M protein of group A streptococcus plays an essential role in inducing high expression of A20 that in turn down-regulates inflammatory responses
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- 英文论文题名：M protein of group A streptococcus plays an essential role in inducing high expression of A20 that in turn down-regulates inflammatory responses
- 论文作者：Ma Cuiqing ; Gao Xue ; Zhang Ling ; Wang jiachao ; Han xiao ; Wei Lin
- 英文论文作者：Ma Cuiqing ; Gao Xue ; Zhang Ling ; Wang jiachao ; Han xiao ; Wei Lin ; Department of immunology ; Hebei Medical University
- 年：2016
- 作者机构：Department of immunology,Hebei Medical University;
- 英文论文关键词：Group A streptococcus(GAS) ; Macrophage ; proinflammatory cytokines ; A20
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R378
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1039k
- 原文格式：D
- 会议级别：全国
摘要
Objective: Group A streptococcus(GAS), a common pathogen, resides in host for a long time by evading host immune attack. One of the underlying mechanisms is to upregulate the expression of immunosuppressive molecules and therefore reduces inflammatory cytokine production in immune cells. Here, we aim to explore the the mechanisms induced by GAS.Methods and Results: The proinflammatory cytokines expressed by macrophages were down-regulated following GAS stimulation through ELISA and Real time PCR. Interestingly, we observed that knocking-down A20 with shRNAincreased the expression of proinflammatory cytokines in macrophages in response to GAS stimulation. When macrophages were stimulated by M protein-mutant GAS, A20 expression was significantly decreased compared with that by WT GAS Moreover, the purified M protein induced high expression of A20 in macrophages. Conclusions: M protein plays an essential role in inducing high A20 expression in macrophages, which in turn downregulates inflammatory cytokine response. As a consequence of proinflammatory cytokine inhibition, appropriate immune responses were dampened to allow GAS survival in host.
Objective: Group A streptococcus(GAS), a common pathogen, resides in host for a long time by evading host immune attack. One of the underlying mechanisms is to upregulate the expression of immunosuppressive molecules and therefore reduces inflammatory cytokine production in immune cells. Here, we aim to explore the the mechanisms induced by GAS.Methods and Results: The proinflammatory cytokines expressed by macrophages were down-regulated following GAS stimulation through ELISA and Real time PCR. Interestingly, we observed that knocking-down A20 with sh RNAincreased the expression of proinflammatory cytokines in macrophages in response to GAS stimulation. When macrophages were stimulated by M protein-mutant GAS, A20 expression was significantly decreased compared with that by WT GAS Moreover, the purified M protein induced high expression of A20 in macrophages. Conclusions: M protein plays an essential role in inducing high A20 expression in macrophages, which in turn downregulates inflammatory cytokine response. As a consequence of proinflammatory cytokine inhibition, appropriate immune responses were dampened to allow GAS survival in host.
Objective: Group A streptococcus(GAS), a common pathogen, resides in host for a long time by evading host immune attack. One of the underlying mechanisms is to upregulate the expression of immunosuppressive molecules and therefore reduces inflammatory cytokine production in immune cells. Here, we aim to explore the the mechanisms induced by GAS.Methods and Results: The proinflammatory cytokines expressed by macrophages were down-regulated following GAS stimulation through ELISA and Real time PCR. Interestingly, we observed that knocking-down A20 with sh RNAincreased the expression of proinflammatory cytokines in macrophages in response to GAS stimulation. When macrophages were stimulated by M protein-mutant GAS, A20 expression was significantly decreased compared with that by WT GAS Moreover, the purified M protein induced high expression of A20 in macrophages. Conclusions: M protein plays an essential role in inducing high A20 expression in macrophages, which in turn downregulates inflammatory cytokine response. As a consequence of proinflammatory cytokine inhibition, appropriate immune responses were dampened to allow GAS survival in host.
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