Isolation and Identification of Antagonistic Bacteria Against Soil-borne Pathogenic Fungus of Tobacco
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- 英文论文题名：Isolation and Identification of Antagonistic Bacteria Against Soil-borne Pathogenic Fungus of Tobacco
- 论文作者：宋喜乐 ; DING Yue-qi ; 康业斌
- 英文论文作者：SONG Xi-le ; DING Yue-qi ; KANG Ye-bin ; College of Forestry ; Henan University of Science and Technology
- 年：2016
- 作者机构：College of Forestry,Henan University of Science and Technology;
- 英文论文关键词：Tobacco ; Soil-born pathogenic fungi ; Antagonistic bacteria ; Paenibacillus polymyxa
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.72;S476.1
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：40k
- 原文格式：O
- 会议级别：全国
摘要
This study is aimed to isolate and screen bacteria strains from tobacco rhizospheric soil,which have antagonistic activities against soil-borne pathogenic fungus of tobacco.Soil dilution plating technique and dual culture technique on agar plate were used for isolate and screen antagonistic bacteria.The antagonistic strains were identified by morphological features observation,physiological and biochemical experiments,and 16 S rDNA sequence analysis.30 rhizospheric soil were collected from tobacco filed in Luoning,Ruyang,Songxian and Yiyang countries in Luoyang,Henan province.180 bacterial isolates were obtained from 30 rhizosphere soil samples by separation and purification,and 49 strains have antagonistic function to the soil-born pathogenic fungi tested in this trial.Strains TS681、TS682、TS683were strongly antagonistic to three kinds of pathogenic fungi,and the inhibition zones to Pythium aphanidermatum,Phytophthora parasitica and Furasium oxysporum respectively more than 7.0 mm,10.0 mm and 9.0mm with the antagonistic rate respectively reaching above 51.4%,68.8%and 60.0%.All this three strains were identified as Paenibacillus polymyxa,and they may have the potential value of development and application for biological controlling of soil-borne fungi diseases of tobacco.
This study is aimed to isolate and screen bacteria strains from tobacco rhizospheric soil,which have antagonistic activities against soil-borne pathogenic fungus of tobacco.Soil dilution plating technique and dual culture technique on agar plate were used for isolate and screen antagonistic bacteria.The antagonistic strains were identified by morphological features observation,physiological and biochemical experiments,and 16 S rDNA sequence analysis.30 rhizospheric soil were collected from tobacco filed in Luoning,Ruyang,Songxian and Yiyang countries in Luoyang,Henan province.180 bacterial isolates were obtained from 30 rhizosphere soil samples by separation and purification,and 49 strains have antagonistic function to the soil-born pathogenic fungi tested in this trial.Strains TS681、TS682、TS683were strongly antagonistic to three kinds of pathogenic fungi,and the inhibition zones to Pythium aphanidermatum,Phytophthora parasitica and Furasium oxysporum respectively more than 7.0 mm,10.0 mm and 9.0mm with the antagonistic rate respectively reaching above 51.4%,68.8%and 60.0%.All this three strains were identified as Paenibacillus polymyxa,and they may have the potential value of development and application for biological controlling of soil-borne fungi diseases of tobacco.
This study is aimed to isolate and screen bacteria strains from tobacco rhizospheric soil,which have antagonistic activities against soil-borne pathogenic fungus of tobacco.Soil dilution plating technique and dual culture technique on agar plate were used for isolate and screen antagonistic bacteria.The antagonistic strains were identified by morphological features observation,physiological and biochemical experiments,and 16 S rDNA sequence analysis.30 rhizospheric soil were collected from tobacco filed in Luoning,Ruyang,Songxian and Yiyang countries in Luoyang,Henan province.180 bacterial isolates were obtained from 30 rhizosphere soil samples by separation and purification,and 49 strains have antagonistic function to the soil-born pathogenic fungi tested in this trial.Strains TS681、TS682、TS683were strongly antagonistic to three kinds of pathogenic fungi,and the inhibition zones to Pythium aphanidermatum,Phytophthora parasitica and Furasium oxysporum respectively more than 7.0 mm,10.0 mm and 9.0mm with the antagonistic rate respectively reaching above 51.4%,68.8%and 60.0%.All this three strains were identified as Paenibacillus polymyxa,and they may have the potential value of development and application for biological controlling of soil-borne fungi diseases of tobacco.
引文