Phenylethanoid glycosides from Cistanche tubulosa inhibits the growth of B16-F10 cells both in vitro and in vivo by induction of apoptosis via mitochondrion-dependent pathway
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- 英文论文题名：Phenylethanoid glycosides from Cistanche tubulosa inhibits the growth of B16-F10 cells both in vitro and in vivo by induction of apoptosis via mitochondrion-dependent pathway
- 论文作者：Jinyu Li ; Jinyao Li ; Adila Aipire ; Li Gao ; Shixia Huo ; Jiaojiao Luo ; Fuchun Zhang
- 英文论文作者：Jinyu Li ; Jinyao Li ; Adila Aipire ; Li Gao ; Shixia Huo ; Jiaojiao Luo ; Fuchun Zhang ; Xinjiang Key Laboratory of Biological Resources and Genetic Engineering ; College of Life Science and Technology ; Xinjiang University ; Xinjiang Laboratory of Uighur Medical Prescription ; Xinjiang Institute of Traditional Uighur Medicine
- 年：2016
- 作者机构：Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;Xinjiang Laboratory of Uighur Medical Prescription,Xinjiang Institute of Traditional Uighur Medicine;
- 英文论文关键词：Phenylethanoid glycosides ; melanoma ; apoptosis ; caspases
- 会议召开时间：2016-09-01
- 会议录名称：全国农业生物化学与分子生物学第十五届学术研讨会会议文集
- 英文会议录名称：The Special Edition of the 15th National Symposium of Agricultural Biochemistry and Molecular Biology
- 语种：英文
- 分类号：R73-36
- 学会代码：IGSS
- 会议名称：全国农业生物化学与分子生物学第十五届学术研讨会
- 会议地点：中国甘肃兰州
- 主办单位：中国生物化学与分子生物学会农业分会
- 学会名称：中国生物化学与分子生物学会
- 页数：2
- 文件大小：650k
- 原文格式：O
- 会议级别：全国
摘要
Ethnopharmacological relevance:Cistanche has been used as traditional Chinese medicine for several thousands of years to treat various diseases including impotence,sterility and body weakness.Phenylethanoid glycosides from cistanche have been shown different biological activities including anti-allergy,anti-osteoporotic activity,hepatoprotective activity,glucose tolerance and bone regeneration.However,the anti-tumor activity of phenylethanoid glycosides from Cistanche needs to be investigated.Materials and methods:Cistanche tubulosa phenylethanoid glycosides(CTPG) were used to treat B16-F10 cells at different concentrations(0,100,200,300,and 400 μg/ml) in vitro for different times.The cell morphology,viability,cell cycle,apoptosis and necrosis were analysis by fluorescence microscope,MTT assay,Hoechst 33258 staining and flow cytometry,respectively.The expressions of pro- and anti-apoptotic proteins were detected by western blot.The in vivo anti-tumor effects were tested in tumor mouse model.Results:CTPG dramatically changed the morphology of B16-F10 cells,and significantly reduced the viability of B16-F10 cells in a dose-dependent and time-dependent manner.The results from Hoechst33258 staining and flow cytometry showed that CTPG treatment induced apoptosis and cell cycle arrest of B16-F10 cells.0-400 μg/ml of CTPG did not induce the apoptosis of 293 T cells.After CTPG treatment,the expressions of BAX and BCL-2 were up-regulated and down-regulated,respectively.Moreover,mitochondrial membrane potential was reduced and ROS generation was increased.Consequently,the levels of cytochrome c and cleaved-caspase-3 and-9 were up-regulated by CTPG treatment but not for cleaved-caspase-8.We further observed that subcutaneous injection of CTPG significantly inhibited the tumor growth in vivo and improved the survival rate of tumor mice.We also observed that CTPG promoted the proliferation of splenocytes and increased the proportions of CD4~+and CD8~+ T cells in spleens of tumor mice.
Ethnopharmacological relevance:Cistanche has been used as traditional Chinese medicine for several thousands of years to treat various diseases including impotence,sterility and body weakness.Phenylethanoid glycosides from cistanche have been shown different biological activities including anti-allergy,anti-osteoporotic activity,hepatoprotective activity,glucose tolerance and bone regeneration.However,the anti-tumor activity of phenylethanoid glycosides from Cistanche needs to be investigated.Materials and methods:Cistanche tubulosa phenylethanoid glycosides(CTPG) were used to treat B16-F10 cells at different concentrations(0,100,200,300,and 400 μg/ml) in vitro for different times.The cell morphology,viability,cell cycle,apoptosis and necrosis were analysis by fluorescence microscope,MTT assay,Hoechst 33258 staining and flow cytometry,respectively.The expressions of pro- and anti-apoptotic proteins were detected by western blot.The in vivo anti-tumor effects were tested in tumor mouse model.Results:CTPG dramatically changed the morphology of B16-F10 cells,and significantly reduced the viability of B16-F10 cells in a dose-dependent and time-dependent manner.The results from Hoechst33258 staining and flow cytometry showed that CTPG treatment induced apoptosis and cell cycle arrest of B16-F10 cells.0-400 μg/ml of CTPG did not induce the apoptosis of 293 T cells.After CTPG treatment,the expressions of BAX and BCL-2 were up-regulated and down-regulated,respectively.Moreover,mitochondrial membrane potential was reduced and ROS generation was increased.Consequently,the levels of cytochrome c and cleaved-caspase-3 and-9 were up-regulated by CTPG treatment but not for cleaved-caspase-8.We further observed that subcutaneous injection of CTPG significantly inhibited the tumor growth in vivo and improved the survival rate of tumor mice.We also observed that CTPG promoted the proliferation of splenocytes and increased the proportions of CD4~+and CD8~+ T cells in spleens of tumor mice.
Ethnopharmacological relevance:Cistanche has been used as traditional Chinese medicine for several thousands of years to treat various diseases including impotence,sterility and body weakness.Phenylethanoid glycosides from cistanche have been shown different biological activities including anti-allergy,anti-osteoporotic activity,hepatoprotective activity,glucose tolerance and bone regeneration.However,the anti-tumor activity of phenylethanoid glycosides from Cistanche needs to be investigated.Materials and methods:Cistanche tubulosa phenylethanoid glycosides(CTPG) were used to treat B16-F10 cells at different concentrations(0,100,200,300,and 400 μg/ml) in vitro for different times.The cell morphology,viability,cell cycle,apoptosis and necrosis were analysis by fluorescence microscope,MTT assay,Hoechst 33258 staining and flow cytometry,respectively.The expressions of pro- and anti-apoptotic proteins were detected by western blot.The in vivo anti-tumor effects were tested in tumor mouse model.Results:CTPG dramatically changed the morphology of B16-F10 cells,and significantly reduced the viability of B16-F10 cells in a dose-dependent and time-dependent manner.The results from Hoechst33258 staining and flow cytometry showed that CTPG treatment induced apoptosis and cell cycle arrest of B16-F10 cells.0-400 μg/ml of CTPG did not induce the apoptosis of 293 T cells.After CTPG treatment,the expressions of BAX and BCL-2 were up-regulated and down-regulated,respectively.Moreover,mitochondrial membrane potential was reduced and ROS generation was increased.Consequently,the levels of cytochrome c and cleaved-caspase-3 and-9 were up-regulated by CTPG treatment but not for cleaved-caspase-8.We further observed that subcutaneous injection of CTPG significantly inhibited the tumor growth in vivo and improved the survival rate of tumor mice.We also observed that CTPG promoted the proliferation of splenocytes and increased the proportions of CD4~+and CD8~+ T cells in spleens of tumor mice.
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