产絮菌A9的等离子体诱变及其特性研究
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摘要
采用等离子体诱变系统对微生物絮凝剂产生菌株类芽孢杆菌A9进行诱变选育,在电源功率为300W,工作气流为12L/min,等离子体发射源与样品之间的高度为5mm,氮气作为工作气体,照射时间为60s的条件下,筛选出一株高效产微生物絮凝剂的突变菌株A9-60,传代实验验证,该突变菌株遗传性能稳定。摇瓶实验发现:与出发菌株A9相比,突变菌株A9-60多糖产量提高22.3%,絮凝率提高4.83%。利用Boltzmann模型和DoseResp模型对诱变前后菌体生长和基质消耗动力学特征进行分析,发现突变菌株A9-60的生长周期较出发菌株A9缩短约6.8h,对发酵液中葡萄糖利用能力增加13.22%,结果表明,等离子体诱变技术是获得高效产微生物絮凝剂的产絮菌的有效途径之一。
引文
[1]SUN Pengxuan(孙鹏轩).Research Progress and Application Status of bioflocculant[J].Environmental Protection and Recycling Economy(环境保护与循环经济),2013,(1):53-55
[2]Guo Junyuan,Zhang Yuzhe,Zhao Jing,et aL Characterization of a Bioflocculant from Potato Starch Waste Water and its Application in Sludge Dewatering[J].Biotechnological Products And Process Engineering,2015,99:5429-5437.
[3]YAO Li(姚力),XIN Xin(姚力),YANG Yijin(羊依金),et al.Treatment of Printing and Dyeing Wastewater by a New Type of Composite Bioflocculant[J].Industrial Water Treatment(工业水处理),2014,34(8):65-68
[4]CUI Li(崔丽),WANG Ye(王业),HU Xiao-min(胡筱敏),et a1.An experimental research on culture medium optimization of a flocculant-producing strain[J].Journal of Safety and Environment(安全与环境学报),2007,3(7):10-12.
[5]Simphiwe P.B,Ademola O.O,Balakrishna P.Production and Characterization of Bioflocculants from Bacteria Isolated from Wastewater Treatment Plant in South Africa[J].Biotechnology and Bioprocess Engineering,2010,15:874-881.
[6]Liang MingHua,LIANG XiaoNa.Mutation Breeding of Saccharomyces cerevisiae with Lower Methanol Content and the Effects of Pectinase,Cellulase and Glycine in Sugarcane Spirits[J].Society of Chemical Industry,2015,95:1949-1955
[7]Wang Chao,ZHANG Linlin,XU Hong.The Effects of N*Ion Implantation Mutagenesis on the LaccaseProduction of Ceriporiopsis subvermispora[J].Biotechnology and Bioprocess Engineering,2012,17;946-951
[8]Siddique AwanM,Nabila T,Ayub N,et aL Gamma Radiation Induced Mutagenesis in Aspergillusniger to Enhance its Microbial Fermentation Activity for Industrial enzyme Production[J].Molecular Biology Reports,2011,38:1367-1374
[9]Jung IP,Cho JH,KooBS,et al.Functional evaluation of residues in the herbicide-binding site of Mycobacterium tuberculosisacelo hydroxyacid synthase by Site-directed Mutagenesis[J].Enzyme and Microbial Technology,2015,78:18-26
[10]LI Peirui(李培睿),YAN Shiliang(闫世梁),ZHANG Donghui(张东辉),et al.Study On Mutagenic Effects Bioflocculant Producing Strains By Nitrogen Ion Implantation[J].Nuclear Techniques(核技术),2008,31(3):193-196
[11]Yang YN,RenN,XueJM,et al.Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus[J].Nuclear Instruments and Methods in Physics Research B,2007,262:220-224
[12]ZHAO Xiaoxiang(赵晓祥),ZHAN Xiaojing(詹小菁).the Research on Batch Cultivation and Mutagenesis for Bioflocculant[J].Environmental Pollution and Control(环境污染与防治),2007,29(12):900-904
[13]TIAN Liansheng(田连生),CHEN Xiuqing(陈秀清).Mutation Breeding of High Efficiency Bioflocculan tProducing Strain[J].Industrial Safety and Environmental Protection(工业安全与环保),2015,41(7):43-45
[14]LI Xia(李侠),PENG Dan(彭丹),ZHANG Chunhui(张春晖),et al.Fermentation Dynamics of Red Data Wine[J].China Brewing(中国酿造),2012,31(1):190-193
[15]SUN Xiang(孙祥),LOU Chong(楼崇),JIANG Jinfan(江津凡).Study on the Shooting and Height Growth of P.vivaxf.huan yen zhnJ.L Lu[J].Journal of Fujian Forestry Sci and Technology(福建林业科技),2010,37(4):98-101
[16]WANG Kaixuan(王凯旋).Optimization of Mulberry Processing Technology and Development of a New-Type Mulberry Fruit TabletBased on Conversion by Lactic Acid Bacteria[D](.基于乳酸菌转化的桑椹加工工艺优化及桑椹果片的研制).Zhen Jiang:Jiangsu University of Science and Technology,2013
[17]ZHANG Huaiqiang(张怀强).Comparison of Growth Kinetics and Physiologieal Characteristics of E eoli CVCC249 under both Bateh and Continuous Culture[D](大肠杆菌E coli CVCC249在分批和连续培养条件下的生长动力学和生理特性比较研究).Ji Nan:Shandong University,2008
[18]WANG xue(王雪).Optimum Fermentation Condition and Kinetics for Bioflocculant by Mixed Strains[D](生物絮凝剂混菌发酵条件优化及动力学分析).ha er bin:Harbin Institute of Technology
[19]Jiang Binhui,Zhao Xin,LIU Jinliang,et al.Paenibacillus Shenyangensis Sp.Nov.,a:Bioflocculant-Producing Species Isolated from Soil Under a Peach Tree[J].International Journal of Systematic and Evolutionary Microbiology,2015,65:220-224
[20]DONG Linqing(董林青),NIU Guowen(牛国文),LIU Zhiyi(刘志毅),et al.Determination of the Sugar Content in Fermentation Broth via Applications of Different Methods[J].Guangdong chemical industry(广东化工),2009,36(9):177-189
[21]QIU Zhongping(邱忠平),MAO Canquan(茆灿泉),LIU Yuanyue(刘源月),et al.Screening of a Bioflocculant Producing Strain and its Flocculation Characteristics[J].Chinese Journal of Environmental Engineering(环境工程学报),2009,3(7):1185-1188
[2]Guo Junyuan,Zhang Yuzhe,Zhao Jing,et aL Characterization of a Bioflocculant from Potato Starch Waste Water and its Application in Sludge Dewatering[J].Biotechnological Products And Process Engineering,2015,99:5429-5437.
[3]YAO Li(姚力),XIN Xin(姚力),YANG Yijin(羊依金),et al.Treatment of Printing and Dyeing Wastewater by a New Type of Composite Bioflocculant[J].Industrial Water Treatment(工业水处理),2014,34(8):65-68
[4]CUI Li(崔丽),WANG Ye(王业),HU Xiao-min(胡筱敏),et a1.An experimental research on culture medium optimization of a flocculant-producing strain[J].Journal of Safety and Environment(安全与环境学报),2007,3(7):10-12.
[5]Simphiwe P.B,Ademola O.O,Balakrishna P.Production and Characterization of Bioflocculants from Bacteria Isolated from Wastewater Treatment Plant in South Africa[J].Biotechnology and Bioprocess Engineering,2010,15:874-881.
[6]Liang MingHua,LIANG XiaoNa.Mutation Breeding of Saccharomyces cerevisiae with Lower Methanol Content and the Effects of Pectinase,Cellulase and Glycine in Sugarcane Spirits[J].Society of Chemical Industry,2015,95:1949-1955
[7]Wang Chao,ZHANG Linlin,XU Hong.The Effects of N*Ion Implantation Mutagenesis on the LaccaseProduction of Ceriporiopsis subvermispora[J].Biotechnology and Bioprocess Engineering,2012,17;946-951
[8]Siddique AwanM,Nabila T,Ayub N,et aL Gamma Radiation Induced Mutagenesis in Aspergillusniger to Enhance its Microbial Fermentation Activity for Industrial enzyme Production[J].Molecular Biology Reports,2011,38:1367-1374
[9]Jung IP,Cho JH,KooBS,et al.Functional evaluation of residues in the herbicide-binding site of Mycobacterium tuberculosisacelo hydroxyacid synthase by Site-directed Mutagenesis[J].Enzyme and Microbial Technology,2015,78:18-26
[10]LI Peirui(李培睿),YAN Shiliang(闫世梁),ZHANG Donghui(张东辉),et al.Study On Mutagenic Effects Bioflocculant Producing Strains By Nitrogen Ion Implantation[J].Nuclear Techniques(核技术),2008,31(3):193-196
[11]Yang YN,RenN,XueJM,et al.Mutation effect of MeV protons on bioflocculant bacteria Bacillus cereus[J].Nuclear Instruments and Methods in Physics Research B,2007,262:220-224
[12]ZHAO Xiaoxiang(赵晓祥),ZHAN Xiaojing(詹小菁).the Research on Batch Cultivation and Mutagenesis for Bioflocculant[J].Environmental Pollution and Control(环境污染与防治),2007,29(12):900-904
[13]TIAN Liansheng(田连生),CHEN Xiuqing(陈秀清).Mutation Breeding of High Efficiency Bioflocculan tProducing Strain[J].Industrial Safety and Environmental Protection(工业安全与环保),2015,41(7):43-45
[14]LI Xia(李侠),PENG Dan(彭丹),ZHANG Chunhui(张春晖),et al.Fermentation Dynamics of Red Data Wine[J].China Brewing(中国酿造),2012,31(1):190-193
[15]SUN Xiang(孙祥),LOU Chong(楼崇),JIANG Jinfan(江津凡).Study on the Shooting and Height Growth of P.vivaxf.huan yen zhnJ.L Lu[J].Journal of Fujian Forestry Sci and Technology(福建林业科技),2010,37(4):98-101
[16]WANG Kaixuan(王凯旋).Optimization of Mulberry Processing Technology and Development of a New-Type Mulberry Fruit TabletBased on Conversion by Lactic Acid Bacteria[D](.基于乳酸菌转化的桑椹加工工艺优化及桑椹果片的研制).Zhen Jiang:Jiangsu University of Science and Technology,2013
[17]ZHANG Huaiqiang(张怀强).Comparison of Growth Kinetics and Physiologieal Characteristics of E eoli CVCC249 under both Bateh and Continuous Culture[D](大肠杆菌E coli CVCC249在分批和连续培养条件下的生长动力学和生理特性比较研究).Ji Nan:Shandong University,2008
[18]WANG xue(王雪).Optimum Fermentation Condition and Kinetics for Bioflocculant by Mixed Strains[D](生物絮凝剂混菌发酵条件优化及动力学分析).ha er bin:Harbin Institute of Technology
[19]Jiang Binhui,Zhao Xin,LIU Jinliang,et al.Paenibacillus Shenyangensis Sp.Nov.,a:Bioflocculant-Producing Species Isolated from Soil Under a Peach Tree[J].International Journal of Systematic and Evolutionary Microbiology,2015,65:220-224
[20]DONG Linqing(董林青),NIU Guowen(牛国文),LIU Zhiyi(刘志毅),et al.Determination of the Sugar Content in Fermentation Broth via Applications of Different Methods[J].Guangdong chemical industry(广东化工),2009,36(9):177-189
[21]QIU Zhongping(邱忠平),MAO Canquan(茆灿泉),LIU Yuanyue(刘源月),et al.Screening of a Bioflocculant Producing Strain and its Flocculation Characteristics[J].Chinese Journal of Environmental Engineering(环境工程学报),2009,3(7):1185-1188