Effect of Saposhnikovia divaricata(Fangfeng) Ethylacetate Extract on the Production of IL-1β, IL-6 and NLRP3 in LPS-induced U937 Cells
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- 英文论文题名：Effect of Saposhnikovia divaricata(Fangfeng) Ethylacetate Extract on the Production of IL-1β, IL-6 and NLRP3 in LPS-induced U937 Cells
- 论文作者：QIU Xue-mei ; FAN Dan-ping ; LIU Xue ; Nikles Stefanie ; Bauer Rudolf ; Lu Ai-ping ; HE Xiao-juan
- 英文论文作者：QIU Xue-mei ; FAN Dan-ping ; LIU Xue ; Nikles Stefanie ; Bauer Rudolf ; Lu Ai-ping ; HE Xiao-juan ; Institute of Basic Research in Clinical Medicine ; China Academy of Chinese Medical Sciences ; School of Life Science and Engineering ; Southwest Jiaotong University ; Institute of Pharmaceutical Sciences ; Department of Pharmacognosy ; University of Graz ; School of Chinese Medicine ; Hong Kong Baptist University
- 年：2016
- 作者机构：Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences;School of Life Science and Engineering,Southwest Jiaotong University;Institute of Pharmaceutical Sciences,Department of Pharmacognosy,University of Graz;School of Chinese Medicine,Hong Kong Baptist University;
- 英文论文关键词：Saposhnikovia divaricata ; ethylacetate ; NLRP3 ; cytokine ; U937
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R285
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1353k
- 原文格式：D
- 会议级别：全国
摘要
Background: Saposhnikovia divaricate(Fangfeng), a traditional Chinese medicine, has been commonly used for some chronic inflammatory diseases such as arthritis. However, the underlying functional mechanism is still unknown. Objective: The aim of this study is to investigate the effect of Saposhnikovia divaricate ethylacetate extract(SDE) on the production of IL-1β, IL-6 and NLRP3 in LPS-induced U937 Cells. Methods: Saposhnikovia divaricate has been extracted with ethylacetate(Et OAc). U937 cells were pre-treated with different concentrations(0, 3.125, 6.25 12.5, 25, 50, 100, 200 μg/ml) of SDE after being induced with PMA(10 ng/ml) for 48 h into macrophages. The cell viability was determined by CCK-8 assay. Furthermore, U937 cells induced by PMA were stimulated with LPS(1 μg/ml) for 48 h with or without the indicated concentration(25 μg/ml) of SDE for 2 h, followed by ATP(5 m M) treatment for 45 min. The mRNA levels of IL-1β, IL-6 and NLRP3 in the cells and the protein levels of these factors in the supernatant were assessed by real-time PCR and ELISA, respectively.Results: Our results showed that SDE didn't exhibit obvious cytotoxicity at the concentration of 3.125, 6.25 12.5 and 25 μg/ml. Subsequently, 25 μg/ml SDE was used for the further experiments. We found that IL-1β, IL-6 and NLRP3 mRNA levels in cells and protein levels in supernatant were all significantly increased in LPS combined with ATP-stimulated U937 cells compared to the control group. SDE could significantly downregulate the mRNA and protein levels of IL-1β, IL-6 and NLRP3 in SDE-treated groups compared to the LPS combined with ATP-stimulated group. Conclusion: These results showed that SDE could inhibit the production of IL-1β, IL-6 and NLRP3 in LPS combined with ATP-stimulated U937 cells.
Background: Saposhnikovia divaricate(Fangfeng), a traditional Chinese medicine, has been commonly used for some chronic inflammatory diseases such as arthritis. However, the underlying functional mechanism is still unknown. Objective: The aim of this study is to investigate the effect of Saposhnikovia divaricate ethylacetate extract(SDE) on the production of IL-1β, IL-6 and NLRP3 in LPS-induced U937 Cells. Methods: Saposhnikovia divaricate has been extracted with ethylacetate(Et OAc). U937 cells were pre-treated with different concentrations(0, 3.125, 6.25 12.5, 25, 50, 100, 200 μg/ml) of SDE after being induced with PMA(10 ng/ml) for 48 h into macrophages. The cell viability was determined by CCK-8 assay. Furthermore, U937 cells induced by PMA were stimulated with LPS(1 μg/ml) for 48 h with or without the indicated concentration(25 μg/ml) of SDE for 2 h, followed by ATP(5 m M) treatment for 45 min. The mRNA levels of IL-1β, IL-6 and NLRP3 in the cells and the protein levels of these factors in the supernatant were assessed by real-time PCR and ELISA, respectively.Results: Our results showed that SDE didn't exhibit obvious cytotoxicity at the concentration of 3.125, 6.25 12.5 and 25 μg/ml. Subsequently, 25 μg/ml SDE was used for the further experiments. We found that IL-1β, IL-6 and NLRP3 mRNA levels in cells and protein levels in supernatant were all significantly increased in LPS combined with ATP-stimulated U937 cells compared to the control group. SDE could significantly downregulate the mRNA and protein levels of IL-1β, IL-6 and NLRP3 in SDE-treated groups compared to the LPS combined with ATP-stimulated group. Conclusion: These results showed that SDE could inhibit the production of IL-1β, IL-6 and NLRP3 in LPS combined with ATP-stimulated U937 cells.
Background: Saposhnikovia divaricate(Fangfeng), a traditional Chinese medicine, has been commonly used for some chronic inflammatory diseases such as arthritis. However, the underlying functional mechanism is still unknown. Objective: The aim of this study is to investigate the effect of Saposhnikovia divaricate ethylacetate extract(SDE) on the production of IL-1β, IL-6 and NLRP3 in LPS-induced U937 Cells. Methods: Saposhnikovia divaricate has been extracted with ethylacetate(Et OAc). U937 cells were pre-treated with different concentrations(0, 3.125, 6.25 12.5, 25, 50, 100, 200 μg/ml) of SDE after being induced with PMA(10 ng/ml) for 48 h into macrophages. The cell viability was determined by CCK-8 assay. Furthermore, U937 cells induced by PMA were stimulated with LPS(1 μg/ml) for 48 h with or without the indicated concentration(25 μg/ml) of SDE for 2 h, followed by ATP(5 m M) treatment for 45 min. The mRNA levels of IL-1β, IL-6 and NLRP3 in the cells and the protein levels of these factors in the supernatant were assessed by real-time PCR and ELISA, respectively.Results: Our results showed that SDE didn't exhibit obvious cytotoxicity at the concentration of 3.125, 6.25 12.5 and 25 μg/ml. Subsequently, 25 μg/ml SDE was used for the further experiments. We found that IL-1β, IL-6 and NLRP3 mRNA levels in cells and protein levels in supernatant were all significantly increased in LPS combined with ATP-stimulated U937 cells compared to the control group. SDE could significantly downregulate the mRNA and protein levels of IL-1β, IL-6 and NLRP3 in SDE-treated groups compared to the LPS combined with ATP-stimulated group. Conclusion: These results showed that SDE could inhibit the production of IL-1β, IL-6 and NLRP3 in LPS combined with ATP-stimulated U937 cells.
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