Changes in regulatory T cells in patients with ovarian cancer undergoing surgery:preliminary results
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- 英文论文题名：Changes in regulatory T cells in patients with ovarian cancer undergoing surgery:preliminary results
- 论文作者：Wu Meng ; Chen Xian ; Lou Jianfang ; Huang Lei ; Sun Ruihong ; Huang Peijun ; Wang Fang
- 英文论文作者：Wu Meng ; Chen Xian ; Lou Jianfang ; Huang Lei ; Sun Ruihong ; Huang Peijun ; Wang Fang ; the First Affiliated Hospital of Nanjing Medical University
- 年：2016
- 作者机构：the First Affiliated Hospital of Nanjing Medical University;
- 英文论文关键词：ovarian cancer ; regulatory T cell ; surgery
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R737.31
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1335k
- 原文格式：D
- 会议级别：全国
摘要
Objective:Regulatory T cells(Treg) suppress immune responses in patients with cancer.Surgery is the most effective therapeutic strategy for ovarian cancer(OC).However,the interplay between the Treg population and surgical resection remains unclear.The aim of this study is to measure the alterations of the Treg population in the peripheral blood of OC patients undergoing surgery.Methods:61 patients with OC who received no prior treatment were enrolled in the study.We investigated CD4~+CD25~+,CD4~+CD25~+Foxp3~+,CD8~+CD28,and CD8~+Foxp3~+ Treg cells in patients with OC and their postoperative changes using flow cytometry.Proliferation assays was performed to explore the function of T cells in OC patients,benign ovarian tumors(BOT) patients and healthy controls(HC).Results:Treg percentages were significantly higher in patients with OC than those in BOT and healthy controls.Higherpercentages of Tregs were found in patients with stage III/IV than stage I/II OC.Proliferation assays proved that the T cells isolated from OC patients had the functional characteristics of Treg cells.Treg percentages were significantly decreased postoperatively.The postoperative Treg percentages in patients with stage I/II OC were not significantly different from those in BOT patients,while postoperative Treg percentages in patients with stage III/IV OC remained higher than those in BOT patients.Treg percentages were markedly lower on postoperative day(POD) 3 than preoperatively.They increased slightly after 7 days,but remained lower than preoperative levels.Treg percentages then continued to decline from POD7 to POD30.Conclusion:The study confirmed an elevated level of CD4~+CD25~+,CD4~+CD25~+Foxp3~+,CD8~+CD28~+,and CD8~+Foxp3~+ Treg cells in patients with OC,even in the early stages of the disease.Surgery is able to partially revert the immunosuppressive state of patients with cancer because diminishment of the Treg level was found in patients with OC after surgery.Our research suggests that precise evaluation of the Treg
Objective:Regulatory T cells(Treg) suppress immune responses in patients with cancer.Surgery is the most effective therapeutic strategy for ovarian cancer(OC).However,the interplay between the Treg population and surgical resection remains unclear.The aim of this study is to measure the alterations of the Treg population in the peripheral blood of OC patients undergoing surgery.Methods:61 patients with OC who received no prior treatment were enrolled in the study.We investigated CD4~+CD25~+,CD4~+CD25~+Foxp3~+,CD8~+CD28,and CD8~+Foxp3~+ Treg cells in patients with OC and their postoperative changes using flow cytometry.Proliferation assays was performed to explore the function of T cells in OC patients,benign ovarian tumors(BOT) patients and healthy controls(HC).Results:Treg percentages were significantly higher in patients with OC than those in BOT and healthy controls.Higherpercentages of Tregs were found in patients with stage III/IV than stage I/II OC.Proliferation assays proved that the T cells isolated from OC patients had the functional characteristics of Treg cells.Treg percentages were significantly decreased postoperatively.The postoperative Treg percentages in patients with stage I/II OC were not significantly different from those in BOT patients,while postoperative Treg percentages in patients with stage III/IV OC remained higher than those in BOT patients.Treg percentages were markedly lower on postoperative day(POD) 3 than preoperatively.They increased slightly after 7 days,but remained lower than preoperative levels.Treg percentages then continued to decline from POD7 to POD30.Conclusion:The study confirmed an elevated level of CD4~+CD25~+,CD4~+CD25~+Foxp3~+,CD8~+CD28~+,and CD8~+Foxp3~+ Treg cells in patients with OC,even in the early stages of the disease.Surgery is able to partially revert the immunosuppressive state of patients with cancer because diminishment of the Treg level was found in patients with OC after surgery.Our research suggests that precise evaluation of the Treg
Objective:Regulatory T cells(Treg) suppress immune responses in patients with cancer.Surgery is the most effective therapeutic strategy for ovarian cancer(OC).However,the interplay between the Treg population and surgical resection remains unclear.The aim of this study is to measure the alterations of the Treg population in the peripheral blood of OC patients undergoing surgery.Methods:61 patients with OC who received no prior treatment were enrolled in the study.We investigated CD4~+CD25~+,CD4~+CD25~+Foxp3~+,CD8~+CD28,and CD8~+Foxp3~+ Treg cells in patients with OC and their postoperative changes using flow cytometry.Proliferation assays was performed to explore the function of T cells in OC patients,benign ovarian tumors(BOT) patients and healthy controls(HC).Results:Treg percentages were significantly higher in patients with OC than those in BOT and healthy controls.Higherpercentages of Tregs were found in patients with stage III/IV than stage I/II OC.Proliferation assays proved that the T cells isolated from OC patients had the functional characteristics of Treg cells.Treg percentages were significantly decreased postoperatively.The postoperative Treg percentages in patients with stage I/II OC were not significantly different from those in BOT patients,while postoperative Treg percentages in patients with stage III/IV OC remained higher than those in BOT patients.Treg percentages were markedly lower on postoperative day(POD) 3 than preoperatively.They increased slightly after 7 days,but remained lower than preoperative levels.Treg percentages then continued to decline from POD7 to POD30.Conclusion:The study confirmed an elevated level of CD4~+CD25~+,CD4~+CD25~+Foxp3~+,CD8~+CD28~+,and CD8~+Foxp3~+ Treg cells in patients with OC,even in the early stages of the disease.Surgery is able to partially revert the immunosuppressive state of patients with cancer because diminishment of the Treg level was found in patients with OC after surgery.Our research suggests that precise evaluation of the Treg
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