尼奥罗非鱼杂种性别类型与性腺发育初步研究
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- 英文论文题名：Gendersand Gonad Development inthe Hybrids of Oreochromisniloticus(♀)×O.aureus(♂)
- 论文作者：赵永华 ; 陈晓武 ; 王燕 ; 魏继海 ; 伞桂宝 ; 简伟业 ; 赵金良
- 英文论文作者：ZHAO Yonghua ; CHEN Xiaowu ; WANG Yan ; WEI Jihai ; SANGuibao ; JIAN Weiye ; ZHAO Jinliang ; Laboratory of Freshwater Fisheries Germplasm Resources ; Ministry of Agriculture ; Shanghai Ocean University ; Guangzhou Panyu Institute of Agricultural Sciences ; Maoming Weiye Tilapia Seed Farm
- 年：2016
- 作者机构：上海海洋大学农业部淡水水产种质资源重点实验室;广州市番禺区农业科学研究所;茂名市伟业罗非鱼良种场;
- 论文关键词：尼奥罗非鱼 ; 性别类型 ; 性腺发育 ; 组织切片
- 英文论文关键词：O.niloticus♀×O.aureus♂ ; gender type ; gonad development ; histologicalmethod
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：626k
- 原文格式：D
- 会议级别：全国
摘要
通过组织学方法,研究了0.5-4.5月龄尼奥罗非鱼杂种(尼罗罗非鱼♀×奥利亚罗非鱼♂)的性腺发育特征与性别类型。结果表明,0.5月龄尼奥罗非鱼原始性腺尚未分化;1月龄,精巢中原始生殖细胞开始分化成精原细胞;2.5月龄出现初级精母细胞;3月龄出现次级精母细胞;3.5-4月龄精巢中存在不同发育阶段的生殖细胞,壶腹空腔中分散着稀疏的精子;4.5月龄精巢壶腹腔内密集大量精子。在2.5、3.5、4、4.5月龄抽样中,均检测到雌鱼,2.5-3.5月龄卵巢,以第Ⅱ时相卵母细胞为主;4-4.5月龄卵巢,以第Ⅲ时相卵母细胞为主。研究结果初步表明,尼奥罗非鱼杂种中观察到雄性、雌性两种性别类型,以雄性性别为主;杂种雌、雄鱼的性腺均能正常发育。
The gender type and gonad developmentof O. niloticus(♀)×O.aureus(♂)at 0.5-4.5monthwere observedby microscopic histological observation. Theresults showed thatthe primordial gonad of O.niloticus(♀)×O.aureus(♂)was undifferentiatedat 0.5-month-old.The primordial germ cells in the testis differentiated into spermatogonia around 1 month, primary spermatocytesoccurred at the age of 2.5 month, while secondary spermatocytes presented around3-month-old. The germ cells presence different developmental stages in the testis and sparse sperm dispersed in ampulla cavity at the age of 3.5-4.5 months,while intensive sperm dispersed in ampulla cavity around 4.5 months. Among the samples, female individuals were also detected at the 2.5,3.5,4,4.5 months, respectively,the oocytes were in phase Ⅱat 2.5- 3.5 monthsovarian,and phase Ⅱat 4, 4.5 month-old.This study suggested thatthere were two kinds of gender existed in hybrids with the male dominated, the testis and ovarian in the hybrids could develop normally.
The gender type and gonad developmentof O. niloticus(♀)×O.aureus(♂)at 0.5-4.5monthwere observedby microscopic histological observation. Theresults showed thatthe primordial gonad of O.niloticus(♀)×O.aureus(♂)was undifferentiatedat 0.5-month-old.The primordial germ cells in the testis differentiated into spermatogonia around 1 month, primary spermatocytesoccurred at the age of 2.5 month, while secondary spermatocytes presented around3-month-old. The germ cells presence different developmental stages in the testis and sparse sperm dispersed in ampulla cavity at the age of 3.5-4.5 months,while intensive sperm dispersed in ampulla cavity around 4.5 months. Among the samples, female individuals were also detected at the 2.5,3.5,4,4.5 months, respectively,the oocytes were in phase Ⅱat 2.5- 3.5 monthsovarian,and phase Ⅱat 4, 4.5 month-old.This study suggested thatthere were two kinds of gender existed in hybrids with the male dominated, the testis and ovarian in the hybrids could develop normally.
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