Establishment and Gene Expression Characteristics of the Silk Gland Cell Line BmSG-SWU1 of Bombyx mori(Lepidoptera: Bombycidae)
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- 英文论文题名：Establishment and Gene Expression Characteristics of the Silk Gland Cell Line BmSG-SWU1 of Bombyx mori(Lepidoptera: Bombycidae)
- 论文作者：Zhen-yue Feng ; Chun Pan ; Min Liu ; Zhi-qiang Tian ; Xue-mei Chen ; Xiu-xiu Kuang ; Cheng Lu ; Min-hui Pan
- 英文论文作者：Zhen-yue Feng ; Chun Pan ; Min Liu ; Zhi-qiang Tian ; Xue-mei Chen ; Xiu-xiu Kuang ; Cheng Lu ; Min-hui Pan ; State Key Laboratory of Silkworm Genome Biology ; Institute of Sericulture and Systems Biology ; Southwest University ; Institute of Life Science Technology ; Heilongjiang Bayi Agriculture University
- 年：2016
- 作者机构：State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest University;Institute of Life Science Technology, Heilongjiang Bayi Agriculture University;
- 英文论文关键词：Bombyx mori ; silk gland ; cell line ; gene expression
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：499k
- 原文格式：D
- 会议级别：全国
摘要
A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori L. larvae by performing primary cultures for 2 yr. The cell line was comprised of long and thin shuttle cells. The percentage of cells with the chromosome number 2n=56 was 76.28%. Therefore, the cell line was considered a diploid cell line. Fingerprint analysis of BmSG-SWU1 cells and four other cell lines indicated that BmSG-SWU1 cells had a specific fingerprint, and that the genetic relationship between BmSG-SWU1 cells and the original silk gland tissue was the closest. We used whole genome microarray data, reverse transcription-polymerase chain reaction and fluorescence quantitative real-time-polymerase chain reaction to analyze the expression profile of BmSG-SWU1 cells. On day 3 of the fifth instar silk gland, the two known silk gland-specific genes Fib-L and P25 as well as 18 other highly expressed genes were found in the whole genome microarray data. Only some of these were expressed in the BmSG-SWU1 cell line, which showed that this cell line had maintained some gene expression characteristics of silk gland. The key cell cycle-control genes BmCyclin B and BmCyclin B3 of the G2 and M phase were expressed at low levels in silk glands. Contrarily, they were highly expressed in BmSG-SWU1 cells, in the ovaries and other cell lines, which suggested that BmCyclin B and BmCyclin B3 are important for the transformation of BmSG-SWU1 cell line from endoreduplication to the mitotic cycle.
A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori L. larvae by performing primary cultures for 2 yr. The cell line was comprised of long and thin shuttle cells. The percentage of cells with the chromosome number 2n=56 was 76.28%. Therefore, the cell line was considered a diploid cell line. Fingerprint analysis of BmSG-SWU1 cells and four other cell lines indicated that BmSG-SWU1 cells had a specific fingerprint, and that the genetic relationship between BmSG-SWU1 cells and the original silk gland tissue was the closest. We used whole genome microarray data, reverse transcription-polymerase chain reaction and fluorescence quantitative real-time-polymerase chain reaction to analyze the expression profile of BmSG-SWU1 cells. On day 3 of the fifth instar silk gland, the two known silk gland-specific genes Fib-L and P25 as well as 18 other highly expressed genes were found in the whole genome microarray data. Only some of these were expressed in the BmSG-SWU1 cell line, which showed that this cell line had maintained some gene expression characteristics of silk gland. The key cell cycle-control genes BmCyclin B and BmCyclin B3 of the G2 and M phase were expressed at low levels in silk glands. Contrarily, they were highly expressed in BmSG-SWU1 cells, in the ovaries and other cell lines, which suggested that BmCyclin B and BmCyclin B3 are important for the transformation of BmSG-SWU1 cell line from endoreduplication to the mitotic cycle.
A BmSG-SWU1 cell line was established from the silk gland tissues of newly hatched Bombyx mori L. larvae by performing primary cultures for 2 yr. The cell line was comprised of long and thin shuttle cells. The percentage of cells with the chromosome number 2n=56 was 76.28%. Therefore, the cell line was considered a diploid cell line. Fingerprint analysis of BmSG-SWU1 cells and four other cell lines indicated that BmSG-SWU1 cells had a specific fingerprint, and that the genetic relationship between BmSG-SWU1 cells and the original silk gland tissue was the closest. We used whole genome microarray data, reverse transcription-polymerase chain reaction and fluorescence quantitative real-time-polymerase chain reaction to analyze the expression profile of BmSG-SWU1 cells. On day 3 of the fifth instar silk gland, the two known silk gland-specific genes Fib-L and P25 as well as 18 other highly expressed genes were found in the whole genome microarray data. Only some of these were expressed in the BmSG-SWU1 cell line, which showed that this cell line had maintained some gene expression characteristics of silk gland. The key cell cycle-control genes BmCyclin B and BmCyclin B3 of the G2 and M phase were expressed at low levels in silk glands. Contrarily, they were highly expressed in BmSG-SWU1 cells, in the ovaries and other cell lines, which suggested that BmCyclin B and BmCyclin B3 are important for the transformation of BmSG-SWU1 cell line from endoreduplication to the mitotic cycle.
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