大孔纳米纤维PLLA/PLCL支架的制备
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- 英文论文题名：The Fabrication of Macroporous and Nanofibrous PLLA/PLCL Scaffold
- 论文作者：王伟忠 ; 何创龙
- 英文论文作者：Weizhong Wang ; Chuanglong He ; College of Chemistry ; Chemical Engineering and Biotechnology ; Donghua University
- 年：2016
- 作者机构：东华大学化学化工与生物工程学院;
- 论文关键词：PLLA ; PLCL ; 热致相分离 ; 大孔结构 ; 纳米纤维
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十二分会： 多孔功能材料
- 语种：中文
- 分类号：TB383.1;R318.08
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十二分会 ; 多孔功能材料
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：245k
- 原文格式：D
- 会议级别：全国
摘要
适合细胞渗透生长的大孔结构是组织工程支架一个非常重要的因素。在之前的研究中~[1],我们采用热致相分离(TIPS)技术制备了聚左旋乳酸(PLLA)/聚左旋乳酸己内酯共聚物(PLCL)纳米纤维支架,但其小孔结构限制了细胞向支架内的渗透生长,因此一定程度上延缓了组织再生修复进程。本研究中,我们同样采用TIPS技术,研究了溶剂、粗化效应以及不同的溶剂去除方法(冷冻干燥和水置换)对PLLA/PLCL支架微观形貌的影响,并制备出了大孔纳米纤维PLLA/PLCL支架(Fig.1)。结果表明:相比冷冻干燥法,采用水置换法有利于纳米纤维结构的形成;溶剂中1,4-dioxane的加入可以导致大孔结构的形成,并且孔径随1,4-dioxane含量增加而增大,达到了20μm以上;粗化效应可以进一步促进纳米纤维结构的形成。这种大孔纳米纤维PLLA/PLCL支架有望应用于组织工程。
The macropores suitable for cell infiltration are critical to tissue engineering scaffold.In our previous study~([1]),poly(L-lactic acid)(PLLA)/poly(L-lactide-co-ε-caprolactone)(PLCL) nanofibrous scaffold was developed by using thermally induced phase separation(TIPS) technology.But its micropores limited the ingrowth of cells,and hindered the regeneration of new tissue.Herein,we studied the effect of solvent,coarsening process and different ways to remove solvent on the microstructure of PLLA/PLCL scaffold by using the same TIPS,and fabricated macroporous and nanofibrous PLLA/PLCL scaffold(Fig.1).The results indicated that solvent-exchanging by water can facilitate the formation of nanofibers in the scaffold compared to freeze-drying.The addition of 1,4-dioxane leaded to the formation of macropores,which size increased to more than 20 μm with the increase of 1,4-dioxane.In addition,coarsening effect promoted further the formation of nanofibers in the scaffold.The as-prepared macroporous and nanofibrous PLLA/PLCL scaffold has the potential for tissue engineering application.
The macropores suitable for cell infiltration are critical to tissue engineering scaffold.In our previous study~([1]),poly(L-lactic acid)(PLLA)/poly(L-lactide-co-ε-caprolactone)(PLCL) nanofibrous scaffold was developed by using thermally induced phase separation(TIPS) technology.But its micropores limited the ingrowth of cells,and hindered the regeneration of new tissue.Herein,we studied the effect of solvent,coarsening process and different ways to remove solvent on the microstructure of PLLA/PLCL scaffold by using the same TIPS,and fabricated macroporous and nanofibrous PLLA/PLCL scaffold(Fig.1).The results indicated that solvent-exchanging by water can facilitate the formation of nanofibers in the scaffold compared to freeze-drying.The addition of 1,4-dioxane leaded to the formation of macropores,which size increased to more than 20 μm with the increase of 1,4-dioxane.In addition,coarsening effect promoted further the formation of nanofibers in the scaffold.The as-prepared macroporous and nanofibrous PLLA/PLCL scaffold has the potential for tissue engineering application.
引文
[1]Wang,WZ.;Hu,JW.;He,CL.;Nie,W;et al.J.Biomed.Mater.Res.Part A.2015,103A:1784.