基于植物代谢组学的栽培型与野生型野菊花的化学成分比较及定量分析
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- 英文论文题名：Chemical comparison and determination of cultivated and wild type Flos Chrysanthemi Indici by plant metabolomics approach and UPLC
- 论文作者：杨勇 ; 韩正洲 ; 贾红梅 ; 魏民 ; 马庆 ; 詹若挺 ; 陈蔚文 ; 邹忠梅
- 英文论文作者：YANG Yong ; HAN Zheng-zhou ; JIA Hong-mei ; WEI Ming ; MA Qing ; ZHAN Ruo-ting ; CHEN Wei-wen ; ZOU Zhong-mei ; Institute of Medicinal Plant Development ; Chinese Academy of Medical Sciences and Peking Union Medical College ; Research Center of Chinese Herbal Resource Science and Engineering ; Guangzhou University of Traditional Chinese Medicine ; Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Chinese Medicine) ; Ministry of Education ; China Resources Sanjiu Medical & Pharmaceutical Co. ; Ltd.
- 年：2016
- 作者机构：中国医学科学院北京协和医学院药用植物研究所;广州中医药大学中药资源科学与工程研究中心岭南中药资源教育部重点实验室(广州中医药大学);华润三九医药股份有限公司;
- 论文关键词：野菊花 ; 栽培型 ; 野生型 ; 植物代谢组学 ; 化学成分
- 英文论文关键词：Flos Chrysanthemi Indici ; cultivated type ; wild type ; plant metabolomics ; ingredients
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R284
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：8
- 文件大小：1099k
- 原文格式：O
- 会议级别：全国
摘要
目的为研究栽培型与野生型野菊花药材之间的化学成分差异。方法采用基于超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-T0F/MS)技术结合化学计量学对栽培和野生型野菊花药材的整体化学轮廓进行比较,并采用超高效液相(UPLC)对其中的主要差异成分进行定量分析。结果主成分分析(PCA)分析结果显示栽培型与野生型野菊花在主成分t[1]方向有明显区分,表明两者的化学组成存在显著差异。随后采用正交偏最小二乘法判别分析(0PLS-DA)方法筛选和鉴定两者的差异成分,结果显示,香叶木素-7-0-芸香苷、刺槐素-7-0-(6″-0-α-L-鼠李吡喃糖-β-槐糖苷)、木犀草素、蒙花苷、5-羟基-4′-甲氧基-黄酮-7-0-α-L-吡喃鼠李糖(1→6)[2-0-乙酰-β-D-吡喃葡萄糖(1→2)]-β-D吡喃葡萄糖苷、芹菜素、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸和刺槐素含量在两者间存在显著性差异,可以作为区分栽培和野生野菊花的特征性成分。结论植物代谢组学技术能快速筛选特征性化学成分,为野菊花的质量评价提供有效的手段。
OBJECTIVE To evaluate the quality of cultivated and wild Flos Chrysanthemi Indici and to uncover the differential chemical ingredients and the contents.METHODS An integrated strategy of global chemical profiling using ultra performance liquid chromatography coupled with tandem quadmpole time-of-flight mass spectrometry and chemometric approach was applied to compare the global chemical profiling of cultivated and wild Flos Chrysanthemi Indici,then determination the main differential ingredients by UPLC.RESULTS Principal component analysis showed that wild and cultivated Flos Chrysanthemi Indici could be separated clearly,suggesting a big chemical difference existed between them.Supervised OPLS-DA analysis was applied to screening and identifying the differential ingredients.As a result,nine ingredients including diosmetin-7-O-rutinoside,acacetin-7-O-(6"-O-α-L-rhamnopyranosyl-β-sophoroside),luteolin,linarin,5-hydroxy-4'-methoxy-flavone-7-O-α-L-rhamnopyranosyl(1→6)[2-O-acetyl-β-D-glucopyr anosyl(1→2)]-β-Dglucopyranoside,apigenin,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid and acacetin have a significant difference between cultivated and wild types,which could been considered as characteristic chemical markers for distinguishing cultivated Flos Chrysanthemi Indici from wild type.CONCLUSION This metabolomics-based approach is effective to screening characteristic chemical markers for quality evaluation of Flos Chrysanthemi Indici.
OBJECTIVE To evaluate the quality of cultivated and wild Flos Chrysanthemi Indici and to uncover the differential chemical ingredients and the contents.METHODS An integrated strategy of global chemical profiling using ultra performance liquid chromatography coupled with tandem quadmpole time-of-flight mass spectrometry and chemometric approach was applied to compare the global chemical profiling of cultivated and wild Flos Chrysanthemi Indici,then determination the main differential ingredients by UPLC.RESULTS Principal component analysis showed that wild and cultivated Flos Chrysanthemi Indici could be separated clearly,suggesting a big chemical difference existed between them.Supervised OPLS-DA analysis was applied to screening and identifying the differential ingredients.As a result,nine ingredients including diosmetin-7-O-rutinoside,acacetin-7-O-(6"-O-α-L-rhamnopyranosyl-β-sophoroside),luteolin,linarin,5-hydroxy-4'-methoxy-flavone-7-O-α-L-rhamnopyranosyl(1→6)[2-O-acetyl-β-D-glucopyr anosyl(1→2)]-β-Dglucopyranoside,apigenin,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid and acacetin have a significant difference between cultivated and wild types,which could been considered as characteristic chemical markers for distinguishing cultivated Flos Chrysanthemi Indici from wild type.CONCLUSION This metabolomics-based approach is effective to screening characteristic chemical markers for quality evaluation of Flos Chrysanthemi Indici.
引文
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[2]WU XS,XU J,CHEN CQ,et al.Research progress in chemical constituents of Chrysanthemum indicum and their quality assessment[J].Chin Tradit Herb Drugs(中草药),2015,46(3):443-452.
[3]DAN M,GAO XF,JIA W,et al.Application of metabolomics in research of plant metabolites[J].China J Chin Mater Med(中国中药杂志),2007,32(22):2337-2341.
[4]Mao Q,Bai M,Li SL,et al.Discrimination of leaves of Panax ginseng and P.quinquefolius by ultra high performance liquid chromatography quadrupole/time-of-flight mass spectrometry based metabolomics approach[J].JPharm Biomed Anal,2014,97:129-140.
[5]Yun DY,Kang YG,Hong YS,et al.Distinctive metabolism of flavonoid between cultivated and semiwild soybean unveiled through metabolomics approach[J].JAgr Food Chem.2016,64(29):5773-5783.
[6]Zhang HM,Li SL,Xu HX,et al.Holistic quality evaluation of commercial white and red ginseng using a UPLC-QTOF-MS/MS-based metabolomics approach[J].JPharm Biomed Anal,2012,62:258-273.
[7]Lv MY,Sun JB,Zhang ZJ,et al.GC-MS based metabolomics study of stems and roots of Ephedra sinica[J].J Pharm Biomed Anal,2015,114:49-52.
[8]Xie GX,Plumb R,Jia W,et al.Ultra-performance LC/TOF MS analysis of medicinal Panax herbs for metabolomic research[J].J Sep Sci,2008,31:1015-1026.
[9]ZHANG ZZ,ZHI HJ,LI ZY,et al.Chemical comparison of different Farfarae Flos by NMR-based metabolomic approaches[J].Acta Pharm Sin(药学学报),2015,50(5):599-604.
[10]Shariff MI,Gomaa AI,Cox IJ,et al.Urinary metabolic biomarkers of hepatocellular carcinoma in an Egyptian population:a validation study[J].J Proteome Res,2011,10(4):1828-1836.
[11]Wu QZ,Zhao DX,Xiang J,et al.Antitussive,expectorant,and anti-inflammatory activities of four caffepylquinic acids isolated from Tussilago farfara[J].Pharm Biol,2016,54(7):1117-1124.
[12]Chen X,Miao JS,Yan M,et al.The anti-inflammatory activities of Ainsliaea fragrans Champ,extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-kB pathway[J].J Ethnopharmacol,2015,170:72-80.
[13]Zhong RF,Xu GB,Wang Z,et al.Identification of anti-inflammatory constituents from Kalimeris indica with UHPLC-ESI-Q-TOF-MS/MS and GC-MS[J].JEthnopharmacol,2015,13:39-45.
[14]Huang YT,Hwang JJ,Lee PP,et al.Effects of luteolin and quercetin,inhibitors of tyrosine kinase,on cell growth and metastasis-associated properties in A431 cells overexpressing epidermal growth factor receptor[J].Brit J Pharmacol,1999,128:999-1010.
[15]SUN B,JU WJ,ZHANG XL.Advance in studies on pharmacological effects of apigenin[J].J Chin Med Mater(中药材),2004,27(7):531-534.
[16]ZHOU YY,ZHONG BH,SHI WG.Advance in studies on bioactivity of acacetin[J].Chin J New Drugs(中国新药杂志),2014,23(9):1053-1056.
[17]WU MX,WANG JJ,ZHANG GJ.Determination of the seven active components in water extraction of Chrysanthemi Indici Flos[J].Chin Tradit Pat Med(中成药),2011,33(2):300-304.
[18]Zhang QL,Li J,Wang C,et al.A gradient HPLC method for the quality control of chlorogenic acid,linarin and luteolin in Flos Chrysanthemi Indici suppository[J].J Pharm Biomed Anal,2007,43:753-757.
[19]WU XS,WANG J,XU J,et al.Determination for contents of linarin and chlorogenic acid in different parts of Chrysanthemum indicum by HPLC-DAD.Chin Tradit Herb Drugs(中草药),2014,45(11):1634-1636.
[20]HAN YC,GONG HY,LIU W,et al.Simultaneous determination of chlorogenic acid and buddlepside in Chrysanthemi Indici Flos by UPLC[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19(20):88-90.
[21]HE XZ,GUO Y,YU CY,et al.Comparison analysis of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in different parts of Chrysanthemum indicum[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19(20):88-90.