三倍体芍药品种花粉育性研究
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- 英文论文题名：Studies on Pollen Fertility of Triploid Herbaceous Peony Cultivars
- 论文作者：王玉蛟 ; 刘建鑫 ; 于晓南
- 英文论文作者：WANG Yu-jiao ; LIU Jian-xin ; YU Xiao-nan ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University
- 年：2016
- 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;
- 论文关键词：芍药 ; 三倍体 ; 花粉活力 ; 花粉萌发
- 英文论文关键词：Herbaceous peony ; Triploid ; Pollen viability ; Pollen germination
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.12
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：666k
- 原文格式：O
- 会议级别：全国
摘要
为了充分了解芍药三倍体品种的育种潜力,促进我国芍药新品种培育工作的发展,本研究选取了3个芍药三倍体新品种‘Brightness',‘Roseletter'以及‘Coral Sunset',用I_2-KI染色法分别对其花粉活力进行了测定;并以国内二倍体品种‘粉玉奴'为母本,利用荧光显微技术,观察了三倍体品种在其柱头上的花粉萌发和花粉管生长情况。结果发现:①所观察的三倍体品种的花粉活力均非常低,其中品种‘Brightness'的花粉活力最高,为6.7%;品种‘Coral Sunset'的花粉活力最低,为2.2%。②所选三倍体品种的花粉在异种柱头上只有极少数的萌发,且花粉管极短,柱头乳突细胞及花粉管内出现大量胼胝质,未观察到花粉管伸入进花柱,同时发现大量花粉破裂及从柱头上脱落,组内远缘杂交亲和性非常差。
In order to fully understand the breeding potential of triploid herbaceous peony cultivars and promote the development of cultivating more new peony cultivars in China,3 triploid cultivars,' Brightness',' Roseletter' and ' Coral Sunset'were selected to investigate their pollen viability and fertility.Pollen viability was determined by I2-KI.Meanwhile,with the domestic diploid cultivar ' Fen Yu Nu' as female parent,the pollen germination and the pollen tube growth on its stigma were observed by fluorescence microscopy.The results showed:①The pollen viability of triploid cultivars were very low.Pollen viability of ' Brightness' was the best with 6.7%,while the ' Coral Sunset' s was the lowest with 2.2%.②The pollen grains of triploid cultivars had a very low germination rate on the maternal stigma.The pollen tubes were very short and couldn't enter the stigma,a lot of callose was found in papillose cells of stigma and pollen tubes.Meanwhile,a large number of pollen broke and detached from the stigma.The compatibility of distant hybridization was very low.
In order to fully understand the breeding potential of triploid herbaceous peony cultivars and promote the development of cultivating more new peony cultivars in China,3 triploid cultivars,' Brightness',' Roseletter' and ' Coral Sunset'were selected to investigate their pollen viability and fertility.Pollen viability was determined by I2-KI.Meanwhile,with the domestic diploid cultivar ' Fen Yu Nu' as female parent,the pollen germination and the pollen tube growth on its stigma were observed by fluorescence microscopy.The results showed:①The pollen viability of triploid cultivars were very low.Pollen viability of ' Brightness' was the best with 6.7%,while the ' Coral Sunset' s was the lowest with 2.2%.②The pollen grains of triploid cultivars had a very low germination rate on the maternal stigma.The pollen tubes were very short and couldn't enter the stigma,a lot of callose was found in papillose cells of stigma and pollen tubes.Meanwhile,a large number of pollen broke and detached from the stigma.The compatibility of distant hybridization was very low.
引文
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14.王历慧,于晓南,郑黎文.2011.中西方芍药切花应用与市场趋势分析[J].黑龙江农业科学,(2):147-149.
15.王越岚.2009.牡丹的杂交育种及组问杂种育性的研究[D].北京:北京林业大学.
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18.于晓南,宋焕芝,郑黎文.2010.国外观赏芍药育种与应用及其启示[J].湖南农业大学学报(自然科学版),36(2):159-162.
19.袁国良.2014.用三倍体做母本实现百合种质渗入育种[D].浙江:浙江大学.
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21.Zenkteler M.1990.Invitro fertilization of ovules of some species of Brassicaceae[J].Plant Breeding,105:221-228.
2.邓衍明,叶晓青.2012.园艺作物远缘杂交受精前生殖障碍及其克服方法[J].华北农学报,S1:81-86.
3.盖伟玲,盖树鹏,郑国生.2011.牡丹新鲜花粉活力的快速测定[J].育苗技术,5:32-34.
4.郝津藜,赵娜,石颜通,等.2014.黄牡丹远缘杂交亲和性及杂交后代形态分析[J].园艺学报,8:1651-1662.
5.郝青,刘政安,舒庆艳,等.2008.中国首例芍药牡丹远缘杂交种的发现及鉴定[J].园艺学报,2008,06:853-858.
6.韩成刚,盖树鹏.2012.芍药花粉活力测定方法的研究[J].江苏农业科学,5:124-126.
7.康向阳,朱之悌,张志毅.1999毛白杨异源三倍体形态和减数分裂观察[J].北京林业大学学报,21(1).
8.Kho Y O,Baer J.1968.Observing pollen tubes by means of florescence[J].Euphytica,17(2):298-302.
9.雷家军,梁印.2012.百合三倍体种间杂种花粉母细胞减数分裂行为观察[J].吉林农业大学学报,34(2):162-165.
10.李克虎.2011.异源三倍体百合(Lilium)育种潜力研究[D].浙江:浙江大学.
11.律春燕,王雁,朱向涛,等.2009.黄牡丹与芍药组间杂交花粉与柱头识别的解剖学研究[J].西北植物学报,10:1988-1994..
12.Van Tuyl J M.1997.Interspecific hybridization of flower bulbs;a review[J].Breeding Genetics and Selection,465-475.
13.王爱云,李枸,胡大有.2006.诸葛菜与芸薹属间花粉与柱头相互作用的研究[J].湖南农业大学学报(自然科学版),32(3):232-236.
14.王历慧,于晓南,郑黎文.2011.中西方芍药切花应用与市场趋势分析[J].黑龙江农业科学,(2):147-149.
15.王越岚.2009.牡丹的杂交育种及组问杂种育性的研究[D].北京:北京林业大学.
16.王忠.2000.植物生理学[M].北京:中国农业出版社.
17.杨涛,宋丹,张晓莹,等.2015.部分蔷薇属植物远缘杂交亲和性评价[J].东北农业大学学报,2:72-77.
18.于晓南,宋焕芝,郑黎文.2010.国外观赏芍药育种与应用及其启示[J].湖南农业大学学报(自然科学版),36(2):159-162.
19.袁国良.2014.用三倍体做母本实现百合种质渗入育种[D].浙江:浙江大学.
20.袁艳波.2014.芍药属远缘品种引种与杂交育种研究[D].北京:北京林业大学.
21.Zenkteler M.1990.Invitro fertilization of ovules of some species of Brassicaceae[J].Plant Breeding,105:221-228.