Probing the nuclear import signal and nuclear transport molecular determinants of PRV ICP22
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- 英文论文题名：Probing the nuclear import signal and nuclear transport molecular determinants of PRV ICP22
- 论文作者：Cai mingsheng ; Jiang si ; Zeng zhancheng ; Li xiaowei ; Mo chuncong ; Yang yanjia ; Chen chunke ; Xie peiping ; Bian yun ; Wang jinglin ; Huang jinlu ; Chen daixiong ; Peng tao ; Li meili
- 英文论文作者：Cai mingsheng ; Jiang si ; Zeng zhancheng ; Li xiaowei ; Mo chuncong ; Yang yanjia ; Chen chunke ; Xie peiping ; Bian yun ; Wang jinglin ; Huang jinlu ; Chen daixiong ; Peng tao ; Li meili ; Department of Pathogenic Biology and Immunology ; School of Basic Science ; Guangzhou Medical University ; Guangzhou Hoffmann Institute of Immunology ; School of Basic Science ; Guangzhou Medical University ; Guangdong Haid Group Co. ; Ltd.
- 年：2016
- 作者机构：Department of Pathogenic Biology and Immunology,School of Basic Science,Guangzhou Medical University;Guangzhou Hoffmann Institute of Immunology,School of Basic Science,Guangzhou Medical University;Guangdong Haid Group Co.,Ltd.;
- 英文论文关键词：PRV ICP22 ; nuclear transport ; nuclear localization signal(NLS) ; importin ; Ran-GTP
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：英文
- 分类号：S852.65
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：11
- 文件大小：4308k
- 原文格式：O
- 会议级别：全国
摘要
(Background):Herpes simplex virus 1(HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replication.Pseudorabies virus(PRV) ICP22(P-ICP22) is a homologue of HSV-1 ICP22 and is reported to be able to selectively modify the transcription of different kinetic classes of PRV genes,however,the subcellular localization,localization signal and molecular determinants for its transportto execute this function is less well understood.Findings:In this study,by utilizing live cells fluorescent microscopy,P-ICP22 fused to enhanced yellow fluorescent protein(EYFP) gene was transient expressed in live cells and shown to exhibit a predominantly nucleus localization in the absence of other viral proteins.By transfection of a series of P-ICP22 deletion mutants fused to EYFP,a bona fide nuclear localization signal(NLS) and its key amino acids(aa) of P-ICP22 was,for the first time,determined and mapped to aa 41 to 60(PASTPTPPKRGRYVVEHPEY) and aa 49 to50(KR),respectively.Besides,the P-ICP22 was demonstrated to be targeted to thenucleusvia Ran-,importin α1-,and α7-mediated pathway.Conclusions:Our findings reported herein disclose the NLS and molecular mechanism for nuclear transport of P-ICP22,these results will uncover new avenues for depicting the biological roles of P-ICP22 during PRV infection.
(Background):Herpes simplex virus 1(HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replication.Pseudorabies virus(PRV) ICP22(P-ICP22) is a homologue of HSV-1 ICP22 and is reported to be able to selectively modify the transcription of different kinetic classes of PRV genes,however,the subcellular localization,localization signal and molecular determinants for its transportto execute this function is less well understood.Findings:In this study,by utilizing live cells fluorescent microscopy,P-ICP22 fused to enhanced yellow fluorescent protein(EYFP) gene was transient expressed in live cells and shown to exhibit a predominantly nucleus localization in the absence of other viral proteins.By transfection of a series of P-ICP22 deletion mutants fused to EYFP,a bona fide nuclear localization signal(NLS) and its key amino acids(aa) of P-ICP22 was,for the first time,determined and mapped to aa 41 to 60(PASTPTPPKRGRYVVEHPEY) and aa 49 to50(KR),respectively.Besides,the P-ICP22 was demonstrated to be targeted to thenucleusvia Ran-,importin α1-,and α7-mediated pathway.Conclusions:Our findings reported herein disclose the NLS and molecular mechanism for nuclear transport of P-ICP22,these results will uncover new avenues for depicting the biological roles of P-ICP22 during PRV infection.
(Background):Herpes simplex virus 1(HSV-1) ICP22 is a multifunctional protein and important for HSV-1 replication.Pseudorabies virus(PRV) ICP22(P-ICP22) is a homologue of HSV-1 ICP22 and is reported to be able to selectively modify the transcription of different kinetic classes of PRV genes,however,the subcellular localization,localization signal and molecular determinants for its transportto execute this function is less well understood.Findings:In this study,by utilizing live cells fluorescent microscopy,P-ICP22 fused to enhanced yellow fluorescent protein(EYFP) gene was transient expressed in live cells and shown to exhibit a predominantly nucleus localization in the absence of other viral proteins.By transfection of a series of P-ICP22 deletion mutants fused to EYFP,a bona fide nuclear localization signal(NLS) and its key amino acids(aa) of P-ICP22 was,for the first time,determined and mapped to aa 41 to 60(PASTPTPPKRGRYVVEHPEY) and aa 49 to50(KR),respectively.Besides,the P-ICP22 was demonstrated to be targeted to thenucleusvia Ran-,importin α1-,and α7-mediated pathway.Conclusions:Our findings reported herein disclose the NLS and molecular mechanism for nuclear transport of P-ICP22,these results will uncover new avenues for depicting the biological roles of P-ICP22 during PRV infection.
引文