Identification and molecular survey of Borrelia burgdorferi in sika deer from Jilin Province in China
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- 英文论文题名：Identification and molecular survey of Borrelia burgdorferi in sika deer from Jilin Province in China
- 论文作者：Bintao Zhai ; Qingli Niu ; Jifei Yang ; Zhijie Liu ; Hong Yin ; Qiaoying Zeng
- 英文论文作者：Bintao Zhai ; Qingli Niu ; Jifei Yang ; Zhijie Liu ; Hong Yin ; Qiaoying Zeng ; The College of Veterinary Medicine ; Gansu Agricultural University ; State Key Laboratory of Veterinary Etiological Biology ; Key Laboratory of Veterinary Parasitology of Gansu Province ; Lanzhou Veterinary Research Institute ; Chinese Academy of Agricultural Sciences
- 年：2016
- 作者机构：The College of Veterinary Medicine, Gansu Agricultural University;State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;
- 英文论文关键词：Borrelia burgdorferi ; sika deer ; identification ; survey
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：S858.93
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：339k
- 原文格式：D
- 会议级别：全国
摘要
Background: Lyme disease is caused by Borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of Borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. The prevalence of Borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the Borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of Borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for Borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with Borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of Borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.
Background: Lyme disease is caused by Borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of Borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. The prevalence of Borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the Borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of Borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for Borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with Borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of Borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.
Background: Lyme disease is caused by Borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of Borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. The prevalence of Borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the Borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of Borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for Borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with Borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of Borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.
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