肠炎沙门氏菌、金黄色葡萄球菌、大肠杆菌O157:H7和单增李斯特菌共增培养基SSEL研究
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- 英文论文题名：SSEL,a selective enrichment broth for simultaneous growth of Salmonella Enterica,Staphylococcus aureus,Escherichia coli O157:H7 and Listeria monocytogenes
- 论文作者：瞿洋 ; 沈源源 ; 周昌艳 ; 索玉娟
- 英文论文作者：Qu Yang ; Shen Yuanyuan ; Zhou Changyan ; Suo Yujuan ; Insitute of Agro-food Standard and Testing Technology ; Shanghai Academy of Agriculture Science ; School of Medical Instrument and Food Engineering ; University of Shanghai for Science and Technology
- 年：2016
- 作者机构：上海市农业科学院农产品质量标准与检测技术研究所;上海理工大学医疗器械与食品学院;
- 论文关键词：共增培养基 ; 沙门氏菌 ; 金黄色葡萄球菌 ; 大肠杆菌O157 ; H7 ; 单增李斯特菌
- 英文论文关键词：multi-pathogen enrichment broth ; salmonella enterica ; staphylococcus aureus ; escherichia coli O157 ; H7 ; listeria monocytogenes
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：104k
- 原文格式：O
- 会议级别：全国
摘要
本研究为实现肠炎沙门氏菌、金黄色葡萄球菌、大肠杆菌O157:H7和单增李斯菌的同步快速检测,设计并制备了一种适于上述4种菌的共增菌选择性培养基(SSEL)。首先,以TSB-YE为基础培养基,对抑菌剂进行筛选,经单因素和正交实验确定最佳的成分及配比;其次,分析SSEL的增菌能力及增菌特异性;最后,通过人工污染试验,评价SSEL对低温贮藏下的肉制品及生菜中目标菌的增菌修复能力。结果显示,SSEL的单增菌效果与国标中目标菌各自的选择性培养基一致,不同接种比例的目标菌经过24 h富集后均可达到10~5 CFU/mL以上;此外,SSEL对非目标菌有一定的抑制效果,并能有效修复低温肉制品及生菜中冷损伤的目标致病菌。可见,SSEL可用于样品尤其是冷藏样品中肠炎沙门氏菌、金黄色葡萄球菌、大肠杆菌O157:H7和单增李斯特检测的共增菌培养,并有望与多重PCR联用加快检测时间,节约检测成本与样品的消耗量。
In order to simultaneously detect Salmonella Enterica,Staphylococcus aureus,Escherichia coli 0157:H7 and Listeria monocytogenes on a single-assay platform,a multi-pathogen selective enrichment broth(SSEL) was developed for the four target bacterial.Firstly,several candidate bacteriostatic agents were added to Tryptic Soy Broth With Yeast Extract(TSB-YE) to determine their best composition and proportion by single factor and orthogonal test.Secondly,the enrichment ability and specificity of SSEL were analyzed.Finally,the repair ability of SSEL for target bacteria in the meat and lettuce under the low temperature storage were assessed through the artificial contamination test.As a result,SSEL show the same effects of enrichment for the four target bacterial with the selective enrichment broths recommended by National Standards,and the four target pathogens could grow to 10 CFU/mL after being inoculated with different concentration in SSEL and cultured together for 24 h;In addition,SSEL broth had certain inhibition effect for the non-target organisms,and could efficiently recover target pathogens suffered cold stress.It was shown that SSEL could be applied for the simultaneous pre-enrichment of S.Enterica,S.aureus,E.coli 0157:H7 and L.monocytogenes in food samples especially in low-temperature meat products and lettuce,and be expected for accelerating the test time and saving the testing cost and the sample consumption combined with multiple PCR.
In order to simultaneously detect Salmonella Enterica,Staphylococcus aureus,Escherichia coli 0157:H7 and Listeria monocytogenes on a single-assay platform,a multi-pathogen selective enrichment broth(SSEL) was developed for the four target bacterial.Firstly,several candidate bacteriostatic agents were added to Tryptic Soy Broth With Yeast Extract(TSB-YE) to determine their best composition and proportion by single factor and orthogonal test.Secondly,the enrichment ability and specificity of SSEL were analyzed.Finally,the repair ability of SSEL for target bacteria in the meat and lettuce under the low temperature storage were assessed through the artificial contamination test.As a result,SSEL show the same effects of enrichment for the four target bacterial with the selective enrichment broths recommended by National Standards,and the four target pathogens could grow to 10 CFU/mL after being inoculated with different concentration in SSEL and cultured together for 24 h;In addition,SSEL broth had certain inhibition effect for the non-target organisms,and could efficiently recover target pathogens suffered cold stress.It was shown that SSEL could be applied for the simultaneous pre-enrichment of S.Enterica,S.aureus,E.coli 0157:H7 and L.monocytogenes in food samples especially in low-temperature meat products and lettuce,and be expected for accelerating the test time and saving the testing cost and the sample consumption combined with multiple PCR.
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