ICK5, a gene encoding a CDK inhibitor is involved in promotion of leaf senescence in Arabidopsis
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- 英文论文题名：ICK5, a gene encoding a CDK inhibitor is involved in promotion of leaf senescence in Arabidopsis
- 论文作者：Xiaoming Gao ; Yongfeng Guo ; Susheng Gan
- 英文论文作者：Xiaoming Gao ; Yongfeng Guo ; Susheng Gan ; Tobacco Research Institute ; Chinese Academy of Agricultural Sciences ; Department of Horticulture ; 119 Plant Science ; Cornell University
- 年：2016
- 作者机构：Tobacco Research Institute,Chinese Academy of Agricultural Sciences;Department of Horticulture,119 Plant Science,Cornell University;
- 英文论文关键词：AtICK5 ; leaf senescence ; AtNAP ; Arabidopsis
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：840k
- 原文格式：D
- 会议级别：全国
摘要
ICK5 is one of the ICK family genes encoding CDK(cyclin-dependent kinase) inhibitor,which are negative regulators of cell division. AtNAP is a NAC family transcription factor gene that plays a key role in leaf senescence,but its mechanisms are not well elucidated. Here we report that AtICK5 is a direct target gene of the AtNAP transcription factor,and expression of AtICK5 was induced by leaf senescence. Leaf senescence in one T-DNA insertion line of AtICK5 is significantly delayed. The TDNA knockout plants are otherwise normal. The mutant phenotype can be restored to wild-type by the intact AtICK5. Overexpression of AtICK5 resulted in dwarf plants. When AtNAP was chemically induced,AtICK5 was also induced,and the expression of AtICK5 was significantly reduced in atnap. These data suggest that the expression of AtICK5 is predominantly dependent on AtNAP. Yeast onehybrid experiments showed that AtNAP could physically bind to AtICK5 promoter in vitro. These data suggest that AtICK5 as a direct target gene of AtNAP plays an important role in leaf senescence in Arabidopsis.
ICK5 is one of the ICK family genes encoding CDK(cyclin-dependent kinase) inhibitor,which are negative regulators of cell division. AtNAP is a NAC family transcription factor gene that plays a key role in leaf senescence,but its mechanisms are not well elucidated. Here we report that AtICK5 is a direct target gene of the AtNAP transcription factor,and expression of AtICK5 was induced by leaf senescence. Leaf senescence in one T-DNA insertion line of AtICK5 is significantly delayed. The TDNA knockout plants are otherwise normal. The mutant phenotype can be restored to wild-type by the intact AtICK5. Overexpression of AtICK5 resulted in dwarf plants. When AtNAP was chemically induced,AtICK5 was also induced,and the expression of AtICK5 was significantly reduced in atnap. These data suggest that the expression of AtICK5 is predominantly dependent on AtNAP. Yeast onehybrid experiments showed that AtNAP could physically bind to AtICK5 promoter in vitro. These data suggest that AtICK5 as a direct target gene of AtNAP plays an important role in leaf senescence in Arabidopsis.
ICK5 is one of the ICK family genes encoding CDK(cyclin-dependent kinase) inhibitor,which are negative regulators of cell division. AtNAP is a NAC family transcription factor gene that plays a key role in leaf senescence,but its mechanisms are not well elucidated. Here we report that AtICK5 is a direct target gene of the AtNAP transcription factor,and expression of AtICK5 was induced by leaf senescence. Leaf senescence in one T-DNA insertion line of AtICK5 is significantly delayed. The TDNA knockout plants are otherwise normal. The mutant phenotype can be restored to wild-type by the intact AtICK5. Overexpression of AtICK5 resulted in dwarf plants. When AtNAP was chemically induced,AtICK5 was also induced,and the expression of AtICK5 was significantly reduced in atnap. These data suggest that the expression of AtICK5 is predominantly dependent on AtNAP. Yeast onehybrid experiments showed that AtNAP could physically bind to AtICK5 promoter in vitro. These data suggest that AtICK5 as a direct target gene of AtNAP plays an important role in leaf senescence in Arabidopsis.
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