Genome-wide association study dissects the genetic architecture of oil biosynthesis and plant architecture in rapeseed(Brassica napus L.)
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- 英文论文题名：Genome-wide association study dissects the genetic architecture of oil biosynthesis and plant architecture in rapeseed(Brassica napus L.)
- 论文作者：Jihong Hu ; Biyun Chen ; Hongge Li ; Fugui Zhang ; Guixin Yan ; Guizhen Gao ; Jiangwei Qiao ; Kun Xu ; Hao Li ; Tianyao Zhang ; Lixia Li ; Xin Xiao ; Juan Liu ; Xiaojun Zhang ; Xiaoming Wu
- 英文论文作者：Jihong Hu ; Biyun Chen ; Hongge Li ; Fugui Zhang ; Guixin Yan ; Guizhen Gao ; Jiangwei Qiao ; Kun Xu ; Hao Li ; Tianyao Zhang ; Lixia Li ; Xin Xiao ; Juan Liu ; Xiaojun Zhang ; Xiaoming Wu ; Key Laboratory of Biology and Genetic Improvement of Oil Crops ; Ministry of Agriculture ; Oil Crop Research Institute ; Chinese Academy of Agricultural Science
- 年：2016
- 作者机构：Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Oil Crop Research Institute,Chinese Academy of Agricultural Science;
- 英文论文关键词：Brassica napus ; GWAS ; oil content ; plant architecture ; yield
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S565.4
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：851k
- 原文格式：D
- 会议级别：全国
摘要
Genome-wide association study(GWAS) is a powerful tool for dissect complex agronomic traits in crops species. Rapeseed is one of the most economically important polypoid oil crops in the world. In this study,we re-sequenced 406 diverse rapeseed accessions to construct a haplotype map of the rapeseed genome. Using an Illumina Hi Seq 4000,a total of 55.6 billion paired-end reads of 150 bp in length were generated,with an average coverage depth of more than 10×for each accession. After mapping against the reference sequences of oilseed cultivar ′Darmor-bzh′,28,006,956 single nucleotide polymorphisms(SNPs) were identified. Using these SNPs,genome-wide association studies were performed for mapping traits. We investigate 36 agronomic traits in four environments and examined the major loci of genes related to oil content,fatty acid biosynthesis,seed weight,plant height and primary branch. Many of the candidate genes for oil content encode enzymes involved in oil metabolism,including two significant associations on A8 and C3 of Brassica napus which were close to the key gene Bna. FAE1 for erucic acid content. Some other QTLs were also identified for plant architecture(plant height and primary branch). Our results provide insights into the genetic basis of oil biosynthesis in oilseed and may facilitate marker-based breeding for oil production and yield.
Genome-wide association study(GWAS) is a powerful tool for dissect complex agronomic traits in crops species. Rapeseed is one of the most economically important polypoid oil crops in the world. In this study,we re-sequenced 406 diverse rapeseed accessions to construct a haplotype map of the rapeseed genome. Using an Illumina Hi Seq 4000,a total of 55.6 billion paired-end reads of 150 bp in length were generated,with an average coverage depth of more than 10×for each accession. After mapping against the reference sequences of oilseed cultivar ′Darmor-bzh′,28,006,956 single nucleotide polymorphisms(SNPs) were identified. Using these SNPs,genome-wide association studies were performed for mapping traits. We investigate 36 agronomic traits in four environments and examined the major loci of genes related to oil content,fatty acid biosynthesis,seed weight,plant height and primary branch. Many of the candidate genes for oil content encode enzymes involved in oil metabolism,including two significant associations on A8 and C3 of Brassica napus which were close to the key gene Bna. FAE1 for erucic acid content. Some other QTLs were also identified for plant architecture(plant height and primary branch). Our results provide insights into the genetic basis of oil biosynthesis in oilseed and may facilitate marker-based breeding for oil production and yield.
Genome-wide association study(GWAS) is a powerful tool for dissect complex agronomic traits in crops species. Rapeseed is one of the most economically important polypoid oil crops in the world. In this study,we re-sequenced 406 diverse rapeseed accessions to construct a haplotype map of the rapeseed genome. Using an Illumina Hi Seq 4000,a total of 55.6 billion paired-end reads of 150 bp in length were generated,with an average coverage depth of more than 10×for each accession. After mapping against the reference sequences of oilseed cultivar ′Darmor-bzh′,28,006,956 single nucleotide polymorphisms(SNPs) were identified. Using these SNPs,genome-wide association studies were performed for mapping traits. We investigate 36 agronomic traits in four environments and examined the major loci of genes related to oil content,fatty acid biosynthesis,seed weight,plant height and primary branch. Many of the candidate genes for oil content encode enzymes involved in oil metabolism,including two significant associations on A8 and C3 of Brassica napus which were close to the key gene Bna. FAE1 for erucic acid content. Some other QTLs were also identified for plant architecture(plant height and primary branch). Our results provide insights into the genetic basis of oil biosynthesis in oilseed and may facilitate marker-based breeding for oil production and yield.
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