DNA折纸术在实现光热-化疗协同治疗的研究
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- 英文论文题名：DNA Origami as Carrier to Integrate Chemotherapy and Photothermal Therapy
- 论文作者：宋琳琳 ; 蒋乔 ; 丁宝全
- 英文论文作者：Linlin Song ; Qiao Jiang ; Baoquan Ding ; National Center for Nanoscience and Technology
- 年：2016
- 作者机构：国家纳米科学中心;
- 论文关键词：DNA折纸术 ; 阿霉素 ; 金纳米棒 ; 化学治疗 ; 光热治疗
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：R73-36;TB383.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：2
- 文件大小：211k
- 原文格式：D
- 会议级别：全国
摘要
DNA折纸术具有良好的可寻址性、可编程性和生物相容性,因此,DNA折纸结构被广泛用于纳米组装1和生物医药领域2-3。另一方面,贵金属纳米颗粒具有很强的局域表面等离子共振效应,可以实现光热治疗作用,在众多贵金属纳米颗粒中,金纳米棒具备容易合成、尺寸可控、便于修饰等优点,因而被广泛研究。实验表明,DNA折纸结构可以特异性组装化学药物或者金属纳米颗粒,用于抑制肿瘤的增长。基于以上研究,我们利用DNA折纸结构同时运载了金纳米棒和阿霉素,用来实现逆转耐药肿瘤细胞的耐药性的作用。其中阿霉素可以嵌入DNA双螺旋结构,经寡核苷酸序列修饰的金纳米棒可以特异性组装到折纸结构上。在近红外光的作用下,金纳米棒释放热量,有效地破坏了细胞表面的P糖蛋白结构,同时促进阿霉素从DNA折纸结构中释放出来。利用噻唑蓝显色实验分析,在光热治疗和化疗的共同作用下,细胞活力下降至20%。通过这个工作,我们实现了基于DNA纳米自组装实现光热-化疗对癌细胞的协同增效作用。
To develop smart nanotheranostic system, we manipulate DNA origami as the template to integrate with multiple functional elements into one platform. Organization of multiple photothermal conversion gold nanorods at pre-designed locations of origami template is achieved. Tumor-targeting apatmers and DNA intercalated drugs(doxorubicin) are also assembled into the same template. Our study here will not only offer new insights into the understanding of multifunctional nanotheranostics but also encourage the development of DNA platform as efficient and biocompatible candidates for novel antitumor drug delivery systems.
To develop smart nanotheranostic system, we manipulate DNA origami as the template to integrate with multiple functional elements into one platform. Organization of multiple photothermal conversion gold nanorods at pre-designed locations of origami template is achieved. Tumor-targeting apatmers and DNA intercalated drugs(doxorubicin) are also assembled into the same template. Our study here will not only offer new insights into the understanding of multifunctional nanotheranostics but also encourage the development of DNA platform as efficient and biocompatible candidates for novel antitumor drug delivery systems.
引文
[1]Qian Zhang,Qiao Jiang,Na Li,Luru Dai,Qing Liu,Linlin Song,Jinye Wang,Yaqian Li,Jie Tian,Baoquan Ding*,and Yang Du*,ACS nano.2014,8:6633–6643.
[2]Qiao Jiang,Yuefeng Shi,Qian Zhang,Na Li,Pengfei Zhan,Linlin Song,Luru Dai,Jie Tian,Yang Du*,Zhen Cheng*,and Baoquan Ding*,Small,2015,11:5134–5141.
[3]Qiao Jiang,Chen Song,Jeanette Nangreave,Xiaowei Liu,Lin Lin,Dengli Qiu,Zhen-Gang Wang,Guozhang Zou,Xingjie Liang,Hao Yan,*and Baoquan Ding*,J.Am.Chem.Soc.2012,134:13396-13403.
[2]Qiao Jiang,Yuefeng Shi,Qian Zhang,Na Li,Pengfei Zhan,Linlin Song,Luru Dai,Jie Tian,Yang Du*,Zhen Cheng*,and Baoquan Ding*,Small,2015,11:5134–5141.
[3]Qiao Jiang,Chen Song,Jeanette Nangreave,Xiaowei Liu,Lin Lin,Dengli Qiu,Zhen-Gang Wang,Guozhang Zou,Xingjie Liang,Hao Yan,*and Baoquan Ding*,J.Am.Chem.Soc.2012,134:13396-13403.