Gene expression profiling of CD8~+ T cells induced by ovarian cancer cells suggests a possible mechanism for CD8~+ Treg cell production
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- 英文论文题名：Gene expression profiling of CD8~+ T cells induced by ovarian cancer cells suggests a possible mechanism for CD8~+ Treg cell production
- 论文作者：Wu Meng ; Chen Xian ; Lou Jianfang ; Huang Lei ; Sun Ruihong ; Huang Peijun ; Wang Fang
- 英文论文作者：Wu Meng ; Chen Xian ; Lou Jianfang ; Huang Lei ; Sun Ruihong ; Huang Peijun ; Wang Fang ; the First Affiliated Hospital of Nanjing Medical University
- 年：2016
- 作者机构：the First Affiliated Hospital of Nanjing Medical University;
- 英文论文关键词：CD8~+ T cell ; microarray ; ovarian cancer ; regulatory T cell
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R737.31
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：271k
- 原文格式：D
- 会议级别：全国
摘要
Objectives:The focus of this study was to investigate a possible mechanism of CD8~+ regulatory T cell(Treg) production in ovarian cancer(OC) microenvironment.Methods:Agilent microarray was used to detect changes in gene expression between CD8~+ T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line.RT-q PCR was performed to determine expression of glycolysis gene expression in CD8~+ T cells from transwell culturing system and OC patients.We also detected the protein level of glycolysis related genes using western blot.Results:Comparing gene expression profiles revealed significant differences in the expression levels of 1,420 genes,of which 246 were upregulated and 1,174 were downregulated.Gene ontology and Kyoto encyclopedia of genes and genomes analysis indicated that biological processes altered in CD8~+ Treg are particularly associated with energy metabolism.CD8~+ Treg cells induced by co-culture with SKOV3 had decreased glycolysis gene expression compared with CD8~+T cells cultured alone.Glycolysis gene expression was also decreased in the CD8~+ T cells of ovarian cancer patients.Conclusions:These findings provide a comprehensive bioinformatics analysis of DEGs in CD8~+ T cells cultured with and without the SKOV3,and suggest that metabolic processes may be a possible mechanism for CD8~+ Treg induction.
Objectives:The focus of this study was to investigate a possible mechanism of CD8~+ regulatory T cell(Treg) production in ovarian cancer(OC) microenvironment.Methods:Agilent microarray was used to detect changes in gene expression between CD8~+ T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line.RT-q PCR was performed to determine expression of glycolysis gene expression in CD8~+ T cells from transwell culturing system and OC patients.We also detected the protein level of glycolysis related genes using western blot.Results:Comparing gene expression profiles revealed significant differences in the expression levels of 1,420 genes,of which 246 were upregulated and 1,174 were downregulated.Gene ontology and Kyoto encyclopedia of genes and genomes analysis indicated that biological processes altered in CD8~+ Treg are particularly associated with energy metabolism.CD8~+ Treg cells induced by co-culture with SKOV3 had decreased glycolysis gene expression compared with CD8~+T cells cultured alone.Glycolysis gene expression was also decreased in the CD8~+ T cells of ovarian cancer patients.Conclusions:These findings provide a comprehensive bioinformatics analysis of DEGs in CD8~+ T cells cultured with and without the SKOV3,and suggest that metabolic processes may be a possible mechanism for CD8~+ Treg induction.
Objectives:The focus of this study was to investigate a possible mechanism of CD8~+ regulatory T cell(Treg) production in ovarian cancer(OC) microenvironment.Methods:Agilent microarray was used to detect changes in gene expression between CD8~+ T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line.RT-q PCR was performed to determine expression of glycolysis gene expression in CD8~+ T cells from transwell culturing system and OC patients.We also detected the protein level of glycolysis related genes using western blot.Results:Comparing gene expression profiles revealed significant differences in the expression levels of 1,420 genes,of which 246 were upregulated and 1,174 were downregulated.Gene ontology and Kyoto encyclopedia of genes and genomes analysis indicated that biological processes altered in CD8~+ Treg are particularly associated with energy metabolism.CD8~+ Treg cells induced by co-culture with SKOV3 had decreased glycolysis gene expression compared with CD8~+T cells cultured alone.Glycolysis gene expression was also decreased in the CD8~+ T cells of ovarian cancer patients.Conclusions:These findings provide a comprehensive bioinformatics analysis of DEGs in CD8~+ T cells cultured with and without the SKOV3,and suggest that metabolic processes may be a possible mechanism for CD8~+ Treg induction.
引文