Changes of anti-oxidation enzymes levels in the blood of MPTP-induced PD monkeys
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- 英文论文题名：Changes of anti-oxidation enzymes levels in the blood of MPTP-induced PD monkeys
- 论文作者：Like Li ; Liangqin Shi ; Heng Liu ; Qihui Luo ; Chao Huang ; Wentao Liu ; Xiaolin Chen ; Wen Zeng ; Zhengli Chen
- 英文论文作者：Like Li ; Liangqin Shi ; Heng Liu ; Qihui Luo ; Chao Huang ; Wentao Liu ; Xiaolin Chen ; Wen Zeng ; Zhengli Chen ; Laboratory of Animal Disease Model ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province ; College of Veterinary Medicine ; Sichuan Agricultural University ; National Experimental Macaque Reproduce Laboratory
- 年：2016
- 作者机构：Laboratory of Animal Disease Model,College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province,College of Veterinary Medicine,Sichuan Agricultural University;National Experimental Macaque Reproduce Laboratory;
- 英文论文关键词：anti-oxidation enzymes ; MPTP ; monkey
- 会议召开时间：2016-08-20
- 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
- 语种：英文
- 分类号：R742.5
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
- 会议地点：中国河南郑州
- 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：66k
- 原文格式：O
- 会议级别：全国
摘要
Parkinson's disease(PD) is an age-related neurodegenerative disease.Although its pathogenic mechanism is not understand,oxidative stress is believed to play a key role in this process.In order to clarify its influence on PD progress,we performed study in eight rhesus monkeys treated with 1l-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) by intramuscular injection to develop a slow evolution of PD.The serum superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH),glutathione peroxidase(GPX),and glutathione-s-transferase(GST) were detected by biochemistry.The whole blood gamma-glutamyl transpeptidase(GGT),gamma-glutamylcysteine synthetase(GCS),glutathione peroxidase 1(GPX-1),and glutathione-s-transferase pi(GST-pi) were measured by RT-PCR.By systemic administration,monkeys gradually developed typical symptoms of PD,while the susceptibility of individual monkeys to the MPTP differed,so the toxin damages of dopaminergic neurons in brain's substantia nigra were dissimilar,and all the monkeys showed distinct clinical behavioral symptoms which lead to the behavioral scores were big difference in the early stage.But all the monkeys subsequently developed the major motor symptoms of PD when receiving an additional treatment with MPTP.Through prolong treated MPTP,monkeys reached a state of stable parkinsonism,and no significant variation was observed subsequently.And the contents of GSH,MDA and the expression of GCS,GST-pi kept increasing,while the activity of GPX,GST,SOD and expression of GPX-1 kept decreasing,no change was observed in GGT expression.This work displayed the antioxidants changes in the blood of MPTP-treated monkeys accompanied with the progresses of PD,and these data suggested a potential identification or diagnosis of PD at different time point through the blood analysis.
Parkinson's disease(PD) is an age-related neurodegenerative disease.Although its pathogenic mechanism is not understand,oxidative stress is believed to play a key role in this process.In order to clarify its influence on PD progress,we performed study in eight rhesus monkeys treated with 1l-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) by intramuscular injection to develop a slow evolution of PD.The serum superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH),glutathione peroxidase(GPX),and glutathione-s-transferase(GST) were detected by biochemistry.The whole blood gamma-glutamyl transpeptidase(GGT),gamma-glutamylcysteine synthetase(GCS),glutathione peroxidase 1(GPX-1),and glutathione-s-transferase pi(GST-pi) were measured by RT-PCR.By systemic administration,monkeys gradually developed typical symptoms of PD,while the susceptibility of individual monkeys to the MPTP differed,so the toxin damages of dopaminergic neurons in brain's substantia nigra were dissimilar,and all the monkeys showed distinct clinical behavioral symptoms which lead to the behavioral scores were big difference in the early stage.But all the monkeys subsequently developed the major motor symptoms of PD when receiving an additional treatment with MPTP.Through prolong treated MPTP,monkeys reached a state of stable parkinsonism,and no significant variation was observed subsequently.And the contents of GSH,MDA and the expression of GCS,GST-pi kept increasing,while the activity of GPX,GST,SOD and expression of GPX-1 kept decreasing,no change was observed in GGT expression.This work displayed the antioxidants changes in the blood of MPTP-treated monkeys accompanied with the progresses of PD,and these data suggested a potential identification or diagnosis of PD at different time point through the blood analysis.
Parkinson's disease(PD) is an age-related neurodegenerative disease.Although its pathogenic mechanism is not understand,oxidative stress is believed to play a key role in this process.In order to clarify its influence on PD progress,we performed study in eight rhesus monkeys treated with 1l-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) by intramuscular injection to develop a slow evolution of PD.The serum superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH),glutathione peroxidase(GPX),and glutathione-s-transferase(GST) were detected by biochemistry.The whole blood gamma-glutamyl transpeptidase(GGT),gamma-glutamylcysteine synthetase(GCS),glutathione peroxidase 1(GPX-1),and glutathione-s-transferase pi(GST-pi) were measured by RT-PCR.By systemic administration,monkeys gradually developed typical symptoms of PD,while the susceptibility of individual monkeys to the MPTP differed,so the toxin damages of dopaminergic neurons in brain's substantia nigra were dissimilar,and all the monkeys showed distinct clinical behavioral symptoms which lead to the behavioral scores were big difference in the early stage.But all the monkeys subsequently developed the major motor symptoms of PD when receiving an additional treatment with MPTP.Through prolong treated MPTP,monkeys reached a state of stable parkinsonism,and no significant variation was observed subsequently.And the contents of GSH,MDA and the expression of GCS,GST-pi kept increasing,while the activity of GPX,GST,SOD and expression of GPX-1 kept decreasing,no change was observed in GGT expression.This work displayed the antioxidants changes in the blood of MPTP-treated monkeys accompanied with the progresses of PD,and these data suggested a potential identification or diagnosis of PD at different time point through the blood analysis.
引文