The Histological Characteristic,Age-related Thickness Change of Skin,and Expression of the Heat Shock Protein-27 in the Skin during Hair Cycle in Yak(Bos grunniens)
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- 英文论文题名：The Histological Characteristic,Age-related Thickness Change of Skin,and Expression of the Heat Shock Protein-27 in the Skin during Hair Cycle in Yak(Bos grunniens)
- 论文作者：Xue Yang ; Yan Cui ; Jing Yue ; Honghong He ; Chuan Yu ; Xiandong Ren ; Yun Meng
- 英文论文作者：Xue Yang ; Yan Cui ; Jing Yue ; Honghong He ; Chuan Yu ; Xiandong Ren ; Yun Meng ; Laboratory of Animal Anatomy & Tissue Embryology ; Department of Basic Veterinary Medicine ; College of Veterinary Medicine ; Gansu Agricultural University ; Gansu Province Livestock Embryo Engineering Research Center ; Department of Clinical Veterinary Medicine ; College of Veterinary Medicine ; Gansu Agricultural University
- 年：2016
- 作者机构：Laboratory of Animal Anatomy & Tissue Embryology,Department of Basic Veterinary Medicine,College of Veterinary Medicine,Gansu Agricultural University;Gansu Province Livestock Embryo Engineering Research Center,Department of Clinical Veterinary Medicine,College of Veterinary Medicine,Gansu Agricultural University;
- 英文论文关键词：Yak ; Skin ; Histological characteristic ; Thickness ; Hair follicle ; HSP27
- 会议召开时间：2016-08-20
- 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
- 语种：英文
- 分类号：S823.85
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
- 会议地点：中国河南郑州
- 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
- 学会名称：中国畜牧兽医学会
- 页数：2
- 文件大小：134k
- 原文格式：O
- 会议级别：全国
摘要
This experiment was conducted to study the histological characteristics,age-related thickness changes,and expression of HSP27 in the skin of yak.Methods:A total of 20 yaks(10males and 10 females) was used.Different regions of the normal skin of three different ages(newborn,half-year-old and adult) of yaks were harvested for histological study and thickness measurement.Biopsy samples were taken from the scapula regions of the skin of the same five approximately 1-year-old yaks during the hair cycle(telogen,anagen and catagen).RT-PCR,western blot and immunohistochemistry methods using th mRNA and protein levels were used to detect the expression of HSP27.The IPP6.0 software was used to analyze the immunohistochemistry and measure the thickness of the skin.Results:The general histological structure of hairy yak skin was similar to other domestic mammals.The unique features included prominent cutaneous vascular plexuses,underdeveloped sweat glands,a large number of nasolabial glands in the nasolabial plate,and hair follicle groups composed of one primary follicle and several secondary follicles.The skin,epidermis and dermis thickness did vary significantly between different body regions and different ages.The thickness of the skin,epidermis and dermis increased from newborn to adult in yaks.Yak skin thickness decreased dorsally to ventrally on the trunk.The skin on the lateral surface was thicker than the skin on the medial surface on the limbs.RT-PCR,western blot and immunohistochemistry showed the same expression patterns of HSP27 mRNA and protein in the skin during the hair cycle.HSP27 was expressed in the epidermis and the outer root sheath of the secondary follicle in the skin of yak.The expression of HSP27 mRNA and protein in the anagen stage was the highest,followed by the catagen stage,and the expression in the telogen stage was the lowest.Meaning:In this study,we examined and fully described the histology of normal skin in 45 yak and measured the skin thickness of different ages and different regions in yak.These data may be useful to better understand and appreciate the adaptability features of yak skin.Our investigation reports the expression patterns of HSP27 in yak skin for the first time.The different expression of HSP27 during the hair cycle suggests its possible role in yak hair follicle biology.
This experiment was conducted to study the histological characteristics,age-related thickness changes,and expression of HSP27 in the skin of yak.Methods:A total of 20 yaks(10males and 10 females) was used.Different regions of the normal skin of three different ages(newborn,half-year-old and adult) of yaks were harvested for histological study and thickness measurement.Biopsy samples were taken from the scapula regions of the skin of the same five approximately 1-year-old yaks during the hair cycle(telogen,anagen and catagen).RT-PCR,western blot and immunohistochemistry methods using th mRNA and protein levels were used to detect the expression of HSP27.The IPP6.0 software was used to analyze the immunohistochemistry and measure the thickness of the skin.Results:The general histological structure of hairy yak skin was similar to other domestic mammals.The unique features included prominent cutaneous vascular plexuses,underdeveloped sweat glands,a large number of nasolabial glands in the nasolabial plate,and hair follicle groups composed of one primary follicle and several secondary follicles.The skin,epidermis and dermis thickness did vary significantly between different body regions and different ages.The thickness of the skin,epidermis and dermis increased from newborn to adult in yaks.Yak skin thickness decreased dorsally to ventrally on the trunk.The skin on the lateral surface was thicker than the skin on the medial surface on the limbs.RT-PCR,western blot and immunohistochemistry showed the same expression patterns of HSP27 mRNA and protein in the skin during the hair cycle.HSP27 was expressed in the epidermis and the outer root sheath of the secondary follicle in the skin of yak.The expression of HSP27 mRNA and protein in the anagen stage was the highest,followed by the catagen stage,and the expression in the telogen stage was the lowest.Meaning:In this study,we examined and fully described the histology of normal skin in 45 yak and measured the skin thickness of different ages and different regions in yak.These data may be useful to better understand and appreciate the adaptability features of yak skin.Our investigation reports the expression patterns of HSP27 in yak skin for the first time.The different expression of HSP27 during the hair cycle suggests its possible role in yak hair follicle biology.
This experiment was conducted to study the histological characteristics,age-related thickness changes,and expression of HSP27 in the skin of yak.Methods:A total of 20 yaks(10males and 10 females) was used.Different regions of the normal skin of three different ages(newborn,half-year-old and adult) of yaks were harvested for histological study and thickness measurement.Biopsy samples were taken from the scapula regions of the skin of the same five approximately 1-year-old yaks during the hair cycle(telogen,anagen and catagen).RT-PCR,western blot and immunohistochemistry methods using th mRNA and protein levels were used to detect the expression of HSP27.The IPP6.0 software was used to analyze the immunohistochemistry and measure the thickness of the skin.Results:The general histological structure of hairy yak skin was similar to other domestic mammals.The unique features included prominent cutaneous vascular plexuses,underdeveloped sweat glands,a large number of nasolabial glands in the nasolabial plate,and hair follicle groups composed of one primary follicle and several secondary follicles.The skin,epidermis and dermis thickness did vary significantly between different body regions and different ages.The thickness of the skin,epidermis and dermis increased from newborn to adult in yaks.Yak skin thickness decreased dorsally to ventrally on the trunk.The skin on the lateral surface was thicker than the skin on the medial surface on the limbs.RT-PCR,western blot and immunohistochemistry showed the same expression patterns of HSP27 mRNA and protein in the skin during the hair cycle.HSP27 was expressed in the epidermis and the outer root sheath of the secondary follicle in the skin of yak.The expression of HSP27 mRNA and protein in the anagen stage was the highest,followed by the catagen stage,and the expression in the telogen stage was the lowest.Meaning:In this study,we examined and fully described the histology of normal skin in 45 yak and measured the skin thickness of different ages and different regions in yak.These data may be useful to better understand and appreciate the adaptability features of yak skin.Our investigation reports the expression patterns of HSP27 in yak skin for the first time.The different expression of HSP27 during the hair cycle suggests its possible role in yak hair follicle biology.
引文