Genome-wide SNP discovery and phylogenetic analysis in wheat using genotyping-by-sequencing
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- 英文论文题名：Genome-wide SNP discovery and phylogenetic analysis in wheat using genotyping-by-sequencing
- 论文作者：Jing Wang ; Qiang Gao ; Yao Han ; Haofeng Chen
- 英文论文作者：Jing Wang ; Qiang Gao ; Yao Han ; Haofeng Chen ; Institute of Genetics and Developmental Biology ; Chinese Academy of Sciences
- 年：2016
- 作者机构：Institute of Genetics and Developmental Biology,Chinese Academy of Sciences;
- 英文论文关键词：Genotyping-by-sequencing(GBS) ; wheat ; phylogenetic tree
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S512.1;Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：850k
- 原文格式：D
- 会议级别：全国
摘要
Genotyping-by-sequencing(GBS) is a new powerful method for affordably acquiring dense genome wide marker data for large sample size populations and has been successfully utilized for genetic studies in a variety of species. In this study,1637 wheat accessions from China and other countries were sequenced using GBS method. On average,5,765,333 reads per line were generated,which is equivalent to ~0.02-fold coverage of the wheat genome for each accession. About 15,000 homogeneous SNPs were identified per line. Phylogenetic tree of all accessions inferred from SNPs showed that the collection mainly has six divergent groups,and the accessions with similar geographical origin or hybrid progenies from the same ancestors(such as progenies of Agropyron elongatum×wheat) usually cluster to the same group. These data shed light on the underlying relationship between different wheat accessions and provided phylogenetic reference for future potential breeding utilization.
Genotyping-by-sequencing(GBS) is a new powerful method for affordably acquiring dense genome wide marker data for large sample size populations and has been successfully utilized for genetic studies in a variety of species. In this study,1637 wheat accessions from China and other countries were sequenced using GBS method. On average,5,765,333 reads per line were generated,which is equivalent to ~0.02-fold coverage of the wheat genome for each accession. About 15,000 homogeneous SNPs were identified per line. Phylogenetic tree of all accessions inferred from SNPs showed that the collection mainly has six divergent groups,and the accessions with similar geographical origin or hybrid progenies from the same ancestors(such as progenies of Agropyron elongatum×wheat) usually cluster to the same group. These data shed light on the underlying relationship between different wheat accessions and provided phylogenetic reference for future potential breeding utilization.
Genotyping-by-sequencing(GBS) is a new powerful method for affordably acquiring dense genome wide marker data for large sample size populations and has been successfully utilized for genetic studies in a variety of species. In this study,1637 wheat accessions from China and other countries were sequenced using GBS method. On average,5,765,333 reads per line were generated,which is equivalent to ~0.02-fold coverage of the wheat genome for each accession. About 15,000 homogeneous SNPs were identified per line. Phylogenetic tree of all accessions inferred from SNPs showed that the collection mainly has six divergent groups,and the accessions with similar geographical origin or hybrid progenies from the same ancestors(such as progenies of Agropyron elongatum×wheat) usually cluster to the same group. These data shed light on the underlying relationship between different wheat accessions and provided phylogenetic reference for future potential breeding utilization.
引文