Genetic polymorphism among Arabidopsis thaliana mutants induced by carbon ion beam irradiations
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- 英文论文题名：Genetic polymorphism among Arabidopsis thaliana mutants induced by carbon ion beam irradiations
- 论文作者：Hui Feng ; Wenjian Li ; Yan Du ; Shanwei Luo ; Lixia Yu ; Yuze Chen ; Jinhu Mu ; Libin Zhou
- 英文论文作者：Hui Feng ; Wenjian Li ; Yan Du ; Shanwei Luo ; Lixia Yu ; Yuze Chen ; Jinhu Mu ; Libin Zhou ; Department of Biophysics ; Institute of Modern Physics ; Chinese Academy of Sciences
- 年：2016
- 作者机构：Department of Biophysics,Institute of Modern Physics,Chinese Academy of Sciences;
- 英文论文关键词：carbon ion beams ; Arabidopsis thaliana ; Inter-simple sequence repeat(ISSR) ; random amplified polymorphic DNA(RAPD) ; genetic polymorphism
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：852k
- 原文格式：D
- 会议级别：全国
摘要
Arabidopsis thaliana is a member of crucifer family and has become an important model plant in plant sciences,which is widely used in genetics and molecular biology. As a novel mutagen,heavy ion beams play an important role in mutation breeding. In the present study,in order to explore the effects of heavy ion beam irradiations on Arabidopsis thaliana,the wild-type(ecotype Columbia,Col) and A. thaliana M3 plants induced by carbon ion beam irradiations(with the energy of 43.3 Me V/u and linear energy transfer(LET) of 50 ke V/μm) were used for analyzing genetic polymorphisms by inter-simple sequence repeat(ISSR) markers and random amplified polymorphic DNA(RAPD) markers. Compared with wild-type,either ISSR or RAPD patterns,the major forms of genetic polymorphism variations of the M3 plants were appearance of new bands and disappearance of normal bands. The total polymorphism rates were 17.06%(ISSR) and 21.24%(RAPD) among M3 plants,and the genetic similarity coefficient between the M3 plants and wild-type distributed from 0.65 to 0.96(ISSR) and 0.74 to 0.91(RAPD). Dendrograms constructed by UPGMA method divided the genotypes into two clusters by ISSR and RAPD techniques,respectively. In addition,the connection between genetic polymorphism and phenotypic changes of carbon-ion beam induced A. thaliana mutants in laboratory conditions were investigated. In summary,the present results showed that carbon ion beam irradiations are efficient mutagens on account of its ability to induce heritable genetic polymorphism which lead to diversified mutations,and the results could provide theoretical foundations for irradiations mutation breeding to improve better genotypes and produce better varieties with higher yield or other characters.
Arabidopsis thaliana is a member of crucifer family and has become an important model plant in plant sciences,which is widely used in genetics and molecular biology. As a novel mutagen,heavy ion beams play an important role in mutation breeding. In the present study,in order to explore the effects of heavy ion beam irradiations on Arabidopsis thaliana,the wild-type(ecotype Columbia,Col) and A. thaliana M3 plants induced by carbon ion beam irradiations(with the energy of 43.3 Me V/u and linear energy transfer(LET) of 50 ke V/μm) were used for analyzing genetic polymorphisms by inter-simple sequence repeat(ISSR) markers and random amplified polymorphic DNA(RAPD) markers. Compared with wild-type,either ISSR or RAPD patterns,the major forms of genetic polymorphism variations of the M3 plants were appearance of new bands and disappearance of normal bands. The total polymorphism rates were 17.06%(ISSR) and 21.24%(RAPD) among M3 plants,and the genetic similarity coefficient between the M3 plants and wild-type distributed from 0.65 to 0.96(ISSR) and 0.74 to 0.91(RAPD). Dendrograms constructed by UPGMA method divided the genotypes into two clusters by ISSR and RAPD techniques,respectively. In addition,the connection between genetic polymorphism and phenotypic changes of carbon-ion beam induced A. thaliana mutants in laboratory conditions were investigated. In summary,the present results showed that carbon ion beam irradiations are efficient mutagens on account of its ability to induce heritable genetic polymorphism which lead to diversified mutations,and the results could provide theoretical foundations for irradiations mutation breeding to improve better genotypes and produce better varieties with higher yield or other characters.
Arabidopsis thaliana is a member of crucifer family and has become an important model plant in plant sciences,which is widely used in genetics and molecular biology. As a novel mutagen,heavy ion beams play an important role in mutation breeding. In the present study,in order to explore the effects of heavy ion beam irradiations on Arabidopsis thaliana,the wild-type(ecotype Columbia,Col) and A. thaliana M3 plants induced by carbon ion beam irradiations(with the energy of 43.3 Me V/u and linear energy transfer(LET) of 50 ke V/μm) were used for analyzing genetic polymorphisms by inter-simple sequence repeat(ISSR) markers and random amplified polymorphic DNA(RAPD) markers. Compared with wild-type,either ISSR or RAPD patterns,the major forms of genetic polymorphism variations of the M3 plants were appearance of new bands and disappearance of normal bands. The total polymorphism rates were 17.06%(ISSR) and 21.24%(RAPD) among M3 plants,and the genetic similarity coefficient between the M3 plants and wild-type distributed from 0.65 to 0.96(ISSR) and 0.74 to 0.91(RAPD). Dendrograms constructed by UPGMA method divided the genotypes into two clusters by ISSR and RAPD techniques,respectively. In addition,the connection between genetic polymorphism and phenotypic changes of carbon-ion beam induced A. thaliana mutants in laboratory conditions were investigated. In summary,the present results showed that carbon ion beam irradiations are efficient mutagens on account of its ability to induce heritable genetic polymorphism which lead to diversified mutations,and the results could provide theoretical foundations for irradiations mutation breeding to improve better genotypes and produce better varieties with higher yield or other characters.
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