Development of New EST-SSR Markers of Wheat Leaf Rust Based on Puccinia triticina Transcriptome
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- 英文论文题名：Development of New EST-SSR Markers of Wheat Leaf Rust Based on Puccinia triticina Transcriptome
- 论文作者：YANG Wen-xiang ; WANG Zhi ; MENG Qing-fang ; LIU Da-qun
- 英文论文作者：YANG Wen-xiang ; WANG Zhi ; MENG Qing-fang ; LIU Da-qun ; Department of Plant Pathology ; Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas
- 年：2016
- 作者机构：Department of Plant Pathology,Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas;
- 英文论文关键词：Wheat leaf rust ; Transcriptome ; EST-SSR molecular marker ; Polymorphism
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.121.43
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：52k
- 原文格式：O
- 会议级别：全国
摘要
Wheat leaf rust caused by Puccinia triticina has different degree of threat to wheat production in the important wheat diseases in almost all wheat producing areas in the world.Due to the variation of wheat leaf rust and the same disease resistant varieties of single plant,pathogen is constantly changing.Pathogen monitoring of virulence trend and population polymorphism are important task of the wheat leaf rust population dynamic transformation research and virulence polymorphism research.Development of new molecular markers of P.triticina can make us deeply understand the molecular pathogenetic mechanism,survay virulence and control wheat leaf rust.At present,only 31 pairs of wheat leaf rust EST-SSR markers have been reported,so it is an urgent need to develop new EST-SSR perimers.The objective of this research was to develop new polymorphic EST-SSR markers of wheat leaf rust based on transcriptome sequencing for reveal the genetic relationship and genetic background.We used Illumina Hiseq 2000 to assemble the transcriptomes and then we got 46 008 Unigenes.MISA software was then used to screen the EST-SSR molecular markers from these and 3 085 unigenes containing 3 729 EST-SSR loci were obtained.A total of 3 065 EST-SSR primers on 613 loci were designed by using Primer3 software and 180 of these were synthesized randomly.Among them 141 pairs of the primers could be amplified in P.triticina,and 37 of these produced polymorphic bands which can be applied to detect the genetic diversity in a natural population of P.triticina.The total allele number was 85,with average allele number of 2.3 per locus.Then we used the 37 EST-SSRs to judge the polymorphism of different 48 stains of P.triticina collected from Hebei province and Xinjiang.The results showed that most of the P.triticina stains in the two regions were clustered into different groups which indicated that the EST-SSR polymorphism and geographical distribution of P.triticina stains have certain correlation.
Wheat leaf rust caused by Puccinia triticina has different degree of threat to wheat production in the important wheat diseases in almost all wheat producing areas in the world.Due to the variation of wheat leaf rust and the same disease resistant varieties of single plant,pathogen is constantly changing.Pathogen monitoring of virulence trend and population polymorphism are important task of the wheat leaf rust population dynamic transformation research and virulence polymorphism research.Development of new molecular markers of P.triticina can make us deeply understand the molecular pathogenetic mechanism,survay virulence and control wheat leaf rust.At present,only 31 pairs of wheat leaf rust EST-SSR markers have been reported,so it is an urgent need to develop new EST-SSR perimers.The objective of this research was to develop new polymorphic EST-SSR markers of wheat leaf rust based on transcriptome sequencing for reveal the genetic relationship and genetic background.We used Illumina Hiseq 2000 to assemble the transcriptomes and then we got 46 008 Unigenes.MISA software was then used to screen the EST-SSR molecular markers from these and 3 085 unigenes containing 3 729 EST-SSR loci were obtained.A total of 3 065 EST-SSR primers on 613 loci were designed by using Primer3 software and 180 of these were synthesized randomly.Among them 141 pairs of the primers could be amplified in P.triticina,and 37 of these produced polymorphic bands which can be applied to detect the genetic diversity in a natural population of P.triticina.The total allele number was 85,with average allele number of 2.3 per locus.Then we used the 37 EST-SSRs to judge the polymorphism of different 48 stains of P.triticina collected from Hebei province and Xinjiang.The results showed that most of the P.triticina stains in the two regions were clustered into different groups which indicated that the EST-SSR polymorphism and geographical distribution of P.triticina stains have certain correlation.
Wheat leaf rust caused by Puccinia triticina has different degree of threat to wheat production in the important wheat diseases in almost all wheat producing areas in the world.Due to the variation of wheat leaf rust and the same disease resistant varieties of single plant,pathogen is constantly changing.Pathogen monitoring of virulence trend and population polymorphism are important task of the wheat leaf rust population dynamic transformation research and virulence polymorphism research.Development of new molecular markers of P.triticina can make us deeply understand the molecular pathogenetic mechanism,survay virulence and control wheat leaf rust.At present,only 31 pairs of wheat leaf rust EST-SSR markers have been reported,so it is an urgent need to develop new EST-SSR perimers.The objective of this research was to develop new polymorphic EST-SSR markers of wheat leaf rust based on transcriptome sequencing for reveal the genetic relationship and genetic background.We used Illumina Hiseq 2000 to assemble the transcriptomes and then we got 46 008 Unigenes.MISA software was then used to screen the EST-SSR molecular markers from these and 3 085 unigenes containing 3 729 EST-SSR loci were obtained.A total of 3 065 EST-SSR primers on 613 loci were designed by using Primer3 software and 180 of these were synthesized randomly.Among them 141 pairs of the primers could be amplified in P.triticina,and 37 of these produced polymorphic bands which can be applied to detect the genetic diversity in a natural population of P.triticina.The total allele number was 85,with average allele number of 2.3 per locus.Then we used the 37 EST-SSRs to judge the polymorphism of different 48 stains of P.triticina collected from Hebei province and Xinjiang.The results showed that most of the P.triticina stains in the two regions were clustered into different groups which indicated that the EST-SSR polymorphism and geographical distribution of P.triticina stains have certain correlation.
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