Methyl tert-butyl ether could induce autophagy in HT-22 cells
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- 英文论文题名：Methyl tert-butyl ether could induce autophagy in HT-22 cells
- 论文作者：Junxiang Ma ; Li Chen ; Dongmei Song ; Yuanyuan Zhang ; Piye Niu
- 英文论文作者：Junxiang Ma ; Li Chen ; Dongmei Song ; Yuanyuan Zhang ; Piye Niu ; Department of Occupational Health and Environmental Health ; School of Public Health ; Capital Medical University ; Beijing Key Laboratory of Environmental Toxicology ; School of Public Health ; Capital Medical University
- 年：2016
- 作者机构：Department of Occupational Health and Environmental Health,School of Public Health,Capital Medical University;Beijing Key Laboratory of Environmental Toxicology,School of Public Health,Capital Medical University;
- 英文论文关键词：Methyl tert-butyl ether(MTBE) ; Autophagy ; HT-cells
- 会议召开时间：2016-12-03
- 会议录名称：2016中国毒理学会神经毒理专业委员会学术年会论文集
- 语种：英文
- 分类号：R114
- 学会代码：ZGDV
- 会议名称：2016中国毒理学会神经毒理专业委员会学术年会
- 会议地点：中国北京
- 主办单位：中国毒理学会神经毒理专业委员会
- 学会名称：中国毒理学会
- 页数：1
- 文件大小：63k
- 原文格式：O
- 会议级别：全国
摘要
Objective:Autophagy induced by methyl tert-butyl ether(MTBE) in HT-22 cells were investigated to elucidate the mechanism of MTBE toxicity.Method:In this study,HT-22 mouse hippocampal neurons cells were used.To obverse the toxicity of MTBE in HT-22 cells,we exposed cells to different concentrations of MTBE(0,0.125,0.25,0.5,1.0,2.0,2.5 and 5.0 mM) for 6 h and the survival rate of cells were detected by MTT.Then HT-22 cells were treated with MTBE(0,0.125,0.25,0.5 and1.0 mM) for 1,2,4 and 6 h,respectively,and the expressions of autophagy marker proteins,LC3-I,LC3-II and p62 were detected by WB.The morphology of autophagosome was observed by transmission electron microscopy(TEM).Results:1) MTT results showed that the survival rate of cells were reduced dose-dependently with increase of MTBE concentration(P < 0.05),and the cells survival rate were above85%only in 0.125,0.25,0.5 and 1.0 mM,which were chose as the following treatment concentration.2)Compared with the control,the expression of autophagy marker proteins LC3-I,LC3-II and p62 showed significant time-dose effect(P < 0.05) in cells exposed to different doses of MTBE(0.125,0.25,0.5 and1.0 mM) for 1,2,4 and 6 h,respectively.In general,the ratio of LC3-II/LC3-I was the higher(P < 0.05)and the expression of p62 was the lower(P < 0.05),in cells were treated with MTBE for 2 h,especially in dose 0.25 mM(P < 0.05).The cells autophagy results indicated that the phenomenon of cells autophagy were more obvious in 0.25 mM MTBE-treated for 2 h,and was inhibited with increase of MTBE-treated dose and MTBE-treated time.Obvious double membrane structure,the typical autophagic morphology,was observed in cells of MTBE-treated for 2 h.Conclusions:MTBE in low dose can induce autophagy in HT-22 cells,which may play a protective effect on HT-22 cells.But the autophagy may be inhibited with the increase of MTBE-treated dose and MTBE-treated time,which may play a harmful effect on HT-22 cells.
Objective:Autophagy induced by methyl tert-butyl ether(MTBE) in HT-22 cells were investigated to elucidate the mechanism of MTBE toxicity.Method:In this study,HT-22 mouse hippocampal neurons cells were used.To obverse the toxicity of MTBE in HT-22 cells,we exposed cells to different concentrations of MTBE(0,0.125,0.25,0.5,1.0,2.0,2.5 and 5.0 mM) for 6 h and the survival rate of cells were detected by MTT.Then HT-22 cells were treated with MTBE(0,0.125,0.25,0.5 and1.0 mM) for 1,2,4 and 6 h,respectively,and the expressions of autophagy marker proteins,LC3-I,LC3-II and p62 were detected by WB.The morphology of autophagosome was observed by transmission electron microscopy(TEM).Results:1) MTT results showed that the survival rate of cells were reduced dose-dependently with increase of MTBE concentration(P < 0.05),and the cells survival rate were above85%only in 0.125,0.25,0.5 and 1.0 mM,which were chose as the following treatment concentration.2)Compared with the control,the expression of autophagy marker proteins LC3-I,LC3-II and p62 showed significant time-dose effect(P < 0.05) in cells exposed to different doses of MTBE(0.125,0.25,0.5 and1.0 mM) for 1,2,4 and 6 h,respectively.In general,the ratio of LC3-II/LC3-I was the higher(P < 0.05)and the expression of p62 was the lower(P < 0.05),in cells were treated with MTBE for 2 h,especially in dose 0.25 mM(P < 0.05).The cells autophagy results indicated that the phenomenon of cells autophagy were more obvious in 0.25 mM MTBE-treated for 2 h,and was inhibited with increase of MTBE-treated dose and MTBE-treated time.Obvious double membrane structure,the typical autophagic morphology,was observed in cells of MTBE-treated for 2 h.Conclusions:MTBE in low dose can induce autophagy in HT-22 cells,which may play a protective effect on HT-22 cells.But the autophagy may be inhibited with the increase of MTBE-treated dose and MTBE-treated time,which may play a harmful effect on HT-22 cells.
Objective:Autophagy induced by methyl tert-butyl ether(MTBE) in HT-22 cells were investigated to elucidate the mechanism of MTBE toxicity.Method:In this study,HT-22 mouse hippocampal neurons cells were used.To obverse the toxicity of MTBE in HT-22 cells,we exposed cells to different concentrations of MTBE(0,0.125,0.25,0.5,1.0,2.0,2.5 and 5.0 mM) for 6 h and the survival rate of cells were detected by MTT.Then HT-22 cells were treated with MTBE(0,0.125,0.25,0.5 and1.0 mM) for 1,2,4 and 6 h,respectively,and the expressions of autophagy marker proteins,LC3-I,LC3-II and p62 were detected by WB.The morphology of autophagosome was observed by transmission electron microscopy(TEM).Results:1) MTT results showed that the survival rate of cells were reduced dose-dependently with increase of MTBE concentration(P < 0.05),and the cells survival rate were above85%only in 0.125,0.25,0.5 and 1.0 mM,which were chose as the following treatment concentration.2)Compared with the control,the expression of autophagy marker proteins LC3-I,LC3-II and p62 showed significant time-dose effect(P < 0.05) in cells exposed to different doses of MTBE(0.125,0.25,0.5 and1.0 mM) for 1,2,4 and 6 h,respectively.In general,the ratio of LC3-II/LC3-I was the higher(P < 0.05)and the expression of p62 was the lower(P < 0.05),in cells were treated with MTBE for 2 h,especially in dose 0.25 mM(P < 0.05).The cells autophagy results indicated that the phenomenon of cells autophagy were more obvious in 0.25 mM MTBE-treated for 2 h,and was inhibited with increase of MTBE-treated dose and MTBE-treated time.Obvious double membrane structure,the typical autophagic morphology,was observed in cells of MTBE-treated for 2 h.Conclusions:MTBE in low dose can induce autophagy in HT-22 cells,which may play a protective effect on HT-22 cells.But the autophagy may be inhibited with the increase of MTBE-treated dose and MTBE-treated time,which may play a harmful effect on HT-22 cells.
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