- 分类号：R135
- 学会代码：ZGDV
- 会议名称：2016中国毒理学会神经毒理专业委员会学术年会
- 会议地点：中国北京
- 主办单位：中国毒理学会神经毒理专业委员会
- 学会名称：中国毒理学会
- 页数：1
- 文件大小：62k
- 原文格式：O
- 会议级别：全国
摘要
Objective Brain-derived neurotrophic factor(BDNF) involved in synaptic plasticity and may be modified by tri-methyl histone H3 lysine residues 4 points(H3K4me3),thus affecting learning and memory.To investigate the influence of occupational aluminum exposure on cognitive function and its relationship with H3K4me3 and BDNF levels.Methods By cluster random sampling method,a total of235 cases of male workers selected from a Shanxi Aluminum factory are recruited in the study in September 2014.Used the occupational epidemiological investigation questionnaire,which included Mini-Mental State Examination(MMSE),Clock Drawing Test(CDT),Digit Span Test(DST,including forward DSFT and backward test DSBT),Fuild Object Memory Evaluation(FOME) and Verbal Fluency Test(VFT),to collect subject' basic information and assess their cognitive function score.Detected the concentration of Aluminum in plasma by graphite furnace atomic absorption spectrometry.Subjects were divided into three groups by the 25 percentile and 75 percentile of the Aluminum content,such as low,middle and high Aluminum concentration group.The concentrations of H3K4me3 in lymphocyte and BDNF in plasma were determined by enzyme-linked immunosorbent assay.Results The levels of Aluminum in plasma was 134.36(100.14,178.96) μg/l.The scores of MMSE,DSFT,DSBT,DST of high Aluminum concentration group were lower than low Aluminum group(27.98+1.53 vs28.8311.54,9.19±2.00 vs 10.64+2.87,6.08±1.63 vs 7.19±3.07,15.27+3.11 vs 17.81±4.72,alLP<0.05),The scores of CDT,FOME,VFT among three groups had no statistical significance(all P>0.05).The expression levels of H3K4me3 and BDNF of high Aluminum concentration group were lower than the low group[(18.45±9.81) ng/ug protein vs(23.76±9.89) ng/ug protein,(26.07±10.18)ug/ml vs(31.66±9.24)ug/ml,all P <0.05].Multiple correlation analysis showed that Aluminum concentration were negatively correlated to H3K4me3,BDNF,MMSE,DSFT,DST,respectively(r_s=-0.307,-0.214,-0.252.-0.197,-0.181,all P<0.01).Conclusions Exposure to occupational Aluminum for a long time may change cognitive function,which go along with the decreasing of H3K4me3 level in lymphocyte and BDNF protein expression in plasma.
Objective Brain-derived neurotrophic factor(BDNF) involved in synaptic plasticity and may be modified by tri-methyl histone H3 lysine residues 4 points(H3K4me3),thus affecting learning and memory.To investigate the influence of occupational aluminum exposure on cognitive function and its relationship with H3K4me3 and BDNF levels.Methods By cluster random sampling method,a total of235 cases of male workers selected from a Shanxi Aluminum factory are recruited in the study in September 2014.Used the occupational epidemiological investigation questionnaire,which included Mini-Mental State Examination(MMSE),Clock Drawing Test(CDT),Digit Span Test(DST,including forward DSFT and backward test DSBT),Fuild Object Memory Evaluation(FOME) and Verbal Fluency Test(VFT),to collect subject' basic information and assess their cognitive function score.Detected the concentration of Aluminum in plasma by graphite furnace atomic absorption spectrometry.Subjects were divided into three groups by the 25 percentile and 75 percentile of the Aluminum content,such as low,middle and high Aluminum concentration group.The concentrations of H3K4me3 in lymphocyte and BDNF in plasma were determined by enzyme-linked immunosorbent assay.Results The levels of Aluminum in plasma was 134.36(100.14,178.96) μg/l.The scores of MMSE,DSFT,DSBT,DST of high Aluminum concentration group were lower than low Aluminum group(27.98+1.53 vs28.8311.54,9.19±2.00 vs 10.64+2.87,6.08±1.63 vs 7.19±3.07,15.27+3.11 vs 17.81±4.72,alLP<0.05),The scores of CDT,FOME,VFT among three groups had no statistical significance(all P>0.05).The expression levels of H3K4me3 and BDNF of high Aluminum concentration group were lower than the low group[(18.45±9.81) ng/ug protein vs(23.76±9.89) ng/ug protein,(26.07±10.18)ug/ml vs(31.66±9.24)ug/ml,all P <0.05].Multiple correlation analysis showed that Aluminum concentration were negatively correlated to H3K4me3,BDNF,MMSE,DSFT,DST,respectively(r_s=-0.307,-0.214,-0.252.-0.197,-0.181,all P<0.01).Conclusions Exposure to occupational Aluminum for a long time may change cognitive function,which go along with the decreasing of H3K4me3 level in lymphocyte and BDNF protein expression in plasma.
Objective Brain-derived neurotrophic factor(BDNF) involved in synaptic plasticity and may be modified by tri-methyl histone H3 lysine residues 4 points(H3K4me3),thus affecting learning and memory.To investigate the influence of occupational aluminum exposure on cognitive function and its relationship with H3K4me3 and BDNF levels.Methods By cluster random sampling method,a total of235 cases of male workers selected from a Shanxi Aluminum factory are recruited in the study in September 2014.Used the occupational epidemiological investigation questionnaire,which included Mini-Mental State Examination(MMSE),Clock Drawing Test(CDT),Digit Span Test(DST,including forward DSFT and backward test DSBT),Fuild Object Memory Evaluation(FOME) and Verbal Fluency Test(VFT),to collect subject' basic information and assess their cognitive function score.Detected the concentration of Aluminum in plasma by graphite furnace atomic absorption spectrometry.Subjects were divided into three groups by the 25 percentile and 75 percentile of the Aluminum content,such as low,middle and high Aluminum concentration group.The concentrations of H3K4me3 in lymphocyte and BDNF in plasma were determined by enzyme-linked immunosorbent assay.Results The levels of Aluminum in plasma was 134.36(100.14,178.96) μg/l.The scores of MMSE,DSFT,DSBT,DST of high Aluminum concentration group were lower than low Aluminum group(27.98+1.53 vs28.8311.54,9.19±2.00 vs 10.64+2.87,6.08±1.63 vs 7.19±3.07,15.27+3.11 vs 17.81±4.72,alLP<0.05),The scores of CDT,FOME,VFT among three groups had no statistical significance(all P>0.05).The expression levels of H3K4me3 and BDNF of high Aluminum concentration group were lower than the low group[(18.45±9.81) ng/ug protein vs(23.76±9.89) ng/ug protein,(26.07±10.18)ug/ml vs(31.66±9.24)ug/ml,all P <0.05].Multiple correlation analysis showed that Aluminum concentration were negatively correlated to H3K4me3,BDNF,MMSE,DSFT,DST,respectively(r_s=-0.307,-0.214,-0.252.-0.197,-0.181,all P<0.01).Conclusions Exposure to occupational Aluminum for a long time may change cognitive function,which go along with the decreasing of H3K4me3 level in lymphocyte and BDNF protein expression in plasma.
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