陆地棉野生种与栽培种间纤维发育差异的蛋白质组学研究（英文）
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- 英文论文题名：Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L.
- 论文作者：覃元 ; 魏恒玲 ; 孙会茹 ; 郝蓬勃 ; 王寒涛 ; 宿俊吉 ; 喻树迅
- 英文论文作者：Qin Yuan ; Wei Hengling ; Sun Huiru ; Hao Pengbo ; Wang Hantao ; Su Junji ; Yu Shuxun ; State Key Laboratory of Cotton Biology/Institute of Cotton Research ; Chinese Academy of Agricultural Sciences
- 年：2017
- 作者机构：中国农业科学院棉花研究所/棉花生物学国家重点实验室;
- 英文论文关键词：Gossypium hirsutum ; fiber development ; domestication ; iTRAQ ; phenylpropanoid ; zeatin
- 会议召开时间：2017-08-07
- 会议录名称：中国农学会棉花分会2017年年会暨第九次会员代表大会论文汇编
- 语种：英文
- 分类号：S562
- 学会代码：MHXB
- 会议名称：中国农学会棉花分会2017年年会暨第九次会员代表大会
- 会议地点：中国河南郑州
- 主办单位：中国农学会棉花分会
- 学会名称：《棉花学报》编辑部
- 页数：1
- 文件大小：47k
- 原文格式：O
- 会议级别：全国
摘要
Upland cotton(Gossypium hirsutum L.) is one of the world's most important fiber crops,accounting for more than 90%of all cotton production.While their wild progenitors have relatively short and coarse,often tan-colored fibers,modern cotton cultivars possess longer,finer,stronger,and whiter fiber.In this study,the wild and cultivated cottons(YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis(DPA),20 DPA and mature stages at the morphological level.In order to explore the effects of domestication,reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality,an iTRAQ-facilitated proteomic method was performed on developing fibers.There were 6990 proteins identified,among them 336 were defined as differentially expressed proteins(DEPs) between fibers of wild versus domesticated cotton.The down-or up-regulated proteins in wild cotton were involved in phenylpropanoid biosynthesis,zeatin biosynthesis,fatty acid elongation and other processes.Association analysis between transcriptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 DPA.Differences in proteomics have been verified at the mRNA level by qPCR and have been validated at the physiological and biochemical levels by POD(peroxidase) activity assays and ZA(zeatin) content estimates.This work corroborates the major pathways involved in cotton fiber development and demonstrates that POD activity and zeatin content have a great potential related to fiber elongation and thickening.
Upland cotton(Gossypium hirsutum L.) is one of the world's most important fiber crops,accounting for more than 90%of all cotton production.While their wild progenitors have relatively short and coarse,often tan-colored fibers,modern cotton cultivars possess longer,finer,stronger,and whiter fiber.In this study,the wild and cultivated cottons(YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis(DPA),20 DPA and mature stages at the morphological level.In order to explore the effects of domestication,reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality,an iTRAQ-facilitated proteomic method was performed on developing fibers.There were 6990 proteins identified,among them 336 were defined as differentially expressed proteins(DEPs) between fibers of wild versus domesticated cotton.The down-or up-regulated proteins in wild cotton were involved in phenylpropanoid biosynthesis,zeatin biosynthesis,fatty acid elongation and other processes.Association analysis between transcriptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 DPA.Differences in proteomics have been verified at the mRNA level by qPCR and have been validated at the physiological and biochemical levels by POD(peroxidase) activity assays and ZA(zeatin) content estimates.This work corroborates the major pathways involved in cotton fiber development and demonstrates that POD activity and zeatin content have a great potential related to fiber elongation and thickening.
Upland cotton(Gossypium hirsutum L.) is one of the world's most important fiber crops,accounting for more than 90%of all cotton production.While their wild progenitors have relatively short and coarse,often tan-colored fibers,modern cotton cultivars possess longer,finer,stronger,and whiter fiber.In this study,the wild and cultivated cottons(YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis(DPA),20 DPA and mature stages at the morphological level.In order to explore the effects of domestication,reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality,an iTRAQ-facilitated proteomic method was performed on developing fibers.There were 6990 proteins identified,among them 336 were defined as differentially expressed proteins(DEPs) between fibers of wild versus domesticated cotton.The down-or up-regulated proteins in wild cotton were involved in phenylpropanoid biosynthesis,zeatin biosynthesis,fatty acid elongation and other processes.Association analysis between transcriptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 DPA.Differences in proteomics have been verified at the mRNA level by qPCR and have been validated at the physiological and biochemical levels by POD(peroxidase) activity assays and ZA(zeatin) content estimates.This work corroborates the major pathways involved in cotton fiber development and demonstrates that POD activity and zeatin content have a great potential related to fiber elongation and thickening.
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