The genetic diversity of Brassica cytoplasmic DNA and beyond
详细信息 查看官网全文
- 英文论文题名：The genetic diversity of Brassica cytoplasmic DNA and beyond
- 论文作者：Jiangwei Qiao ; Xiaojun Zhang ; Mengxian Cai ; Biyun Chen ; Xiaoming Wu
- 英文论文作者：Jiangwei Qiao ; Xiaojun Zhang ; Mengxian Cai ; Biyun Chen ; Xiaoming Wu ; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences
- 年：2016
- 作者机构：Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences;
- 英文论文关键词：Brassica napus ; cpDNA ; genetic diversity ; haplotype ; evolution
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：842k
- 原文格式：D
- 会议级别：全国
摘要
Brassica napus(rapeseed) is a recent allotetraploid plant that is cultivated as the second most important oilseed crop worldwide. Compared with its diploid ancestor species(B. rapa and B. oleracea),B. napus performs much better on many agronomic characters,e.g. colony photosynthetic capacity and economic coefficient. The origin of B. napus and its genetic relationships with its relatives still remain largely unresolved. Here,The cp DNA from a total of 488 worldwide B. napus accessions,139 B. rapa accessions and 49 B. oleracea accessions were populationally re-sequenced using Illumina Solexa sequencing technologies. Their intra-specific cp DNA variants and their allelic frequencies were called genome-widely and further validated by genotyping analyses. The cp DNA of the current worldwide B. napus population comprises more than 400 variants(SNPs and short In Dels) and maintains one predominant haplotype(Bncp1). Sequencing determination of the Bncp1 cp DNA haplotype eliminated its direct inheritance from any of the investigated B. rapa or B. oleracea accessions. The variant-based polymorphism information content(PIC) analysis demonstrated that B. napus has a much lower cp DNA diversity than B. rapa. However,a vast majority of the wild and cultivated B. oleracea appeared to share one same distinct cp DNA haplotype. This finding suggests that the cp DNA of the three Brassica species are well differentiated. Presumably,Bncp1 haplotype may originate from other Brassica relatives. There is also a big possibility that it may result from the interactions between cp DNA mutations and the natural/artificial selection. A series of experiments have been designed and being performed to clarify the evolutionary mechanism,and also to dissect the functional mechanism of the major B. napus cytoplasmic DNA haplotypes. These obtained variation data will provide primary information for the chloroplast/mitochondrion related researches,and germplasm enhancement for B. napus.
Brassica napus(rapeseed) is a recent allotetraploid plant that is cultivated as the second most important oilseed crop worldwide. Compared with its diploid ancestor species(B. rapa and B. oleracea),B. napus performs much better on many agronomic characters,e.g. colony photosynthetic capacity and economic coefficient. The origin of B. napus and its genetic relationships with its relatives still remain largely unresolved. Here,The cp DNA from a total of 488 worldwide B. napus accessions,139 B. rapa accessions and 49 B. oleracea accessions were populationally re-sequenced using Illumina Solexa sequencing technologies. Their intra-specific cp DNA variants and their allelic frequencies were called genome-widely and further validated by genotyping analyses. The cp DNA of the current worldwide B. napus population comprises more than 400 variants(SNPs and short In Dels) and maintains one predominant haplotype(Bncp1). Sequencing determination of the Bncp1 cp DNA haplotype eliminated its direct inheritance from any of the investigated B. rapa or B. oleracea accessions. The variant-based polymorphism information content(PIC) analysis demonstrated that B. napus has a much lower cp DNA diversity than B. rapa. However,a vast majority of the wild and cultivated B. oleracea appeared to share one same distinct cp DNA haplotype. This finding suggests that the cp DNA of the three Brassica species are well differentiated. Presumably,Bncp1 haplotype may originate from other Brassica relatives. There is also a big possibility that it may result from the interactions between cp DNA mutations and the natural/artificial selection. A series of experiments have been designed and being performed to clarify the evolutionary mechanism,and also to dissect the functional mechanism of the major B. napus cytoplasmic DNA haplotypes. These obtained variation data will provide primary information for the chloroplast/mitochondrion related researches,and germplasm enhancement for B. napus.
Brassica napus(rapeseed) is a recent allotetraploid plant that is cultivated as the second most important oilseed crop worldwide. Compared with its diploid ancestor species(B. rapa and B. oleracea),B. napus performs much better on many agronomic characters,e.g. colony photosynthetic capacity and economic coefficient. The origin of B. napus and its genetic relationships with its relatives still remain largely unresolved. Here,The cp DNA from a total of 488 worldwide B. napus accessions,139 B. rapa accessions and 49 B. oleracea accessions were populationally re-sequenced using Illumina Solexa sequencing technologies. Their intra-specific cp DNA variants and their allelic frequencies were called genome-widely and further validated by genotyping analyses. The cp DNA of the current worldwide B. napus population comprises more than 400 variants(SNPs and short In Dels) and maintains one predominant haplotype(Bncp1). Sequencing determination of the Bncp1 cp DNA haplotype eliminated its direct inheritance from any of the investigated B. rapa or B. oleracea accessions. The variant-based polymorphism information content(PIC) analysis demonstrated that B. napus has a much lower cp DNA diversity than B. rapa. However,a vast majority of the wild and cultivated B. oleracea appeared to share one same distinct cp DNA haplotype. This finding suggests that the cp DNA of the three Brassica species are well differentiated. Presumably,Bncp1 haplotype may originate from other Brassica relatives. There is also a big possibility that it may result from the interactions between cp DNA mutations and the natural/artificial selection. A series of experiments have been designed and being performed to clarify the evolutionary mechanism,and also to dissect the functional mechanism of the major B. napus cytoplasmic DNA haplotypes. These obtained variation data will provide primary information for the chloroplast/mitochondrion related researches,and germplasm enhancement for B. napus.
引文