Dynamics of the CD4+ T cell subsets and cytokine profiles during Trichinella spiralis infection in BALB/c mice
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- 英文论文题名：Dynamics of the CD4+ T cell subsets and cytokine profiles during Trichinella spiralis infection in BALB/c mice
- 论文作者：Kai Guo ; Ximeng Sun ; Xiaohuan Wang ; Yuan Gu ; Yuli Cheng ; Jing Yang ; Xinping Zhu
- 英文论文作者：Kai Guo ; Ximeng Sun ; Xiaohuan Wang ; Yuan Gu ; Yuli Cheng ; Jing Yang ; Xinping Zhu ; Department of Parasitology ; School of Basic Medical Sciences ; Capital Medical University
- 年：2016
- 作者机构：Department of Parasitology, School of Basic Medical Sciences, Capital Medical University;
- 英文论文关键词：Trichinella spiralis infection ; CD4~+ T cell ; regulatory T cells ; FOXP3 gene
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R532.14
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：158k
- 原文格式：D
- 会议级别：全国
摘要
Objectives Trichinella spiralis(T. spiralis) is an intracellular pathogen in skeletal muscles of the host. Trichinellosis is a serious zoonotic parasitic disease resulting from infection by T. spiralis, and infects humans in 55 countries around the world. During T. spiralis infection, the entire life cycle is completed in the same host(Intestinal phase, NBL migration, Larva capsule, Convalescent phase). To study the impacts of Trichinella spiralis experimental infection on the polarization of CD4~+ T cells, an attempt was undertaken to detect CD4~+ T cell subsets, corresponding cytokine profiles, especially the pathway of regulatory T cells production during different T. spiralis infection phases. Methods BALB/c mice were each infected with 400 infective T. spiralis muscle larvae by oral gavage, meanwhile, uninfected group as control. After 6 days, 15 days, 30 days, 60 days T. spiralis infection, FACS was used to determine the proportion of Th1(CD3~+CD4~+IFN-γ~+), Th2(CD3~+CD4~+IL-4~+), Th17(CD3~+CD4~+IL-17A~+) cells and regulatory T cells(Tregs) in spleen cells. Simultaneously, cytokines secretion(IL-21, IL-17 A, IL-4, IFN-γ, TGF-β, IL12/23p40, IL-10, IL-6) were detected by ELISA. TGF-β, CD28, IL-2, TCR pathways of FOXP3 gene were preliminarily determined by western blot and real-time PCR. Results After day 6 T. spiralis infection, Th1, Th2 cells and Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) were elevated by FACS analysis. The increased percentage of Th2 cells and Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) in CD4~+ T cells were observed, but not Th1 cells after 15 days post-infection. However, there was no significantly change that proportion of Th1, Th2 cells and Tregs after 30 and 60 days post-infection. It was notable that Th17 cells were not significantly promoted in the entire infection phase. Cytokine data demonstrated that the levels of TGF-β, IL-4 and IL-21 were elevated at day 6 or 15 post-infection, the secretions of IL-6 and IL-10 were enhanced at day 6, 15 or 60 post-infection, IL12/23p40 was elevated at day 15 post-infection, compared with uninfected group. Meanwhile, compared to control group, the level of TGF-β at day 60 post-infection, the secretions of IFN-γ and IL12/23p40 at day 30 post-infection, and the secretion of IL-17 A at day 15 and 30 post-infection were suppressed. In addition, we found that the western blot data revealed that expression of CREB and NFAT1, phosphorated-smad2 and smad3 were increased in the infected group. Conclusions It is a mix Th1/Th2 response in intestinal phase of T. spiralis infection, while Th2 response is primary in NBL migration. Moreover, Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) were induced by T. spiralis infection in the two phases(Intestinal phase, NBL migration). TCR and TGF-β pathways could be important to expression of FOXP3 gene during T. spiralis infection.
Objectives Trichinella spiralis(T. spiralis) is an intracellular pathogen in skeletal muscles of the host. Trichinellosis is a serious zoonotic parasitic disease resulting from infection by T. spiralis, and infects humans in 55 countries around the world. During T. spiralis infection, the entire life cycle is completed in the same host(Intestinal phase, NBL migration, Larva capsule, Convalescent phase). To study the impacts of Trichinella spiralis experimental infection on the polarization of CD4~+ T cells, an attempt was undertaken to detect CD4~+ T cell subsets, corresponding cytokine profiles, especially the pathway of regulatory T cells production during different T. spiralis infection phases. Methods BALB/c mice were each infected with 400 infective T. spiralis muscle larvae by oral gavage, meanwhile, uninfected group as control. After 6 days, 15 days, 30 days, 60 days T. spiralis infection, FACS was used to determine the proportion of Th1(CD3~+CD4~+IFN-γ~+), Th2(CD3~+CD4~+IL-4~+), Th17(CD3~+CD4~+IL-17A~+) cells and regulatory T cells(Tregs) in spleen cells. Simultaneously, cytokines secretion(IL-21, IL-17 A, IL-4, IFN-γ, TGF-β, IL12/23p40, IL-10, IL-6) were detected by ELISA. TGF-β, CD28, IL-2, TCR pathways of FOXP3 gene were preliminarily determined by western blot and real-time PCR. Results After day 6 T. spiralis infection, Th1, Th2 cells and Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) were elevated by FACS analysis. The increased percentage of Th2 cells and Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) in CD4~+ T cells were observed, but not Th1 cells after 15 days post-infection. However, there was no significantly change that proportion of Th1, Th2 cells and Tregs after 30 and 60 days post-infection. It was notable that Th17 cells were not significantly promoted in the entire infection phase. Cytokine data demonstrated that the levels of TGF-β, IL-4 and IL-21 were elevated at day 6 or 15 post-infection, the secretions of IL-6 and IL-10 were enhanced at day 6, 15 or 60 post-infection, IL12/23p40 was elevated at day 15 post-infection, compared with uninfected group. Meanwhile, compared to control group, the level of TGF-β at day 60 post-infection, the secretions of IFN-γ and IL12/23p40 at day 30 post-infection, and the secretion of IL-17 A at day 15 and 30 post-infection were suppressed. In addition, we found that the western blot data revealed that expression of CREB and NFAT1, phosphorated-smad2 and smad3 were increased in the infected group. Conclusions It is a mix Th1/Th2 response in intestinal phase of T. spiralis infection, while Th2 response is primary in NBL migration. Moreover, Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) were induced by T. spiralis infection in the two phases(Intestinal phase, NBL migration). TCR and TGF-β pathways could be important to expression of FOXP3 gene during T. spiralis infection.
Objectives Trichinella spiralis(T. spiralis) is an intracellular pathogen in skeletal muscles of the host. Trichinellosis is a serious zoonotic parasitic disease resulting from infection by T. spiralis, and infects humans in 55 countries around the world. During T. spiralis infection, the entire life cycle is completed in the same host(Intestinal phase, NBL migration, Larva capsule, Convalescent phase). To study the impacts of Trichinella spiralis experimental infection on the polarization of CD4~+ T cells, an attempt was undertaken to detect CD4~+ T cell subsets, corresponding cytokine profiles, especially the pathway of regulatory T cells production during different T. spiralis infection phases. Methods BALB/c mice were each infected with 400 infective T. spiralis muscle larvae by oral gavage, meanwhile, uninfected group as control. After 6 days, 15 days, 30 days, 60 days T. spiralis infection, FACS was used to determine the proportion of Th1(CD3~+CD4~+IFN-γ~+), Th2(CD3~+CD4~+IL-4~+), Th17(CD3~+CD4~+IL-17A~+) cells and regulatory T cells(Tregs) in spleen cells. Simultaneously, cytokines secretion(IL-21, IL-17 A, IL-4, IFN-γ, TGF-β, IL12/23p40, IL-10, IL-6) were detected by ELISA. TGF-β, CD28, IL-2, TCR pathways of FOXP3 gene were preliminarily determined by western blot and real-time PCR. Results After day 6 T. spiralis infection, Th1, Th2 cells and Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) were elevated by FACS analysis. The increased percentage of Th2 cells and Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) in CD4~+ T cells were observed, but not Th1 cells after 15 days post-infection. However, there was no significantly change that proportion of Th1, Th2 cells and Tregs after 30 and 60 days post-infection. It was notable that Th17 cells were not significantly promoted in the entire infection phase. Cytokine data demonstrated that the levels of TGF-β, IL-4 and IL-21 were elevated at day 6 or 15 post-infection, the secretions of IL-6 and IL-10 were enhanced at day 6, 15 or 60 post-infection, IL12/23p40 was elevated at day 15 post-infection, compared with uninfected group. Meanwhile, compared to control group, the level of TGF-β at day 60 post-infection, the secretions of IFN-γ and IL12/23p40 at day 30 post-infection, and the secretion of IL-17 A at day 15 and 30 post-infection were suppressed. In addition, we found that the western blot data revealed that expression of CREB and NFAT1, phosphorated-smad2 and smad3 were increased in the infected group. Conclusions It is a mix Th1/Th2 response in intestinal phase of T. spiralis infection, while Th2 response is primary in NBL migration. Moreover, Tregs(CD4~+CD25~+Foxp3~+ and CD4~+CD25-Foxp3~+) were induced by T. spiralis infection in the two phases(Intestinal phase, NBL migration). TCR and TGF-β pathways could be important to expression of FOXP3 gene during T. spiralis infection.
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