Effect of diosgenin on expression of Th17 cell in collageninduced arthritis mice in vitro
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- 英文论文题名：Effect of diosgenin on expression of Th17 cell in collageninduced arthritis mice in vitro
- 论文作者：Yaxian Gao
- 英文论文作者：Yaxian Gao ; Chengde Medical Colledge
- 年：2016
- 作者机构：Chengde Medical College;
- 英文论文关键词：collagen-induced arthritis ; type Ⅱ collagen ; diosgenin ; T cell subsets ; flow cy
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R285.5
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1335k
- 原文格式：D
- 会议级别：全国
摘要
Rheumatoid is a common disease with high disability rate and difficult to cure.The pathogenesis of RA is very complex and not fully understand as yet. Objective: This research is to discuss the effects of diosgenin on expression of Th17 cell in vitro. Methods: The 75 DBA1/J mice was immunized to establish the CIA mouse model, then excise the inguinal lymph nodes of the successful CIA mice model under aseptic condition,made it into single cell suspension and test drug toxicity in order to determine medical concentration with CCK-8. The experiment were divided into five groups including control group, model group, diosgenin high dose, medium dose, low dose group. The percentage of Th17 cells were detected byflow cytometry. Result: According to the results of inhibition rates, determine the dose of diosgenin in the high, medium, low dose groups were 6.25, 12.5, 25 μmol/L respectively. Flow cytometry shows: compared with the control group, the ratio of Th17 cells in model group was significantly higher(P<0.05). Compared with model group, the ratio of Th17 cells in diosgenin high dose group and medium group were significantly decreased(P<0.05), but in diosgenin low group it was no significant difference(P>0.05), Compared with the diosgenin low dose group, the ratio of Th17 cells in diosgenin high dose group and diosgenin medium dose group were reduced(P<0.05), Compared with the diosgenin medium dose group, the ratio of Th17 cells in diosgenin high dose group was no significant difference(P>0.05).Conclusion: Diosgenin had a obvious inhibition on Th17 cells of CIA model mice in vitro.
Rheumatoid is a common disease with high disability rate and difficult to cure.The pathogenesis of RA is very complex and not fully understand as yet. Objective: This research is to discuss the effects of diosgenin on expression of Th17 cell in vitro. Methods: The 75 DBA1/J mice was immunized to establish the CIA mouse model, then excise the inguinal lymph nodes of the successful CIA mice model under aseptic condition,made it into single cell suspension and test drug toxicity in order to determine medical concentration with CCK-8. The experiment were divided into five groups including control group, model group, diosgenin high dose, medium dose, low dose group. The percentage of Th17 cells were detected byflow cytometry. Result: According to the results of inhibition rates, determine the dose of diosgenin in the high, medium, low dose groups were 6.25, 12.5, 25 μmol/L respectively. Flow cytometry shows: compared with the control group, the ratio of Th17 cells in model group was significantly higher(P<0.05). Compared with model group, the ratio of Th17 cells in diosgenin high dose group and medium group were significantly decreased(P<0.05), but in diosgenin low group it was no significant difference(P>0.05), Compared with the diosgenin low dose group, the ratio of Th17 cells in diosgenin high dose group and diosgenin medium dose group were reduced(P<0.05), Compared with the diosgenin medium dose group, the ratio of Th17 cells in diosgenin high dose group was no significant difference(P>0.05).Conclusion: Diosgenin had a obvious inhibition on Th17 cells of CIA model mice in vitro.
Rheumatoid is a common disease with high disability rate and difficult to cure.The pathogenesis of RA is very complex and not fully understand as yet. Objective: This research is to discuss the effects of diosgenin on expression of Th17 cell in vitro. Methods: The 75 DBA1/J mice was immunized to establish the CIA mouse model, then excise the inguinal lymph nodes of the successful CIA mice model under aseptic condition,made it into single cell suspension and test drug toxicity in order to determine medical concentration with CCK-8. The experiment were divided into five groups including control group, model group, diosgenin high dose, medium dose, low dose group. The percentage of Th17 cells were detected byflow cytometry. Result: According to the results of inhibition rates, determine the dose of diosgenin in the high, medium, low dose groups were 6.25, 12.5, 25 μmol/L respectively. Flow cytometry shows: compared with the control group, the ratio of Th17 cells in model group was significantly higher(P<0.05). Compared with model group, the ratio of Th17 cells in diosgenin high dose group and medium group were significantly decreased(P<0.05), but in diosgenin low group it was no significant difference(P>0.05), Compared with the diosgenin low dose group, the ratio of Th17 cells in diosgenin high dose group and diosgenin medium dose group were reduced(P<0.05), Compared with the diosgenin medium dose group, the ratio of Th17 cells in diosgenin high dose group was no significant difference(P>0.05).Conclusion: Diosgenin had a obvious inhibition on Th17 cells of CIA model mice in vitro.
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