全细胞催化芦丁水解制备异槲皮素的研究
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- 英文论文题名：Application of whole cell catalysis in rutin hydrolysis for isoquercetin production
- 论文作者：唐诗潮 ; 李晓凤
- 英文论文作者：Shichao Tang ; Xiaofeng Li ; South China University of Technology ; College of Food Sciences and technology
- 年：2016
- 作者机构：华南理工大学食品科学与工程学院;
- 论文关键词：芦丁 ; 异槲皮素 ; 黑曲霉 ; 酶转化 ; 诱导
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十三分会：绿色化学
- 语种：中文
- 分类号：TQ28
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十三分会 ; 绿色化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：136k
- 原文格式：D
- 会议级别：全国
摘要
异槲皮素是广泛存在于药用植物、食品饮料、蔬菜水果中的天然黄酮类化合物,具有多种重要的生理活性及多方面优良的药理活性。目前其合成主要通过化学法和酶法进行。本研究探讨了基于全细胞生物催化的新型制备方法,通过催化芦丁水解制备获得了高纯度的异槲皮素。研究分析了全细胞催化剂培养制备过程中,不同的诱导条件对多种细菌和真菌细胞水解酶产酶活性的影响。研究结果表明,黑曲霉全细胞催化剂具有优异的催化活性和高区域选择性(99%);与纯酶法相比,全细胞催化法大大减少了繁琐的酶制备提纯工艺,降低了生产成本。本研究为异槲皮素及其相关结构化合物的高效制备提供了一条绿色、高效的新途径。
Isoquercetin is widely present in medicinal plants, food and beverage, natural flavonoids in fruits and vegetables, with pharmacological activity and physiological activity of a variety of important and excellent in many aspects. At present, the main synthesis by chemical and enzymatic method. The new preparation method was discussed in this paper based on whole cell biocatalysis the catalytic hydrolysis of rutin, Isoquercitrin was obtained with high purity. Research and analysis of the whole cell catalyst to produce in the preparation process, different induction conditions of A variety of bacteria and fungi Cell effect of hydrolytic enzyme production activity. The results indicated that the Aspergillus niger whole cell catalyst has excellent catalytic activity and regional high selectivity(99%); compared with the method of pure enzyme, whole cell catalysis greatly reduces the complex of the enzyme, purification process, reduce the production cost. This study for Isoquercitrin and related structured complexes of high preparation provides a green and efficient new way.
Isoquercetin is widely present in medicinal plants, food and beverage, natural flavonoids in fruits and vegetables, with pharmacological activity and physiological activity of a variety of important and excellent in many aspects. At present, the main synthesis by chemical and enzymatic method. The new preparation method was discussed in this paper based on whole cell biocatalysis the catalytic hydrolysis of rutin, Isoquercitrin was obtained with high purity. Research and analysis of the whole cell catalyst to produce in the preparation process, different induction conditions of A variety of bacteria and fungi Cell effect of hydrolytic enzyme production activity. The results indicated that the Aspergillus niger whole cell catalyst has excellent catalytic activity and regional high selectivity(99%); compared with the method of pure enzyme, whole cell catalysis greatly reduces the complex of the enzyme, purification process, reduce the production cost. This study for Isoquercitrin and related structured complexes of high preparation provides a green and efficient new way.
引文
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[2]Hara S,Morita R,Ogawa T,et al.Experimental and Toxicologic Pathology,2014,66(5):225-234.
[3]吴迪.芦丁鼠李糖苷酶的酶性质及异槲皮苷的纯化精制[D].大连工业大学,2012.
[4]王秋月.高效异槲皮素产生菌的选育及转化条件优化[D].河北大学,2015.
[2]Hara S,Morita R,Ogawa T,et al.Experimental and Toxicologic Pathology,2014,66(5):225-234.
[3]吴迪.芦丁鼠李糖苷酶的酶性质及异槲皮苷的纯化精制[D].大连工业大学,2012.
[4]王秋月.高效异槲皮素产生菌的选育及转化条件优化[D].河北大学,2015.