Function analysis of interaction between host cell Vimentin and T. gondii ROP18
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- 英文论文题名：Function analysis of interaction between host cell Vimentin and T. gondii ROP18
- 论文作者：Cheng He ; Hongjuan Peng
- 英文论文作者：Cheng He ; Hongjuan Peng ; Department of Pathogen Biology ; Guangdong Provincial Key Laboratory of Tropical Disease Research ; School of Public Health and Tropical Medicine ; Southern Medical University
- 年：2016
- 作者机构：Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health and Tropical Medicine, Southern Medical University;
- 英文论文关键词：Toxoplasma gondii ; ROP18 ; Vimentin
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R382.5
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：338k
- 原文格式：D
- 会议级别：全国
摘要
Objectives: Vimentin, a class III intermediate filament protein, is involved in many cellular processes. Recent studies have revealed the involvement of vimentin in host cell defenses and it plays an important role in resistance to pathogen invasion and so on. But the role of vimentin in the process of T. gondii infection was unknown. Therefore we performed this study to explore the role of vimentin in the T. gondii infection process. Methods: We adopted FRET to identify the interaction between vimentin and ROP18. A significant rearrangement of host cell vimentin around the toxoplasma parasitophorous vacuoles occurs throughour the course of infected, which was revealed by immunofluorescence. To identify the role of vimentin further, RNA interference was adopted to knockdown the expression level. Additionally, we infected Cos-7 cells with T. gondii and the mutant with ROP18 knockout or transfected with ROP18 and the mutant, samples were then collected to perform vimentin solubility assays with western blot. Results: There was an interaction between vimentin and ROP18. A significant rearrangement and phosphorylation level change of vimentin was occurred when host cells were infected with T. gondii. The knockdown of vimentin didn't affect parasite replication. Conclusions: Interaction between vimentin and ROP18 was existed and there was a significant rearrangement as well as phosphorylation level change of host cell vimentin with T. gondii infection. But parasite replication wasn't affected when vimentin was knocked down.
Objectives: Vimentin, a class III intermediate filament protein, is involved in many cellular processes. Recent studies have revealed the involvement of vimentin in host cell defenses and it plays an important role in resistance to pathogen invasion and so on. But the role of vimentin in the process of T. gondii infection was unknown. Therefore we performed this study to explore the role of vimentin in the T. gondii infection process. Methods: We adopted FRET to identify the interaction between vimentin and ROP18. A significant rearrangement of host cell vimentin around the toxoplasma parasitophorous vacuoles occurs throughour the course of infected, which was revealed by immunofluorescence. To identify the role of vimentin further, RNA interference was adopted to knockdown the expression level. Additionally, we infected Cos-7 cells with T. gondii and the mutant with ROP18 knockout or transfected with ROP18 and the mutant, samples were then collected to perform vimentin solubility assays with western blot. Results: There was an interaction between vimentin and ROP18. A significant rearrangement and phosphorylation level change of vimentin was occurred when host cells were infected with T. gondii. The knockdown of vimentin didn't affect parasite replication. Conclusions: Interaction between vimentin and ROP18 was existed and there was a significant rearrangement as well as phosphorylation level change of host cell vimentin with T. gondii infection. But parasite replication wasn't affected when vimentin was knocked down.
Objectives: Vimentin, a class III intermediate filament protein, is involved in many cellular processes. Recent studies have revealed the involvement of vimentin in host cell defenses and it plays an important role in resistance to pathogen invasion and so on. But the role of vimentin in the process of T. gondii infection was unknown. Therefore we performed this study to explore the role of vimentin in the T. gondii infection process. Methods: We adopted FRET to identify the interaction between vimentin and ROP18. A significant rearrangement of host cell vimentin around the toxoplasma parasitophorous vacuoles occurs throughour the course of infected, which was revealed by immunofluorescence. To identify the role of vimentin further, RNA interference was adopted to knockdown the expression level. Additionally, we infected Cos-7 cells with T. gondii and the mutant with ROP18 knockout or transfected with ROP18 and the mutant, samples were then collected to perform vimentin solubility assays with western blot. Results: There was an interaction between vimentin and ROP18. A significant rearrangement and phosphorylation level change of vimentin was occurred when host cells were infected with T. gondii. The knockdown of vimentin didn't affect parasite replication. Conclusions: Interaction between vimentin and ROP18 was existed and there was a significant rearrangement as well as phosphorylation level change of host cell vimentin with T. gondii infection. But parasite replication wasn't affected when vimentin was knocked down.
引文