The study on Regulatory B cells in mice infected with Echinoccus granulosus
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- 英文论文题名：The study on Regulatory B cells in mice infected with Echinoccus granulosus
- 论文作者：Cao Wenyan
- 英文论文作者：Cao Wenyan ; State Key Laboratory Incubation Base of Xinjiang Major Diseases Research(2010DS890294) and Xinjiang Key Laboratory of Echinococcosis ; First Affiliated Hospital of Xinjiang Medical University ; College of Basic Medicine of Xinjiang Medical University
- 年：2016
- 作者机构：State Key Laboratory Incubation Base of Xinjiang Major Diseases Research(2010DS890294) and Xinjiang Key Laboratory of Echinococcosis,First Affiliated Hospital of Xinjiang Medical University;College of Basic Medicine of Xinjiang Medical University;
- 英文论文关键词：Echinococcus granulosus infection ; Regulatory B cells ; IL-10 ; TGF-β1
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：352k
- 原文格式：D
- 会议级别：全国
摘要
Objective:To observe the phenotype changes and expressions of regulatory B cells and its related molecules in BALB/c mice infected with Echinococcus granulosus and relationship between them and infection focus,in order to reaserch the relationship between regulatory B cells and its related molecules in Echinococcus granulosus infection and to reaserch the function of regulatory B cells to Echinococcus granulosus infection.Method:Flow cytometry was used to analyze the rate of CD1dhiCD5+CD19hi B cells and CD1dhiCD5+CD19hi IL-10+ B cells in spleen;The expressions of IL-10 and TGF-β1 m RNA were analyzed by q RT-PCR;The level of liver pathological alteration was observed by HE staining;The protein expression and location were analyzed by immuhistochemistry respectively;we measured the serum levels of IL-10 and TGF-β1 in each group by ELISA.Result:Flow cytometry results showed that the ratio of CD1dhiCD5+CD19hi cells and CD1dhiCD5+CD19hi IL-10+ cells in spleen infected mice was significantly higher from 90 days to 270 days after infection.q RT-PCR demonstrated that in the early stages,the expressions of IL-10 and TGF-β1 m RNA were increased in the middle and late stages of the infection.HE staining reveals that the erosion of livers was getting worse.IL-10 and TGF-β1 only observed in hepatic sinusoid,hyalomitome,fiber organization,focus germinal layer surrounding in infected group.5) CBA and ELISA results showed that the serum of IL-10 and TGF-β1 both remain low during early stages,but in the late stages,which significantly increased.Conclude:CD1dhiCD5+CD19hi regulatory B cells may be the one reason of leading Echinococcus granulosus infection immune suppress.Regulatory B cells in mice spleen participate in this development.In the infection,regulatory B cells by secreting IL-10 and TGF-β play inhibit function.Furthermore,the high expressions of regulatory B cells related molecules IL-10 and TGF-β1 are disadvantage of clearancing livers Echinococcus granulosus infection.
Objective:To observe the phenotype changes and expressions of regulatory B cells and its related molecules in BALB/c mice infected with Echinococcus granulosus and relationship between them and infection focus,in order to reaserch the relationship between regulatory B cells and its related molecules in Echinococcus granulosus infection and to reaserch the function of regulatory B cells to Echinococcus granulosus infection.Method:Flow cytometry was used to analyze the rate of CD1dhiCD5+CD19hi B cells and CD1dhiCD5+CD19hi IL-10+ B cells in spleen;The expressions of IL-10 and TGF-β1 m RNA were analyzed by q RT-PCR;The level of liver pathological alteration was observed by HE staining;The protein expression and location were analyzed by immuhistochemistry respectively;we measured the serum levels of IL-10 and TGF-β1 in each group by ELISA.Result:Flow cytometry results showed that the ratio of CD1dhiCD5+CD19hi cells and CD1dhiCD5+CD19hi IL-10+ cells in spleen infected mice was significantly higher from 90 days to 270 days after infection.q RT-PCR demonstrated that in the early stages,the expressions of IL-10 and TGF-β1 m RNA were increased in the middle and late stages of the infection.HE staining reveals that the erosion of livers was getting worse.IL-10 and TGF-β1 only observed in hepatic sinusoid,hyalomitome,fiber organization,focus germinal layer surrounding in infected group.5) CBA and ELISA results showed that the serum of IL-10 and TGF-β1 both remain low during early stages,but in the late stages,which significantly increased.Conclude:CD1dhiCD5+CD19hi regulatory B cells may be the one reason of leading Echinococcus granulosus infection immune suppress.Regulatory B cells in mice spleen participate in this development.In the infection,regulatory B cells by secreting IL-10 and TGF-β play inhibit function.Furthermore,the high expressions of regulatory B cells related molecules IL-10 and TGF-β1 are disadvantage of clearancing livers Echinococcus granulosus infection.
Objective:To observe the phenotype changes and expressions of regulatory B cells and its related molecules in BALB/c mice infected with Echinococcus granulosus and relationship between them and infection focus,in order to reaserch the relationship between regulatory B cells and its related molecules in Echinococcus granulosus infection and to reaserch the function of regulatory B cells to Echinococcus granulosus infection.Method:Flow cytometry was used to analyze the rate of CD1dhiCD5+CD19hi B cells and CD1dhiCD5+CD19hi IL-10+ B cells in spleen;The expressions of IL-10 and TGF-β1 m RNA were analyzed by q RT-PCR;The level of liver pathological alteration was observed by HE staining;The protein expression and location were analyzed by immuhistochemistry respectively;we measured the serum levels of IL-10 and TGF-β1 in each group by ELISA.Result:Flow cytometry results showed that the ratio of CD1dhiCD5+CD19hi cells and CD1dhiCD5+CD19hi IL-10+ cells in spleen infected mice was significantly higher from 90 days to 270 days after infection.q RT-PCR demonstrated that in the early stages,the expressions of IL-10 and TGF-β1 m RNA were increased in the middle and late stages of the infection.HE staining reveals that the erosion of livers was getting worse.IL-10 and TGF-β1 only observed in hepatic sinusoid,hyalomitome,fiber organization,focus germinal layer surrounding in infected group.5) CBA and ELISA results showed that the serum of IL-10 and TGF-β1 both remain low during early stages,but in the late stages,which significantly increased.Conclude:CD1dhiCD5+CD19hi regulatory B cells may be the one reason of leading Echinococcus granulosus infection immune suppress.Regulatory B cells in mice spleen participate in this development.In the infection,regulatory B cells by secreting IL-10 and TGF-β play inhibit function.Furthermore,the high expressions of regulatory B cells related molecules IL-10 and TGF-β1 are disadvantage of clearancing livers Echinococcus granulosus infection.
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