A senescence-associated Hevea brasiliensis LEA3 gene is regulated by multiplicate hormones and stresses
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- 英文论文题名：A senescence-associated Hevea brasiliensis LEA3 gene is regulated by multiplicate hormones and stresses
- 论文作者：Zhi Zou ; Xue-mei Dai ; Lifu Yang ; Feng An ; Guishui Xie
- 英文论文作者：Zhi Zou ; Xue-mei Dai ; Lifu Yang ; Feng An ; Guishui Xie ; Danzhou Investigation & Experiment Station of Tropical Crops ; Ministry of Agriculture ; Rubber Research Institute ; Chinese Academy of Tropical Agricultural Sciences
- 年：2016
- 作者机构：Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences;
- 英文论文关键词：Rubber tree(Hevea brasiliensis) ; late embryogenesis abundant ; LEA3 ; leaf development ; leaf senescence ; abiotic stress ; hormone
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S794.1;Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：842k
- 原文格式：D
- 会议级别：全国
摘要
Late embryogenesis abundant(LEA) proteins are a class of small proteins closely associated with the desiccation tolerance in organisms. In this study,an LEA gene(Hb LEA3-1) was isolated from the rubber tree senescent leaves. The 972-bp full-length c DNA contains an ORF putatively encoding a polypeptide of 100 amino acids with a calculated molecular weight of 11.61 k Da and an isolectric point of 9.07. Composed of a hydrophobic N-terminal and a hydrophilic C-terminal and with a grand average of hydrophilicity of-0.498,the protein is predicted to contain an LEA_3 domain and a transit peptide for chloroplast localization. The search of the rubber tree genome suggested the presence of another copy,but no evidence supported its expression. q RT-PCR analysis indicated that Hb LEA3-1 is mainly expressed in leaves with a steadily increasing pattern during leaf development except for a transient decline in early-senescent leaves. Furthermore,the gene expression is regulated by several senescence-inducers such as cold,PEG,NaCl,H_2O_2,ABA,ethylene,Me JA and SA. Our results suggest a role of Hb LEA3-1 in hormone and abiotic stress responses as well as leaf development.
Late embryogenesis abundant(LEA) proteins are a class of small proteins closely associated with the desiccation tolerance in organisms. In this study,an LEA gene(Hb LEA3-1) was isolated from the rubber tree senescent leaves. The 972-bp full-length c DNA contains an ORF putatively encoding a polypeptide of 100 amino acids with a calculated molecular weight of 11.61 k Da and an isolectric point of 9.07. Composed of a hydrophobic N-terminal and a hydrophilic C-terminal and with a grand average of hydrophilicity of-0.498,the protein is predicted to contain an LEA_3 domain and a transit peptide for chloroplast localization. The search of the rubber tree genome suggested the presence of another copy,but no evidence supported its expression. q RT-PCR analysis indicated that Hb LEA3-1 is mainly expressed in leaves with a steadily increasing pattern during leaf development except for a transient decline in early-senescent leaves. Furthermore,the gene expression is regulated by several senescence-inducers such as cold,PEG,NaCl,H_2O_2,ABA,ethylene,Me JA and SA. Our results suggest a role of Hb LEA3-1 in hormone and abiotic stress responses as well as leaf development.
Late embryogenesis abundant(LEA) proteins are a class of small proteins closely associated with the desiccation tolerance in organisms. In this study,an LEA gene(Hb LEA3-1) was isolated from the rubber tree senescent leaves. The 972-bp full-length c DNA contains an ORF putatively encoding a polypeptide of 100 amino acids with a calculated molecular weight of 11.61 k Da and an isolectric point of 9.07. Composed of a hydrophobic N-terminal and a hydrophilic C-terminal and with a grand average of hydrophilicity of-0.498,the protein is predicted to contain an LEA_3 domain and a transit peptide for chloroplast localization. The search of the rubber tree genome suggested the presence of another copy,but no evidence supported its expression. q RT-PCR analysis indicated that Hb LEA3-1 is mainly expressed in leaves with a steadily increasing pattern during leaf development except for a transient decline in early-senescent leaves. Furthermore,the gene expression is regulated by several senescence-inducers such as cold,PEG,NaCl,H_2O_2,ABA,ethylene,Me JA and SA. Our results suggest a role of Hb LEA3-1 in hormone and abiotic stress responses as well as leaf development.
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