Comparison of the pathogenesis of the highly passaged MCMV Smith strain with that of the low passaged MCMV HaNal isolate in BALB/c mice upon oronasal inoculation
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- 英文论文题名：Comparison of the pathogenesis of the highly passaged MCMV Smith strain with that of the low passaged MCMV HaNal isolate in BALB/c mice upon oronasal inoculation
- 论文作者：Zhang shunchuan ; Xiang jun ; jan Van Doorsselaere ; Hans J.Nauwynck
- 英文论文作者：Zhang shunchuan ; Xiang jun ; jan Van Doorsselaere ; Hans J.Nauwynck ; Laboratory of Virology ; Department of Virology ; Parasitology ; and Immunology ; Faculty of Veterinary Medicine ; Ghent University ; Higher Institute for Nursing and Biotechnology ; VIVES University College
- 年：2016
- 作者机构：Laboratory of Virology,Department of Virology,Parasitology,and Immunology,Faculty of Veterinary Medicine,Ghent University;Higher Institute for Nursing and Biotechnology,VIVES University College;
- 英文论文关键词：MCMV ; Oronasal inoculation ; Pathogenesis
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：英文
- 分类号：S852.65
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：58k
- 原文格式：O
- 会议级别：全国
摘要
Murine cytomegalovirus(MCMV) Smith strain is widely used in mouse models to study HCMV infections.Due to highly serial passages,MCMV Smith has acquired genetic and biological changes.Therefore,a low passaged strain would be more relevant to develop mouse models.Here,the pathogenesis of an infection with MCMV Smith was compared with that of an infection with a low passaged Belgian MCMV isolate HaNal in BALB/c adult mice following oronasal inoculation with either a low(10~4TCID_(50)/mouse) or high(10~6TCID_(50)/mouse) inoculation dose.Both strains were mainly replicating in nasal mucosa and submandibular glands for one to two months.In nasal mucosa,MCMV was detected earlier and longer(1-49dpi) and reached higher titers with the high inoculation dose compared to the low inoculation dose(14-35dpi).In submandibular glands,a similar finding was observed(high dose:7-49dpi;low dose:14-42dpi).In lungs,both strains showed a restricted replication.Only the Smith strain established a low level of productive infection in spleen,liver and kidneys.The infected cells were identified as olfactory neurons and sustentacular cells in olfactory epithelium,macrophages and dendritic cells in NALT,acinar cells in submandibular glands,and macrophages and epithelial cells in lungs for both strains.Antibody analysis demonstrated for both strains that IgG_(2a) was the main detectable antibody subclass.Overall,our results show that significant phenotypic differences exist between the two strains.MCMV HaNal has been shown to be interesting for use in mouse models in order to get better insights for HCMV infections in immunocompetent humans.
Murine cytomegalovirus(MCMV) Smith strain is widely used in mouse models to study HCMV infections.Due to highly serial passages,MCMV Smith has acquired genetic and biological changes.Therefore,a low passaged strain would be more relevant to develop mouse models.Here,the pathogenesis of an infection with MCMV Smith was compared with that of an infection with a low passaged Belgian MCMV isolate HaNal in BALB/c adult mice following oronasal inoculation with either a low(10~4TCID_(50)/mouse) or high(10~6TCID_(50)/mouse) inoculation dose.Both strains were mainly replicating in nasal mucosa and submandibular glands for one to two months.In nasal mucosa,MCMV was detected earlier and longer(1-49dpi) and reached higher titers with the high inoculation dose compared to the low inoculation dose(14-35dpi).In submandibular glands,a similar finding was observed(high dose:7-49dpi;low dose:14-42dpi).In lungs,both strains showed a restricted replication.Only the Smith strain established a low level of productive infection in spleen,liver and kidneys.The infected cells were identified as olfactory neurons and sustentacular cells in olfactory epithelium,macrophages and dendritic cells in NALT,acinar cells in submandibular glands,and macrophages and epithelial cells in lungs for both strains.Antibody analysis demonstrated for both strains that IgG_(2a) was the main detectable antibody subclass.Overall,our results show that significant phenotypic differences exist between the two strains.MCMV HaNal has been shown to be interesting for use in mouse models in order to get better insights for HCMV infections in immunocompetent humans.
Murine cytomegalovirus(MCMV) Smith strain is widely used in mouse models to study HCMV infections.Due to highly serial passages,MCMV Smith has acquired genetic and biological changes.Therefore,a low passaged strain would be more relevant to develop mouse models.Here,the pathogenesis of an infection with MCMV Smith was compared with that of an infection with a low passaged Belgian MCMV isolate HaNal in BALB/c adult mice following oronasal inoculation with either a low(10~4TCID_(50)/mouse) or high(10~6TCID_(50)/mouse) inoculation dose.Both strains were mainly replicating in nasal mucosa and submandibular glands for one to two months.In nasal mucosa,MCMV was detected earlier and longer(1-49dpi) and reached higher titers with the high inoculation dose compared to the low inoculation dose(14-35dpi).In submandibular glands,a similar finding was observed(high dose:7-49dpi;low dose:14-42dpi).In lungs,both strains showed a restricted replication.Only the Smith strain established a low level of productive infection in spleen,liver and kidneys.The infected cells were identified as olfactory neurons and sustentacular cells in olfactory epithelium,macrophages and dendritic cells in NALT,acinar cells in submandibular glands,and macrophages and epithelial cells in lungs for both strains.Antibody analysis demonstrated for both strains that IgG_(2a) was the main detectable antibody subclass.Overall,our results show that significant phenotypic differences exist between the two strains.MCMV HaNal has been shown to be interesting for use in mouse models in order to get better insights for HCMV infections in immunocompetent humans.
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