广州木棉种质资源遗传多样性SSR分析
详细信息 查看官网全文
- 英文论文题名：Genetic Diversity Analysis on Bombax ceiba in Guangzhou Based on SSR Markers
- 论文作者：王伟 ; 倪建中 ; 贺漫媚 ; 张继方 ; 代色平
- 英文论文作者：WANG Wei ; NI Jian-zhong ; HE Man-mei ; ZHANG Ji-fang ; DAI Se-ping ; Academy of Forestry and Gardening of Guangzhou/ Municipal Key Laboratory of Landscape Architecture
- 年：2016
- 作者机构：广州市林业和园林科学研究院/广州市景观建筑重点实验室;
- 论文关键词：木棉 ; 种质资源 ; 遗传多样性 ; SSR
- 英文论文关键词：Bombax ceiba ; Germplasm resources ; Genetic diversity ; SSR
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S685.99
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：7
- 文件大小：569k
- 原文格式：O
- 会议级别：全国
摘要
本研究利用SSR标记对100个广州木棉单株进行木棉遗传多样性分析,目的是研究广州地区木棉在遗传多样性水平上的差异和分化情况。结果表明,从99对木棉SSR标记种筛选出11对具有多态性的引物,共检测出37个多态性位点,每对引物的等位变异为2~6个,平均为3.23个;木棉群体的Ne变化范围为1.0~3.1591,平均值为1.8954,居群间的Shannon信息指数(Ⅰ)为0.6868,Nei's基因多样性指数(He)为0.4175。根据UPGMA分析,基于遗传距离0.416处将100份材料分为5大类群,结果表明,广州木棉亲缘关系与采集地和表型无显著相关性。
To reveal difference and characteristics of Bombax ceiba in Guangzhou on the level of genetic diversity.SSR molecular markers technique were used to study genetic diversity of 100 single individual plants.The result showed that 37 alleles were detected using 11 polymorphic SSR primers among the 100 accessions.2 to 6 alleles were indentified among different loci and the average number of alleles per marker across genotypes was 3.23.Total Nei' s genetic diversity index varied greatly from 1.0to 3.1591,with an average of 1.8954.The Shannon' s information(I) and the average index of Nei' s genetic diversity(He)were 0.6868 and 0.4175 respectively.The 100 single individual plants were divided into 5 groups at the threshold of 0.416 based on genetic distance,showed that the genetic distance was no significantly difference with the geographic latitude and phenotype of Bombax ceiba.
To reveal difference and characteristics of Bombax ceiba in Guangzhou on the level of genetic diversity.SSR molecular markers technique were used to study genetic diversity of 100 single individual plants.The result showed that 37 alleles were detected using 11 polymorphic SSR primers among the 100 accessions.2 to 6 alleles were indentified among different loci and the average number of alleles per marker across genotypes was 3.23.Total Nei' s genetic diversity index varied greatly from 1.0to 3.1591,with an average of 1.8954.The Shannon' s information(I) and the average index of Nei' s genetic diversity(He)were 0.6868 and 0.4175 respectively.The 100 single individual plants were divided into 5 groups at the threshold of 0.416 based on genetic distance,showed that the genetic distance was no significantly difference with the geographic latitude and phenotype of Bombax ceiba.
引文
1.Akfirat F S,Uncuoglu A A.Genetic diversity of winter wheat(Triticum aestivum L.)revealed by SSR markers[J].Biochem Genet,2013,51(3-4):223-229.
2.Cockerham C C,Weir B S.Estimation of gene-flow from Fstatistics[J].Evolution,1993,47:855-863.
3.Powell W,Morgante M,Andre C,et al.The comparison of RFLP,RAPD,AFLP and SSR(microsatellite)markers for germplasm analysis[J].Mol Breeding,1996,2(3):225-238.
4.Thenmozhi P,Rajasekaran C.Genetic diversity and relationship among 40 rice accessions from northeastern zone of tamil nadu using morphological and SSR markers[J].Res J Biotechnol,2013,8(9):32-41.
5.董研,张军,任亚超,等.中国新疆苹果天然群体遗传多样性SSR分析[J].植物资源学报,2013,14(5):771-777.
6.赖勇,王鹏喜,范贵强,等.大麦SSR标记遗传多样性及其与农艺性状关联分析[J].中国农业科学,2013,46(2):233-242.
7.李鸿雁,李志勇,师文贵,等.3种生态型野生扁蓿豆种质资源ISSR与SSR遗传多样性分析[J].草叶学报,2012,21(5):107-113.
8.彭莉霞,朱报著,杨会肖,等.广东省木棉种质资源ISSR遗传多样性分析[J].广东林业科技,2015,31(6):23-28.
9.汪书丽.云南干热河谷地区木棉群居的遗传多样性研究[J].西双版纳:中国科学院,2007.
10.张自强,于肖夏,鞠天华,等.36个马铃薯品种的SSR分析[J].华北农学报,2012,27(1):93-97.
2.Cockerham C C,Weir B S.Estimation of gene-flow from Fstatistics[J].Evolution,1993,47:855-863.
3.Powell W,Morgante M,Andre C,et al.The comparison of RFLP,RAPD,AFLP and SSR(microsatellite)markers for germplasm analysis[J].Mol Breeding,1996,2(3):225-238.
4.Thenmozhi P,Rajasekaran C.Genetic diversity and relationship among 40 rice accessions from northeastern zone of tamil nadu using morphological and SSR markers[J].Res J Biotechnol,2013,8(9):32-41.
5.董研,张军,任亚超,等.中国新疆苹果天然群体遗传多样性SSR分析[J].植物资源学报,2013,14(5):771-777.
6.赖勇,王鹏喜,范贵强,等.大麦SSR标记遗传多样性及其与农艺性状关联分析[J].中国农业科学,2013,46(2):233-242.
7.李鸿雁,李志勇,师文贵,等.3种生态型野生扁蓿豆种质资源ISSR与SSR遗传多样性分析[J].草叶学报,2012,21(5):107-113.
8.彭莉霞,朱报著,杨会肖,等.广东省木棉种质资源ISSR遗传多样性分析[J].广东林业科技,2015,31(6):23-28.
9.汪书丽.云南干热河谷地区木棉群居的遗传多样性研究[J].西双版纳:中国科学院,2007.
10.张自强,于肖夏,鞠天华,等.36个马铃薯品种的SSR分析[J].华北农学报,2012,27(1):93-97.