Infusion of endothelial progenitor cells ameliorates liver injury in mice after hematopoietic stem cell transplantation
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- 英文论文题名：Infusion of endothelial progenitor cells ameliorates liver injury in mice after hematopoietic stem cell transplantation
- 论文作者：Zeng Lingyu ; Qiao Jianlin ; Wu Yulu ; Li Mingfeng ; Li Wen ; Ding Lan ; Xu Kailin
- 英文论文作者：Zeng Lingyu ; Qiao Jianlin ; Wu Yulu ; Li Mingfeng ; Li Wen ; Ding Lan ; Xu Kailin ; Department of Hematology ; the Affiliated Hospital of Xuzhou Medical University
- 年：2016
- 作者机构：Department of Hematology,the Affiliated Hospital of Xuzhou Medical University;
- 英文论文关键词：Endothelial progenitor cells ; haematopoietic stem cell transplantation ; liver injury ; liver sinusoid
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R575;R457.7
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1262k
- 原文格式：D
- 会议级别：全国
摘要
Aims: Toevaluate the effects of EPCs on liver injury in mice after HSCT. Methods: Mice received HSCT without or with EPCs infusion(HSCT+EPCs). Untreated mice were used as control. Liver and whole blood were collected post HSCT and used for the analysis of pathology of liver sinusoidal endothelial cells(LSECs) and hepatocytes, liver ultrastructure, function, level of IL-6, TNF-a and platelet activation. Results: Severe LSECs injury,hepatocyte damage, abnormal liver function was observed in HSCT group. In addition, increased P-selectin expression and secretion of IL-6, TNF-a was also found. However, all the above changes were alleviated in HSCT+EPCs at all the time points and normalized at the endpoint. Meanwhile, EPCs-induced repair of LSECs and hepatocytes was totally inhibited by the addition of anti-VE-cadherin antibody.Conclusions: EPCs infusion ameliorated the damage to LSECs and hepatocytes as well asreducedsecretion of IL-6, TNF-a and inhibited platelet activation after HSCT, leading to improved liver function, suggesting EPCsmight be a new therapeutic strategy in the prophylaxis of liver injury after HSCT.
Aims: Toevaluate the effects of EPCs on liver injury in mice after HSCT. Methods: Mice received HSCT without or with EPCs infusion(HSCT+EPCs). Untreated mice were used as control. Liver and whole blood were collected post HSCT and used for the analysis of pathology of liver sinusoidal endothelial cells(LSECs) and hepatocytes, liver ultrastructure, function, level of IL-6, TNF-a and platelet activation. Results: Severe LSECs injury,hepatocyte damage, abnormal liver function was observed in HSCT group. In addition, increased P-selectin expression and secretion of IL-6, TNF-a was also found. However, all the above changes were alleviated in HSCT+EPCs at all the time points and normalized at the endpoint. Meanwhile, EPCs-induced repair of LSECs and hepatocytes was totally inhibited by the addition of anti-VE-cadherin antibody.Conclusions: EPCs infusion ameliorated the damage to LSECs and hepatocytes as well asreducedsecretion of IL-6, TNF-a and inhibited platelet activation after HSCT, leading to improved liver function, suggesting EPCsmight be a new therapeutic strategy in the prophylaxis of liver injury after HSCT.
Aims: Toevaluate the effects of EPCs on liver injury in mice after HSCT. Methods: Mice received HSCT without or with EPCs infusion(HSCT+EPCs). Untreated mice were used as control. Liver and whole blood were collected post HSCT and used for the analysis of pathology of liver sinusoidal endothelial cells(LSECs) and hepatocytes, liver ultrastructure, function, level of IL-6, TNF-a and platelet activation. Results: Severe LSECs injury,hepatocyte damage, abnormal liver function was observed in HSCT group. In addition, increased P-selectin expression and secretion of IL-6, TNF-a was also found. However, all the above changes were alleviated in HSCT+EPCs at all the time points and normalized at the endpoint. Meanwhile, EPCs-induced repair of LSECs and hepatocytes was totally inhibited by the addition of anti-VE-cadherin antibody.Conclusions: EPCs infusion ameliorated the damage to LSECs and hepatocytes as well asreducedsecretion of IL-6, TNF-a and inhibited platelet activation after HSCT, leading to improved liver function, suggesting EPCsmight be a new therapeutic strategy in the prophylaxis of liver injury after HSCT.
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