Cellular and Molecular Damage by Ganaxolide on Phanerochaete Chrysosporium
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- 英文论文题名：Cellular and Molecular Damage by Ganaxolide on Phanerochaete Chrysosporium
- 论文作者：Hongxia Xiong ; Changbing Liu ; Aijing Li ; Yu Lin ; Wei Huang
- 英文论文作者：Hongxia Xiong ; Changbing Liu ; Aijing Li ; Yu Lin ; Wei Huang ; Tianjin Research Institute for Water Transport Engineering ; Key Laboratory of Environmental Protection in Water transport Engineering ; Ministry of Transport
- 年：2015
- 作者机构：Tianjin Research Institute for Water Transport Engineering,Key Laboratory of Environmental Protection in Water transport Engineering,Ministry of Transport;
- 英文论文关键词：AFLP ; Phanerochaete chrysosporium ; HHCB ; DNA damaging effects
- 会议召开时间：2015-04-01
- 会议录名称：International Journal of Environmental Science and Development Vol.6 No.4
- 语种：英文
- 分类号：X171.5
- 学会代码：CDYA
- 学会名称：成都亚昂教育咨询有限公司
- 页数：5
- 文件大小：1079k
- 原文格式：D
摘要
Galaxolide(1, 3, 4, 6, 7, 8-hexahydro-4, 6, 6, 7, 8,8-hexamethyl-cyclopenta-γ-2-benzopyran, HHCB) is recognized as a novel contaminant in water and has potential adverse impacts on aquatic organisms. The toxic effect of HHCB on Phanerochaete chrysosporium was investigated by exposure of the fungus in nitrogen-limited culture medium to various concentrations of HHCB. The cultivation results showed that both the size and the quantity of the mycelial pellets of P. chrysosporium could be reduced when the concentration of HHCB was higher than 300 mg/L. DNA damage of P.chrysosporium by HHCB was also detected. Comparing with that in the control, the percent polymorphism under different concentrations of HHCB increased, from 21.4% to 42.9%. In addition, the result of UPGMA(un-weighted pair group method of arithmetic means) dendrogram showed that the Simple Matching Coefficient(SM) was decreased with an increase in the concentrations of HHCB. Thus, as an environmental pollutant, HHCB has the toxic effect on P. chrysosporium at both cellular and molecular level.
Galaxolide(1, 3, 4, 6, 7, 8-hexahydro-4, 6, 6, 7, 8,8-hexamethyl-cyclopenta-γ-2-benzopyran, HHCB) is recognized as a novel contaminant in water and has potential adverse impacts on aquatic organisms. The toxic effect of HHCB on Phanerochaete chrysosporium was investigated by exposure of the fungus in nitrogen-limited culture medium to various concentrations of HHCB. The cultivation results showed that both the size and the quantity of the mycelial pellets of P. chrysosporium could be reduced when the concentration of HHCB was higher than 300 mg/L. DNA damage of P.chrysosporium by HHCB was also detected. Comparing with that in the control, the percent polymorphism under different concentrations of HHCB increased, from 21.4% to 42.9%. In addition, the result of UPGMA(un-weighted pair group method of arithmetic means) dendrogram showed that the Simple Matching Coefficient(SM) was decreased with an increase in the concentrations of HHCB. Thus, as an environmental pollutant, HHCB has the toxic effect on P. chrysosporium at both cellular and molecular level.
Galaxolide(1, 3, 4, 6, 7, 8-hexahydro-4, 6, 6, 7, 8,8-hexamethyl-cyclopenta-γ-2-benzopyran, HHCB) is recognized as a novel contaminant in water and has potential adverse impacts on aquatic organisms. The toxic effect of HHCB on Phanerochaete chrysosporium was investigated by exposure of the fungus in nitrogen-limited culture medium to various concentrations of HHCB. The cultivation results showed that both the size and the quantity of the mycelial pellets of P. chrysosporium could be reduced when the concentration of HHCB was higher than 300 mg/L. DNA damage of P.chrysosporium by HHCB was also detected. Comparing with that in the control, the percent polymorphism under different concentrations of HHCB increased, from 21.4% to 42.9%. In addition, the result of UPGMA(un-weighted pair group method of arithmetic means) dendrogram showed that the Simple Matching Coefficient(SM) was decreased with an increase in the concentrations of HHCB. Thus, as an environmental pollutant, HHCB has the toxic effect on P. chrysosporium at both cellular and molecular level.
引文
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[9]M.P.Gooding,T.J.Newton,M.R.Bartsch,and K.C.Hornbuckle,“Toxicity of synthetic musks to early life stages of the freshwater mussel Lampsilis cardium,”Archives Environmental Contamination and Toxicology,vol.51,no.4,pp.549-558,Nov.2006.
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[11]K.L.Sublette,E.V.Ganapathy,and S.Schwartz,“Degradation of munition wastes by Phanerochaete chrysosporium,”App1ied Biochemistry and Biotechnology,vol.34-35,no.1,pp.709-723,Feb.1992.
[12]P.Vos,L.Hogers,M.Bleeker,M.Reijans et al.,“AFLP:A new technique for DNA fingerprinting,”Nucleic Acids Research,vol.23,no.21,pp.4407-4414,Nov.1995.
[13]H.M.Meudt and A.C.Clarke,“Almost forgotten or latest practice?AFLP applications,analyses and advances,”Trends in Plant Science,vol.12,no.3,pp.106-117,Feb.2007.
[14]M.Labra,T.Di Fabio,F.Grassi,S.M.Regondi,M.Bracale,C.Vannini,and E.Agradi,“AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants,”Chemosphere,vol.52,no.7,pp.1183-1188,Aug.2003.
[15]M.Labra,F.De Mattia,M.Bernasconi,D.Bertacchi,F.Grassi,I.Bruni,and S.Citterio,“The combined toxic and genotoxic effects of chromium and volatile organic contaminants to Pseudokirchneriella subcapitata,”Water,Air,and Soil Pollution,vol.213,no.1-4,pp.57-70,Nov.2010.
[16]C.Chen,Q.X.Zhou,S.Liu,and Z.M.Xiu,“Acute toxicity,biochemical and gene expression responses of the earthworm Eisenia fetida exposed to polycyclic musks,”Chemosphere,vol.83,no.8,pp.1147-1154,Feb.2011.
[17]S.A.Walles,“Induction of single-strand breaks in DNA of mice by trichloroethylene and tetrachloroethylene,”Toxicology Letters,vol.31,no.1,pp.31-35,Apr.1986.
[18]M.Labra,M.Bernasconi,F.Grassi,F.De Mattia,S.Sgorbati,R.Airoldi,and S.Citterio,“Toxic and genotoxic effect of potassium dichromate in Pseudokirchneriella subcapitata detected by microscopi and AFLP analysis,”Aquatic Botany,vol.86,no.3,pp.229-235,Apr.2007.
[19]M.Toraason,J.Clark,D.Dankovic,P.Mathias,S.Skaggs,C.Walker,and D.Werren,“Oxidative stress and DNA damage in fischer rats following acute exposure to trichloroethylene or perchloroethylene,”Toxicology,vol.138,no.1,pp.43-53,Oct.1999.
[20]S.S.Wise,A.L.Holmes,and J.P.Wise,“Hexavalent chromium-induced DNA damage and repair mechanisms,”Reviews on Environmental Health,vol.23,no.1,pp.39-57,Jan.-Mar.2008.
[2]G.G.Rimkus,“Polycyclic musk fragrances in the aquatic environment,”Toxicology Letters,vol.111,no.1-2,pp.37-56,Dec.1999.9
[3]X.Y.Zeng,G.Y.Sheng,Y.Xiong,and J.M.Fu,“Determination of polycyclic musks in sewage sludge from Guangdong,China using GCEI-MS,”Chemosphere,vol.60,no.6,pp.8l7-823,Aug.2005.
[4]H.Fromme,T.Otto,and K.Pilz,“Polycylic musk fragrances in different environmental compartments in Berlin(Germany),”Water Research,vol.35,no.1,pp.121-l28,Jan.2001.
[5]A.M.Di Francesco,P.C.Chiu,L.J.Standley,H.E.Allen,and D.T.Salvito,“Dissipation of fragrance materials in sludge-amended soils,”Environmental Science&Technology,vol.38,no.1,pp.194-201,Jan.2000.
[6]M.S.Christian,R.M.Parker,A.M.Hoberman,R.M.Diener,and A.M.Api,“Developmental toxicity studies of four fragrances in rats,”Toxicology Letters,vol.111,no.1-2,pp.169-174,Dec.1999.
[7]A.M.Api and R.A.Ford,“Evaluation of the oral subchronic toxicity of HHCB(1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-gamma-2-benzopyran)in the rat,”Toxicology Letters,vol.111,no.1-2,pp.143-149,Dec.1999.
[8]G.Carlsson and L.Norrgren,“Synthetic musk toxicity to early life stages of zebrafish(Danio rerio),”Archives Environmental Contamination and Toxicology,vol.46,no.1,pp.102-105,Jan.2004.
[9]M.P.Gooding,T.J.Newton,M.R.Bartsch,and K.C.Hornbuckle,“Toxicity of synthetic musks to early life stages of the freshwater mussel Lampsilis cardium,”Archives Environmental Contamination and Toxicology,vol.51,no.4,pp.549-558,Nov.2006.
[10]L.Wollenberger,M.Breitholtz,K.O.Kusk,and B.E.Bengtsson,“Inhibition of larval development of the marine copepod Acartia tonsa by four synthetic musk substances,”The Science of the Total Environment,vol.305,no.1-3,pp.53-64,Apr.2003.
[11]K.L.Sublette,E.V.Ganapathy,and S.Schwartz,“Degradation of munition wastes by Phanerochaete chrysosporium,”App1ied Biochemistry and Biotechnology,vol.34-35,no.1,pp.709-723,Feb.1992.
[12]P.Vos,L.Hogers,M.Bleeker,M.Reijans et al.,“AFLP:A new technique for DNA fingerprinting,”Nucleic Acids Research,vol.23,no.21,pp.4407-4414,Nov.1995.
[13]H.M.Meudt and A.C.Clarke,“Almost forgotten or latest practice?AFLP applications,analyses and advances,”Trends in Plant Science,vol.12,no.3,pp.106-117,Feb.2007.
[14]M.Labra,T.Di Fabio,F.Grassi,S.M.Regondi,M.Bracale,C.Vannini,and E.Agradi,“AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants,”Chemosphere,vol.52,no.7,pp.1183-1188,Aug.2003.
[15]M.Labra,F.De Mattia,M.Bernasconi,D.Bertacchi,F.Grassi,I.Bruni,and S.Citterio,“The combined toxic and genotoxic effects of chromium and volatile organic contaminants to Pseudokirchneriella subcapitata,”Water,Air,and Soil Pollution,vol.213,no.1-4,pp.57-70,Nov.2010.
[16]C.Chen,Q.X.Zhou,S.Liu,and Z.M.Xiu,“Acute toxicity,biochemical and gene expression responses of the earthworm Eisenia fetida exposed to polycyclic musks,”Chemosphere,vol.83,no.8,pp.1147-1154,Feb.2011.
[17]S.A.Walles,“Induction of single-strand breaks in DNA of mice by trichloroethylene and tetrachloroethylene,”Toxicology Letters,vol.31,no.1,pp.31-35,Apr.1986.
[18]M.Labra,M.Bernasconi,F.Grassi,F.De Mattia,S.Sgorbati,R.Airoldi,and S.Citterio,“Toxic and genotoxic effect of potassium dichromate in Pseudokirchneriella subcapitata detected by microscopi and AFLP analysis,”Aquatic Botany,vol.86,no.3,pp.229-235,Apr.2007.
[19]M.Toraason,J.Clark,D.Dankovic,P.Mathias,S.Skaggs,C.Walker,and D.Werren,“Oxidative stress and DNA damage in fischer rats following acute exposure to trichloroethylene or perchloroethylene,”Toxicology,vol.138,no.1,pp.43-53,Oct.1999.
[20]S.S.Wise,A.L.Holmes,and J.P.Wise,“Hexavalent chromium-induced DNA damage and repair mechanisms,”Reviews on Environmental Health,vol.23,no.1,pp.39-57,Jan.-Mar.2008.